ATE191513T1 - Verfahren zur amplifikation von nukleinsäuren - Google Patents

Verfahren zur amplifikation von nukleinsäuren

Info

Publication number
ATE191513T1
ATE191513T1 AT92901200T AT92901200T ATE191513T1 AT E191513 T1 ATE191513 T1 AT E191513T1 AT 92901200 T AT92901200 T AT 92901200T AT 92901200 T AT92901200 T AT 92901200T AT E191513 T1 ATE191513 T1 AT E191513T1
Authority
AT
Austria
Prior art keywords
amplification
nucleic acids
nucleic acid
treated
compounds
Prior art date
Application number
AT92901200T
Other languages
English (en)
Inventor
Lily Lin
Stephen T Isaacs
John E Hearst
Original Assignee
Hydrocarbon Research Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hydrocarbon Research Inc filed Critical Hydrocarbon Research Inc
Application granted granted Critical
Publication of ATE191513T1 publication Critical patent/ATE191513T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
AT92901200T 1990-11-14 1991-11-13 Verfahren zur amplifikation von nukleinsäuren ATE191513T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/614,921 US5284940A (en) 1990-11-14 1990-11-14 Preparation for nucleic acid samples
PCT/US1991/008492 WO1992008807A1 (en) 1990-11-14 1991-11-13 Preparation of nucleic acid samples

Publications (1)

Publication Number Publication Date
ATE191513T1 true ATE191513T1 (de) 2000-04-15

Family

ID=24463268

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92901200T ATE191513T1 (de) 1990-11-14 1991-11-13 Verfahren zur amplifikation von nukleinsäuren

Country Status (6)

Country Link
US (1) US5284940A (de)
EP (1) EP0557448B1 (de)
AT (1) ATE191513T1 (de)
AU (1) AU9058091A (de)
DE (1) DE69132098D1 (de)
WO (1) WO1992008807A1 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6187572B1 (en) 1990-04-16 2001-02-13 Baxter International Inc. Method of inactivation of viral and bacterial blood contaminants
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
WO1993025710A1 (en) * 1992-06-08 1993-12-23 Gen-Probe Incorporated Preparation of nucleic acid from mononuclear cells
ES2194843T3 (es) * 1992-09-11 2003-12-01 Hoffmann La Roche Deteccion de acidos nucleicos en sangre.
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
WO1995024499A1 (en) * 1994-03-10 1995-09-14 Gen-Probe Incorporated Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents
JPH10504834A (ja) * 1994-08-29 1998-05-12 アクゾ・ノベル・エヌ・ベー 核酸などの生物材料の単離に使用する装置
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
AU4586096A (en) * 1995-03-10 1996-09-19 Becton Dickinson & Company Sample processing method for whole blood
US5576197A (en) * 1995-04-07 1996-11-19 Molecular Bio-Products Polymerase chain reaction container and methods of using the same
US6605439B2 (en) 1995-08-17 2003-08-12 Eberhard-Karls Universität Tübingen Universitätsklinikum Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
AU717453B2 (en) * 1995-08-17 2000-03-23 Eberhard-Karls-Universitat Tubingen Universitatsklinikum Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
AU2117599A (en) 1998-01-20 1999-08-02 Mayo Foundation For Medical Education And Research Simplified mutation detection
US6146511A (en) * 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method
US6130042A (en) * 1998-03-05 2000-10-10 The United States Of America As Represented By The Department Of Health And Human Services Compositions and methods for diagnosing periodontal disease
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
CA2436910C (en) * 2000-12-01 2014-06-17 Response Genetics, Inc. Method of determining epidermal growth factor receptor and her2-neu gene expression and correlation of levels thereof with survival rates
US7005278B2 (en) * 2001-03-02 2006-02-28 Danenberg Kathleen D Method of determining dihydropyrimidine dehydrogenase gene expression
US7049059B2 (en) 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US20020068280A1 (en) * 2000-12-06 2002-06-06 Jeff Fairman Compositions and methods for DNA purification from whole blood
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
EP1452593B1 (de) * 2001-11-14 2009-04-08 Toyo Boseki Kabushiki Kaisha Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
ATE454469T1 (de) 2002-01-28 2010-01-15 Ambion Inc Für den nachweis von nukleinsäuren kompetente biologische rohderivate
US20030215845A1 (en) * 2002-02-19 2003-11-20 Bille Todd William Selective extraction of DNA from groups of cells
US7785822B2 (en) * 2002-08-27 2010-08-31 University Of Georgia Research Foundation, Inc. Assays for the detection of biliverdin in birds and reptiles
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
TW200521433A (en) * 2003-12-22 2005-07-01 We Gene Technologies Inc Cell detection chip and fabricating method thereof and method of detecting cell
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7939249B2 (en) * 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
CA2842232C (en) * 2005-03-10 2015-01-27 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
EP2710127A4 (de) 2011-05-20 2014-12-03 Advandx Inc Selektive ultraschall-lyse von blut und anderen biologischen flüssigkeiten und geweben
CA2954032C (en) * 2014-07-11 2021-06-01 Abbott Molecular Inc. Automated hiv-1 viral load testing procedure for dried spots
LU100795B1 (en) * 2018-05-14 2019-11-14 Diatron MI PLC Immunoassay for whole blood samples
WO2020252399A1 (en) * 2019-06-14 2020-12-17 University Of Washington An enzymatic assay to measure long-term adherence to pre exposure prophylaxis and antiretroviral therapy

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3001913A (en) * 1961-09-26 Process for preparing a nucleic acid
DE2125699B2 (de) * 1971-05-25 1973-05-17 Phywe AG, 3400 Gottingen Praeparation von zellen zur messung von dns
US3874852A (en) * 1974-07-05 1975-04-01 Coulter Diagnostics Inc Reagent and method for determining leukocytes and hemoglobin in the blood
CA1083508A (fr) * 1975-11-13 1980-08-12 Jacques Grange Supports porteurs de chaines laterales, procedes d'obtention de ces supports, procedes de fixation de composes organiques comportant un residu glucidique sur lesdits supports, produits et reactifs resultant de ladite fixation chimique
US4185964A (en) * 1977-02-08 1980-01-29 Central Laboratories of Associated Maryland Pathologists, Ltd. Lysing reagent
US4099917A (en) * 1977-07-21 1978-07-11 Technicon Instruments Corporation Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles
US4286963A (en) * 1979-11-23 1981-09-01 Coulter Electronics, Inc. Differential lymphoid-myeloid determination of leukocytes in whole blood
US4521518A (en) * 1980-06-16 1985-06-04 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4346018A (en) * 1980-06-16 1982-08-24 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4423153A (en) * 1981-12-03 1983-12-27 Board Of Regents, The University Of Texas System Methods and compositions for the detection and determination of cellular DNA
US4659678A (en) * 1982-09-29 1987-04-21 Serono Diagnostics Limited Immunoassay of antigens
US4485175A (en) * 1983-01-03 1984-11-27 Coulter Electronics, Inc. Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
JPS6073356A (ja) * 1983-09-29 1985-04-25 Toa Medical Electronics Co Ltd 血液分析用試薬
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4745071A (en) * 1985-09-05 1988-05-17 Sequoia-Turner Corporation Method for the volumetric differentiation of blood cells types
US4908318A (en) * 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US4820416A (en) * 1987-09-10 1989-04-11 The Royal Institution For The Advancement For Learning (Mcgill University) Removal of bilirubin by the pseudoperoxidase activity of immobilized hemoglobin
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids

Also Published As

Publication number Publication date
EP0557448A1 (de) 1993-09-01
US5284940A (en) 1994-02-08
DE69132098D1 (de) 2000-05-11
WO1992008807A1 (en) 1992-05-29
EP0557448A4 (en) 1997-05-21
EP0557448B1 (de) 2000-04-05
AU9058091A (en) 1992-06-11

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