ATE114334T1 - Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. - Google Patents
Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.Info
- Publication number
- ATE114334T1 ATE114334T1 AT87903558T AT87903558T ATE114334T1 AT E114334 T1 ATE114334 T1 AT E114334T1 AT 87903558 T AT87903558 T AT 87903558T AT 87903558 T AT87903558 T AT 87903558T AT E114334 T1 ATE114334 T1 AT E114334T1
- Authority
- AT
- Austria
- Prior art keywords
- biological sample
- chaotropical
- estimation
- procedure
- solution
- Prior art date
Links
- 239000012472 biological sample Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 1
- 150000007513 acids Chemical class 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85900386A | 1986-05-02 | 1986-05-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE114334T1 true ATE114334T1 (de) | 1994-12-15 |
Family
ID=25329732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT87903558T ATE114334T1 (de) | 1986-05-02 | 1987-05-04 | Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0305399B1 (de) |
| JP (1) | JP2552691B2 (de) |
| AT (1) | ATE114334T1 (de) |
| AU (1) | AU613870B2 (de) |
| CA (1) | CA1301606C (de) |
| DE (1) | DE3750774T2 (de) |
| WO (1) | WO1987006621A1 (de) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE113665T1 (de) * | 1987-04-15 | 1994-11-15 | Yissum Res Dev Co | Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. |
| EP0481999B1 (de) * | 1989-03-10 | 1997-07-23 | VYSIS, Inc. | Interferenzverhinderung mit affinitätseinfangschemas |
| US5589335A (en) * | 1989-04-05 | 1996-12-31 | Amoco Corporation | Hybridization promotion reagents |
| AU5401290A (en) * | 1989-04-05 | 1990-11-05 | Gene-Trak Systems | Hybridization promotion reagents |
| WO1991000926A1 (en) * | 1989-07-11 | 1991-01-24 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
| US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
| US5506350A (en) * | 1990-09-20 | 1996-04-09 | Bittner; Michael L. | Production of chromosome region specific DNA sequences and transamination |
| US5459253A (en) * | 1991-07-19 | 1995-10-17 | Pharmacia P-L Biochemicals Inc. | M-RNA purification |
| EP0554437B1 (de) * | 1991-07-31 | 2000-04-05 | VYSIS, Inc. | Nukleinsäure sonden welche bei nachweis von shigellabakterien verwendbar sind |
| WO1993020234A1 (en) * | 1992-03-31 | 1993-10-14 | E.I. Du Pont De Nemours And Company | A rapid, high capacity nucleic acid based assay |
| US6001559A (en) * | 1992-07-13 | 1999-12-14 | The Pillsbury Company | Method for detecting Listeria monocytogenes via esculin hydrolysis and nucleic acid hybridization |
| CA2143428A1 (en) * | 1993-07-09 | 1995-01-19 | Takanori Oka | Method of nucleic acid-differentiation and assay kit for nucleic acid-differentiation |
| US6045996A (en) * | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| WO1997014028A2 (en) * | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| AT1082U2 (de) * | 1996-07-09 | 1996-10-25 | Labordiagnostika Ges Mbh | Verfahren zur stabilisierung von nukleinsäuren vor deren isolierung aus blutproben |
| US6361940B1 (en) | 1996-09-24 | 2002-03-26 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization and priming specificity |
| EP0958378A2 (de) * | 1996-09-24 | 1999-11-24 | Rapigene, Inc. | Zusammensetzungen und verfahren zur verbesserung der hybridisierungsspezifitaet |
| EP0952228A3 (de) * | 1996-09-24 | 1999-12-15 | Rapigene, Inc. | Zusammensetzungen und Verfahren zur Verbesserung der Hybridisierungsspezifität |
| US6210881B1 (en) | 1996-12-30 | 2001-04-03 | Becton, Dickinson And Company | Method for reducing inhibitors of nucleic acid hybridization |
| US5763185A (en) * | 1996-12-30 | 1998-06-09 | Becton Dickinson And Company | Method for reducing inhibitors of nucleic acid hybridization |
| DE19746874A1 (de) | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| DE19956820A1 (de) | 1999-11-25 | 2001-05-31 | Roche Diagnostics Gmbh | Spezies-spezifischer Nachweis von Nukleinsäuren mittels eines Analyseelementes |
| US6503716B1 (en) | 2000-11-28 | 2003-01-07 | Pe Corporation (Ny) | Compositions and methods for extracting a nucleic acid |
| US8148171B2 (en) | 2001-10-09 | 2012-04-03 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| AU2002359522A1 (en) | 2001-11-28 | 2003-06-10 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| GB0207975D0 (en) * | 2002-04-05 | 2002-05-15 | Genovision As | Isolating nucleic acid |
| DE10261529A1 (de) * | 2002-12-23 | 2004-07-08 | Indivumed Gmbh | Verfahren zur Erstellung einer Sammlung von biologischem Probenmaterial und Probensammlung |
| JP4831725B2 (ja) * | 2004-02-13 | 2011-12-07 | 栄研化学株式会社 | 簡易的核酸抽出法 |
| US20050208501A1 (en) * | 2004-03-16 | 2005-09-22 | Ambion, Inc. | Process and reagents for extraction of RNA from fractionated blood leukocytes |
| WO2010134245A1 (ja) * | 2009-05-20 | 2010-11-25 | オリンパス株式会社 | 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット |
| WO2014047141A1 (en) * | 2012-09-19 | 2014-03-27 | Beckman Coulter, Inc. | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS |
| EP4241062A4 (de) | 2020-11-11 | 2024-01-17 | George Mason Research Foundation, Inc. | Laseranheftungs-mikrodissektions-visualisierungschemie |
| KR20230169935A (ko) | 2021-01-12 | 2023-12-18 | 데피니티브 바이오테크놀로지스 엘엘씨 | 생물학적 시료들에서 핵산을 검출하기 위한 디바이스및 방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4483920A (en) * | 1982-05-17 | 1984-11-20 | Hahnemann University | Immobilization of message RNA directly from cells onto filter material |
| US4427580A (en) * | 1982-09-01 | 1984-01-24 | Cornell Research Foundation, Inc. | Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts |
-
1987
- 1987-05-01 CA CA000536166A patent/CA1301606C/en not_active Expired - Fee Related
- 1987-05-04 AT AT87903558T patent/ATE114334T1/de not_active IP Right Cessation
- 1987-05-04 AU AU74329/87A patent/AU613870B2/en not_active Ceased
- 1987-05-04 EP EP87903558A patent/EP0305399B1/de not_active Expired - Lifetime
- 1987-05-04 WO PCT/US1987/001023 patent/WO1987006621A1/en not_active Ceased
- 1987-05-04 DE DE3750774T patent/DE3750774T2/de not_active Expired - Fee Related
- 1987-05-04 JP JP62503095A patent/JP2552691B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0305399A1 (de) | 1989-03-08 |
| AU613870B2 (en) | 1991-08-15 |
| JPH01502317A (ja) | 1989-08-17 |
| WO1987006621A1 (en) | 1987-11-05 |
| CA1301606C (en) | 1992-05-26 |
| DE3750774T2 (de) | 1995-04-27 |
| EP0305399A4 (de) | 1989-12-28 |
| AU7432987A (en) | 1987-11-24 |
| JP2552691B2 (ja) | 1996-11-13 |
| EP0305399B1 (de) | 1994-11-23 |
| DE3750774D1 (de) | 1995-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |