ES8605862A1 - Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad - Google Patents

Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad

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Publication number
ES8605862A1
ES8605862A1 ES539560A ES539560A ES8605862A1 ES 8605862 A1 ES8605862 A1 ES 8605862A1 ES 539560 A ES539560 A ES 539560A ES 539560 A ES539560 A ES 539560A ES 8605862 A1 ES8605862 A1 ES 8605862A1
Authority
ES
Spain
Prior art keywords
nucleic acid
characterize
detect
probe containing
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES539560A
Other languages
English (en)
Other versions
ES539560A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Publication of ES8605862A1 publication Critical patent/ES8605862A1/es
Publication of ES539560A0 publication Critical patent/ES539560A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO DE DETECCION Y CARACTERIZACION DE SECUENCIAS Y FRAGMENTOS DETERMINADOS DE ACIDOS NUCLEICOS, EN COMPOSICIONES QUE PUEDEN CONTENERLOS. COMPRENDE PONER EN CONBTACTO LA COMPOSICION CON UNA SONDA FORMADA POR UN ACIDO NUCLEICO QUE CONTIENE UNA SECUENCIA COMPLEMENTARIA DE LA SECUENCIA O FRAGMENTO DETERMINADO BUSCADO Y QUE CONTIENE AL MENOS UN GRUPO 7-YODO-N-2-ACETILAMINOFLUORENO (AAIF); SEPARAR EL EXCESO DE SONDA NO HIBRIDADA; PONER EN CONTACTO LOS ACIDOS NUCLEICOS DE LA COMPOSICION QUE RETIENEN LA SONDA EN ESTADO HIBRIDADO CON UNOS ANTICUERPOS CAPACES DE REACCIONAR CON N-2-(GUANOSINA-8-IL)-ACETILAMINOFLUORENO O CON ANTICUERPOS PREPARADOS, CONTRA UNA SECUENCIA DE ACIDO NUCLEICO MODIFICADA POR UN GRUPO N-2-ACETILAMINOFLUORENO (ANTICUERPOS AAF); Y DETECTAR LOS ANTICUERPOS ANTI-AAF FIJADOS SOBRE LOS GRUPOS AAIF DE LA SONDA HIBRIDADA. TIENE APLICACIONES PARA LA DETECCION DE UN ADN O ARN, CELULAR O VIRAL.
ES539560A 1984-01-16 1985-01-15 Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad Expired ES8605862A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8400607A FR2558172B1 (fr) 1984-01-16 1984-01-16 Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue

Publications (2)

Publication Number Publication Date
ES8605862A1 true ES8605862A1 (es) 1986-04-16
ES539560A0 ES539560A0 (es) 1986-04-16

Family

ID=9300153

Family Applications (1)

Application Number Title Priority Date Filing Date
ES539560A Expired ES8605862A1 (es) 1984-01-16 1985-01-15 Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad

Country Status (8)

Country Link
US (1) US4963477A (es)
EP (1) EP0158758B1 (es)
JP (2) JPH0740960B2 (es)
AT (1) ATE37551T1 (es)
CA (1) CA1258623A (es)
DE (1) DE3474305D1 (es)
ES (1) ES8605862A1 (es)
FR (1) FR2558172B1 (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3431536A1 (de) * 1984-08-28 1986-03-13 Boehringer Mannheim Gmbh, 6800 Mannheim Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren
FI72146C (fi) * 1985-01-02 1987-04-13 Orion Yhtymae Oy Foerfarande foer identifiering av nukleinsyror.
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
AU1070588A (en) * 1987-01-30 1988-08-04 Diagnostic Products Corporation Measuring anti-dna antibodies
US5552541A (en) * 1990-06-20 1996-09-03 Beckman Instruments, Inc. Haptenic probes for detecting capture polynucleotides
JP2649100B2 (ja) * 1990-10-30 1997-09-03 Nitsuhon Deii Pii Shii Corp 標準曲線における抗原結合率と抗二本鎖ｄｎａ抗体価の比例関係を改善するためのｓｌｅ疾患の検知法及び検知用キット
US5329461A (en) * 1992-07-23 1994-07-12 Acrogen, Inc. Digital analyte detection system
US5523208A (en) * 1994-11-30 1996-06-04 The Board Of Trustees Of The University Of Kentucky Method to discover genetic coding regions for complementary interacting proteins by scanning DNA sequence data banks
US6251591B1 (en) 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6046004A (en) * 1997-02-27 2000-04-04 Lorne Park Research, Inc. Solution hybridization of nucleic acids with antisense probes having modified backbones
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
US6656692B2 (en) 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US7220541B2 (en) * 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
CN101240329B (zh) * 2007-11-13 2010-06-23 中国科学院上海应用物理研究所 一种dna的荧光检测方法及其试剂盒
EP2511380B1 (en) * 2011-04-15 2013-12-25 Diagenode S.A. Method and apparatus for fragmenting DNA sequences
US20160187325A1 (en) * 2013-08-21 2016-06-30 Fujirebio Inc. Method and kit for measuring modified nucleobase using heterogeneous nucleic acid probe

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
FR2518755B1 (fr) * 1981-12-23 1986-04-11 Pasteur Institut Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
EP0128018B1 (en) * 1983-05-31 1992-07-15 Orgenics Ltd. Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
US4623627A (en) * 1983-08-19 1986-11-18 Cetus Corporation Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same

Also Published As

Publication number Publication date
JPS60231693A (ja) 1985-11-18
US4963477A (en) 1990-10-16
DE3474305D1 (en) 1988-11-03
ATE37551T1 (de) 1988-10-15
EP0158758B1 (fr) 1988-09-28
EP0158758A1 (fr) 1985-10-23
JPH07246100A (ja) 1995-09-26
FR2558172B1 (fr) 1986-06-13
ES539560A0 (es) 1986-04-16
CA1258623A (en) 1989-08-22
JPH0740960B2 (ja) 1995-05-10
FR2558172A1 (fr) 1985-07-19

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FD1A Patent lapsed

Effective date: 19970519