DE3750774D1 - Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. - Google Patents

Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.

Info

Publication number
DE3750774D1
DE3750774D1 DE3750774T DE3750774T DE3750774D1 DE 3750774 D1 DE3750774 D1 DE 3750774D1 DE 3750774 T DE3750774 T DE 3750774T DE 3750774 T DE3750774 T DE 3750774T DE 3750774 D1 DE3750774 D1 DE 3750774D1
Authority
DE
Germany
Prior art keywords
biological sample
nucleic acids
estimating
solution
chaotropic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3750774T
Other languages
English (en)
Other versions
DE3750774T2 (de
Inventor
David Gillespie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HAHNEMANN, University of
Hahnemann University
Original Assignee
HAHNEMANN, University of
Hahnemann University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HAHNEMANN, University of, Hahnemann University filed Critical HAHNEMANN, University of
Publication of DE3750774D1 publication Critical patent/DE3750774D1/de
Application granted granted Critical
Publication of DE3750774T2 publication Critical patent/DE3750774T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
DE3750774T 1986-05-02 1987-05-04 Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. Expired - Fee Related DE3750774T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85900386A 1986-05-02 1986-05-02
PCT/US1987/001023 WO1987006621A1 (en) 1986-05-02 1987-05-04 Chaotropic method for evaluating nucleic acids in a biological sample

Publications (2)

Publication Number Publication Date
DE3750774D1 true DE3750774D1 (de) 1995-01-05
DE3750774T2 DE3750774T2 (de) 1995-04-27

Family

ID=25329732

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3750774T Expired - Fee Related DE3750774T2 (de) 1986-05-02 1987-05-04 Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.

Country Status (7)

Country Link
EP (1) EP0305399B1 (de)
JP (1) JP2552691B2 (de)
AT (1) ATE114334T1 (de)
AU (1) AU613870B2 (de)
CA (1) CA1301606C (de)
DE (1) DE3750774T2 (de)
WO (1) WO1987006621A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008038A1 (en) * 1987-04-15 1988-10-20 Yissum Research Development Company Of The Hebrew Method for detecting the expression of bioregulatory genes in microcultures of eukaryotic cells and kit therefor
WO1990010717A1 (en) * 1989-03-10 1990-09-20 Gene-Trak Systems Preventing interference with affinity capture schemes
AU5401290A (en) * 1989-04-05 1990-11-05 Gene-Trak Systems Hybridization promotion reagents
US5589335A (en) * 1989-04-05 1996-12-31 Amoco Corporation Hybridization promotion reagents
AU6075490A (en) * 1989-07-11 1991-02-06 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5506350A (en) * 1990-09-20 1996-04-09 Bittner; Michael L. Production of chromosome region specific DNA sequences and transamination
US5459253A (en) * 1991-07-19 1995-10-17 Pharmacia P-L Biochemicals Inc. M-RNA purification
EP0554437B1 (de) * 1991-07-31 2000-04-05 VYSIS, Inc. Nukleinsäure sonden welche bei nachweis von shigellabakterien verwendbar sind
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
US6001559A (en) * 1992-07-13 1999-12-14 The Pillsbury Company Method for detecting Listeria monocytogenes via esculin hydrolysis and nucleic acid hybridization
WO1995002068A1 (fr) * 1993-07-09 1995-01-19 Wakunaga Seiyaku Kabushiki Kaisha Methode de discrimination des acides nucleiques et necessaire d'essai a cette fin
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
AT1082U2 (de) * 1996-07-09 1996-10-25 Labordiagnostika Ges Mbh Verfahren zur stabilisierung von nukleinsäuren vor deren isolierung aus blutproben
AU4599797A (en) * 1996-09-24 1998-04-17 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization specificity
EP0952228A3 (de) * 1996-09-24 1999-12-15 Rapigene, Inc. Zusammensetzungen und Verfahren zur Verbesserung der Hybridisierungsspezifität
US6361940B1 (en) 1996-09-24 2002-03-26 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization and priming specificity
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6210881B1 (en) 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
US20030096232A1 (en) 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
DE19956820A1 (de) 1999-11-25 2001-05-31 Roche Diagnostics Gmbh Spezies-spezifischer Nachweis von Nukleinsäuren mittels eines Analyseelementes
US6503716B1 (en) 2000-11-28 2003-01-07 Pe Corporation (Ny) Compositions and methods for extracting a nucleic acid
US8148171B2 (en) 2001-10-09 2012-04-03 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
AU2002359522A1 (en) 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
DE10261529A1 (de) * 2002-12-23 2004-07-08 Indivumed Gmbh Verfahren zur Erstellung einer Sammlung von biologischem Probenmaterial und Probensammlung
JP4831725B2 (ja) * 2004-02-13 2011-12-07 栄研化学株式会社 簡易的核酸抽出法
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
WO2010134245A1 (ja) * 2009-05-20 2010-11-25 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
CN105008534A (zh) * 2012-09-19 2015-10-28 贝克曼考尔特公司 在核酸的提取中使用二价离子、蛋白酶、清洁剂和低pH

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
US4427580A (en) * 1982-09-01 1984-01-24 Cornell Research Foundation, Inc. Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts

Also Published As

Publication number Publication date
AU7432987A (en) 1987-11-24
JPH01502317A (ja) 1989-08-17
CA1301606C (en) 1992-05-26
DE3750774T2 (de) 1995-04-27
ATE114334T1 (de) 1994-12-15
EP0305399B1 (de) 1994-11-23
EP0305399A4 (de) 1989-12-28
EP0305399A1 (de) 1989-03-08
WO1987006621A1 (en) 1987-11-05
JP2552691B2 (ja) 1996-11-13
AU613870B2 (en) 1991-08-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee