ATE79474T1 - Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten. - Google Patents

Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten.

Info

Publication number
ATE79474T1
ATE79474T1 AT85902921T AT85902921T ATE79474T1 AT E79474 T1 ATE79474 T1 AT E79474T1 AT 85902921 T AT85902921 T AT 85902921T AT 85902921 T AT85902921 T AT 85902921T AT E79474 T1 ATE79474 T1 AT E79474T1
Authority
AT
Austria
Prior art keywords
methods
unknown pathogens
detection
determination
identification
Prior art date
Application number
AT85902921T
Other languages
English (en)
Inventor
David R Scholl
Joseph D Jollick
Original Assignee
Diagnostic Research Limited Pa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnostic Research Limited Pa filed Critical Diagnostic Research Limited Pa
Application granted granted Critical
Publication of ATE79474T1 publication Critical patent/ATE79474T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/803Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT85902921T 1984-06-11 1985-06-06 Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten. ATE79474T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US06/619,286 US4652517A (en) 1984-06-11 1984-06-11 Methods for the in vitro detection and identification of unknown pathogens or genetic entities
EP85902921A EP0188450B1 (de) 1984-06-11 1985-06-06 Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten
PCT/US1985/001050 WO1986000139A1 (en) 1984-06-11 1985-06-06 Methods for the in vitro detection and identification of unknown pathogens or genetic entities

Publications (1)

Publication Number Publication Date
ATE79474T1 true ATE79474T1 (de) 1992-08-15

Family

ID=24481252

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85902921T ATE79474T1 (de) 1984-06-11 1985-06-06 Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten.

Country Status (6)

Country Link
US (1) US4652517A (de)
EP (1) EP0188450B1 (de)
JP (1) JPS61502376A (de)
AT (1) ATE79474T1 (de)
AU (1) AU594925B2 (de)
WO (1) WO1986000139A1 (de)

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US5288611A (en) * 1983-01-10 1994-02-22 Gen-Probe Incorporated Method for detecting, identifying, and quantitating organisms and viruses
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4888278A (en) * 1985-10-22 1989-12-19 University Of Massachusetts Medical Center In-situ hybridization to detect nucleic acid sequences in morphologically intact cells
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
EP0261955A3 (de) * 1986-09-26 1989-06-07 E.I. Du Pont De Nemours And Company Verfahren zur Immobilisierung von DNS
US7087742B1 (en) 1986-11-24 2006-08-08 Gen-Probe Incorporated Oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US7090972B1 (en) 1986-11-24 2006-08-15 Gen-Probe Incorporated Methods for determining the presence of non-viral organisms in a sample
US4977251A (en) * 1987-01-30 1990-12-11 University Of Illinois Board Of Trustees DNA probes for identification of bacteroides species
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
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IL85948A0 (en) * 1987-04-08 1988-09-30 Cambridge Bioscience Corp Method and kit for detecting bacteria or fungi
WO1988008036A1 (en) * 1987-04-08 1988-10-20 Invitron Corporation Detection and identification of dna and/or rna in protein-containing samples
ATE118823T1 (de) * 1987-10-06 1995-03-15 Ortho Diagnostic Systems Inc Schnelles verfahren zur identifizierung einer spezifischen sequenz von nukleinsäure.
US5294533A (en) * 1988-07-05 1994-03-15 Baylor College Of Medicine Antisense oligonucleotide antibiotics complementary to the macromolecular synthesis operon, methods of treating bacterial infections and methods for identification of bacteria
GR1002090B (en) * 1988-09-30 1995-12-28 Ortho Diagnostic Systems Inc A rapid method for identifying a specific nucleic acid sequence
US6242184B1 (en) 1988-10-18 2001-06-05 University Of Massachusetts In-situ hybridization of single-copy and multiple-copy nucleic acid sequences
US7172863B1 (en) 1988-12-09 2007-02-06 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Neisseria gonorrhoeae
EP0387696B1 (de) * 1989-03-17 1997-08-27 Abbott Laboratories Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik
DE3919393A1 (de) * 1989-06-14 1990-12-20 Hoechst Ag Verfahren zur stabilisierung von auf festphasen immobilisierten biologisch aktiven substanzen
US5106730A (en) * 1989-07-24 1992-04-21 Microprobe Corporation Lactam-containing compositions and methods useful for the hybridization of nucleic acids
US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
JPH06502305A (ja) * 1990-10-19 1994-03-17 ベクトン ディッキンソン アンド カンパニー 腔感染症に関連する微生物を検出するために有用な方法及び薬学キット
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US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
ATE307200T1 (de) * 1992-07-07 2005-11-15 Fuso Pharmaceutical Ind Sonde zur diagnose einer ansteckenden krankheit verursacht durch staphylococcus epidermidis
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5658749A (en) * 1994-04-05 1997-08-19 Corning Clinical Laboratories, Inc. Method for processing mycobacteria
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ES2110916B1 (es) * 1996-05-24 1998-10-01 Ivia Procedimiento de preparacion de dianas para la reaccion en cadena de la polimerasa (pcr).
US5985593A (en) * 1996-10-11 1999-11-16 Integrated Research Technology, L.L.C. Compositions and methods for enzymatic decontamination
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Also Published As

Publication number Publication date
AU594925B2 (en) 1990-03-22
EP0188450A1 (de) 1986-07-30
AU4431385A (en) 1986-01-10
JPH0430279B2 (de) 1992-05-21
WO1986000139A1 (en) 1986-01-03
EP0188450A4 (de) 1989-02-02
US4652517A (en) 1987-03-24
JPS61502376A (ja) 1986-10-23
EP0188450B1 (de) 1992-08-12

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