ATE113665T1 - Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. - Google Patents

Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.

Info

Publication number
ATE113665T1
ATE113665T1 AT88903742T AT88903742T ATE113665T1 AT E113665 T1 ATE113665 T1 AT E113665T1 AT 88903742 T AT88903742 T AT 88903742T AT 88903742 T AT88903742 T AT 88903742T AT E113665 T1 ATE113665 T1 AT E113665T1
Authority
AT
Austria
Prior art keywords
genes
cells
expression
microcultures
rna
Prior art date
Application number
AT88903742T
Other languages
English (en)
Inventor
Raymond Kaempfer
Gila Arrad
Lisya Gerez
Original Assignee
Yissum Res Dev Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co filed Critical Yissum Res Dev Co
Application granted granted Critical
Publication of ATE113665T1 publication Critical patent/ATE113665T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT88903742T 1987-04-15 1988-04-05 Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. ATE113665T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPI145587 1987-04-15

Publications (1)

Publication Number Publication Date
ATE113665T1 true ATE113665T1 (de) 1994-11-15

Family

ID=3772116

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88903742T ATE113665T1 (de) 1987-04-15 1988-04-05 Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.

Country Status (7)

Country Link
EP (1) EP0309558B1 (de)
JP (1) JPH01503598A (de)
AT (1) ATE113665T1 (de)
DE (1) DE3852004T2 (de)
ES (1) ES2009261A6 (de)
PT (1) PT87224B (de)
WO (1) WO1988008038A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69214243T2 (de) * 1991-09-23 1997-02-06 Pfizer Verfahren für die Detektion von spezifische mRNS und DNS in Zellen
US5837467A (en) * 1997-01-21 1998-11-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Method for predicting the response to treatment with BCG of superficial bladder carcinoma
WO2001053534A2 (en) * 2000-01-21 2001-07-26 Curagen Corporation Method of identifying the function of a test agent

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
EP0124124A3 (de) * 1983-05-02 1987-08-26 Enzo Biochem, Inc. Verfahren und Materialien zur Analyse und zur Bestimmung von genetischen Substanzen
FR2552879B1 (fr) * 1983-10-03 1987-08-14 Pasteur Institut Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample

Also Published As

Publication number Publication date
EP0309558A1 (de) 1989-04-05
JPH01503598A (ja) 1989-12-07
PT87224A (pt) 1988-05-01
ES2009261A6 (es) 1989-09-16
DE3852004D1 (de) 1994-12-08
EP0309558B1 (de) 1994-11-02
PT87224B (pt) 1992-08-31
WO1988008038A1 (en) 1988-10-20
DE3852004T2 (de) 1995-06-01
EP0309558A4 (en) 1992-04-01

Similar Documents

Publication Publication Date Title
Zinker et al. The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins.
Du et al. Simultaneous determination of polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection
Nübel et al. Boronate affinity electrophoresis for the purification and analysis of cofactor-modified RNAs
ATE79474T1 (de) Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten.
Handa et al. Transcription of Simian virus 40 DNA in a HeLa whole cell extract.
Buys et al. Selective staining of the same set of nucleolar phosphoproteins by silver and Giemsa: A combined biochemical and cytochemical study on staining of NORs
ATE133995T1 (de) Verfahren zum nukleotidsequenzennachweis mittels technik der sandwich-hybridisierung
FR2422956A1 (fr) Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
DK242685A (da) Fremgangsmaade og reagenssystem til bestemmelse af en bestemt polynucleotidsekvens i et testmedium indeholdende enkeltstrengede nucleinsyrer
DK463089A (da) Fremgangsmaade og reagenssaet til detektion af en specifik nucleinsyresekvens i en proeve indeholdende en nucleinsyre
BR9507960A (pt) Sondas processos e kits para detenção de acidos nucleicos mycobacteriais em amostras biologicas
CN102373273A (zh) 一种结核分枝杆菌核酸的检测试剂盒及其方法
Pederson et al. Effect of transcription of yeast chromatin on DNA topology in vivo.
ATE113665T1 (de) Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.
Andrus et al. Polyacrylamide gel electrophoresis (PAGE) of synthetic nucleic acids
Yamada et al. Lymphocyte metallothionein-mRNA as a sensitive biomarker of cadmium exposure
Knight et al. Synthesis of ribonucleic acids during the germination of Botryodiplodia theobromae pycnidiospores
Landers et al. High-performance capillary electrophoretic analysis of chloramphenicol acetyl transferase activity
Goldstrohm et al. Regulated deadenylation in vitro
KR101137502B1 (ko) 가교결합과 가속 질량 분석법의 조합에 의한 단백질-리간드 상호작용의 확인 및 정량화 방법
Cui Sequential extraction of RNA, DNA and protein from cultured cells of the same group
Brown et al. Biosynthesis of low molecular weight RNA in mouse myeloma cells
Pater et al. Modification of methods for RNA isolation with guanidine thiocyanate and vacuum transfer to membranes improve the possibility to analyze high molecular weight transcripts from animal cells
Kaźmierczak et al. Application of capillary electrophoresis for DNA-protein binding tests
CN113740401A (zh) RNA中m5C和m6A双重分析逻辑光电化学传感方法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties