DE69632466D1 - Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen - Google Patents

Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen

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Publication number
DE69632466D1
DE69632466D1 DE69632466T DE69632466T DE69632466D1 DE 69632466 D1 DE69632466 D1 DE 69632466D1 DE 69632466 T DE69632466 T DE 69632466T DE 69632466 T DE69632466 T DE 69632466T DE 69632466 D1 DE69632466 D1 DE 69632466D1
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Germany
Prior art keywords
solution
proteins
methods
rna
chaotropic agent
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Expired - Lifetime
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DE69632466T
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English (en)
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DE69632466T3 (de
DE69632466T2 (de
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Piotr Chomczynski
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Publication of DE69632466T2 publication Critical patent/DE69632466T2/de
Publication of DE69632466T3 publication Critical patent/DE69632466T3/de
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Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69632466T 1995-07-31 1996-07-18 Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen Expired - Lifetime DE69632466T3 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US509164 1995-07-31
US08/509,164 US5945515A (en) 1995-07-31 1995-07-31 Product and process for isolating DNA, RNA and proteins
PCT/US1996/011875 WO1997005248A2 (en) 1995-07-31 1996-07-18 Solution containing chaotropic agent and process using it for isolation of dna, rna and proteins
EP96925355A EP0843724B2 (de) 1995-07-31 1996-07-18 Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen

Publications (3)

Publication Number Publication Date
DE69632466D1 true DE69632466D1 (de) 2004-06-17
DE69632466T2 DE69632466T2 (de) 2005-05-12
DE69632466T3 DE69632466T3 (de) 2012-05-24

Family

ID=24025561

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69632466T Expired - Lifetime DE69632466T3 (de) 1995-07-31 1996-07-18 Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen

Country Status (6)

Country Link
US (1) US5945515A (de)
EP (1) EP0843724B2 (de)
AT (1) ATE266723T1 (de)
AU (1) AU6548096A (de)
DE (1) DE69632466T3 (de)
WO (1) WO1997005248A2 (de)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
US6326147B1 (en) 1999-05-13 2001-12-04 The Perkin-Elmer Corporation Methods, apparatus, articles of manufacture, and user interfaces for performing automated biological assay preparation and macromolecule purification
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6936414B2 (en) * 1999-12-22 2005-08-30 Abbott Laboratories Nucleic acid isolation method and kit
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US6521411B2 (en) 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
KR20020029477A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의한 유전자 추출방법
KR20020029476A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의해 유전자를 추출하기 위한 라이시스버퍼시약
KR20100101709A (ko) * 2000-12-01 2010-09-17 리스판스 지네틱스, 인크. 표피 성장 인자 수용체 및 her2-neu 유전자 발현 및 이들 수준과 생존율의 상관성을 측정하는 방법
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US7005278B2 (en) * 2001-03-02 2006-02-28 Danenberg Kathleen D Method of determining dihydropyrimidine dehydrogenase gene expression
KR100426544B1 (ko) * 2000-12-08 2004-04-13 바이오코아 주식회사 혈청 또는 혈장으로부터 바이러스의 핵산을 추출하는 방법및 그 방법에 사용되는 핵산 추출용 조성물
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
KR100454869B1 (ko) * 2001-07-13 2004-11-03 주식회사 인트론바이오테크놀로지 Dna 추출용 세포 용해 버퍼 및 이를 이용한 dna 추출방법
AU2002325617A1 (en) * 2001-08-29 2003-03-10 Hexal Pharma Gmbh Method and device for preparing a sample of biological origin in order to determine at least one constituent contained therein
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7208271B2 (en) 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7482116B2 (en) * 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
DE602004026854D1 (de) 2003-03-19 2010-06-10 Univ British Columbia Als indikatoren zur patientenprognose geeignete haplotypen von plasminogenaktivator-inhibitor-1 (pai-1)
WO2004086496A1 (ja) * 2003-03-26 2004-10-07 Shin-Etsu Handotai Co., Ltd. 熱処理用ウェーハ支持具及び熱処理装置
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US7488579B2 (en) * 2003-05-06 2009-02-10 Biochain Institute, Inc. Methods and compositions to extract DNA, RNA and protein simultaneously from biological samples
JP4836795B2 (ja) * 2003-05-19 2011-12-14 ブランデイズ ユニバーシティー 核酸プロセシング方法、キット、及び装置
EP1633869A1 (de) * 2003-06-04 2006-03-15 Qiagen AS Verfahren zur sequenziellen isolierung von dns und rns aus derselben nukleinsäure-enthaltenden probe
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
ATE466080T1 (de) * 2003-12-16 2010-05-15 Qiagen North American Holdings Formulierungen und verfahren zum denaturieren von proteinen
ITMI20040167A1 (it) * 2004-02-04 2004-05-04 Univ Padova Metodo per l'estrazione simultanea di acidi nucleici da un campione biologico
EP1584923A3 (de) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilisierung von Biomolekülen in Proben
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2005113147A2 (en) * 2004-04-08 2005-12-01 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
AU2006249235B2 (en) * 2004-05-14 2010-11-11 Abraxis Bioscience, Llc Sparc and methods of use thereof
US8420603B2 (en) * 2004-05-14 2013-04-16 Abraxis Bioscience, Llc SPARC and methods of use thereof
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
US20060272078A1 (en) * 2004-10-29 2006-12-07 Riccardo Polinelli Apparatus and methodology to mitigate fogging on dual lens sports goggle
CN101124321B (zh) 2004-11-05 2012-02-29 恰根北美控股有限公司 用于从稳定剂中纯化核酸的组合物和方法
DK1817423T3 (en) 2004-11-30 2015-01-26 Merial Ltd Mixing units for chemical light ring of cells
US7727718B2 (en) * 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
WO2006112930A2 (en) * 2005-02-18 2006-10-26 Abraxis Bioscience, Inc. Q3 sparc deletion mutant and uses thereof
JP2008532528A (ja) 2005-03-16 2008-08-21 ディーエヌエー ジェノテック インク 体液から得た核酸を保存するための組成物および方法
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
US8628918B2 (en) * 2005-05-09 2014-01-14 Affymetrix, Inc. Multiplex capture of nucleic acids
WO2006124771A2 (en) 2005-05-12 2006-11-23 Panomics, Inc. Multiplex branched-chain dna assays
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
KR100622606B1 (ko) 2005-06-16 2006-09-11 (주)엘피스바이오텍 단일공정에 의한 플라스미드 dna 정제용 조성물과 그를이용하여 플라스미드 dna를 정제하는 방법
CN104673905B (zh) * 2005-06-20 2016-09-07 领先细胞医疗诊断有限公司 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法
WO2006136033A1 (en) * 2005-06-23 2006-12-28 The University Of British Columbia Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
JP5150507B2 (ja) 2005-12-09 2013-02-20 ディーエヌエー ジェノテック インク 物質を解放できるように貯蔵するための容器システム
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US7687254B2 (en) * 2006-07-11 2010-03-30 Case Western Reserve University Phenol-free method of isolating DNA
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8652782B2 (en) 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
EP1932913B1 (de) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nukleinsäureisolation mithilfe von Polidocanol und Derivaten davon
CN102776173B (zh) 2006-12-11 2014-10-29 霍夫曼-拉罗奇有限公司 使用聚多卡醇和衍生物的核酸分离
US20080255035A1 (en) * 2007-04-13 2008-10-16 Abraxis Bioscience, Inc. Sparc and methods of use thereof
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
EP3058954B1 (de) * 2007-08-27 2017-03-01 Longhorn Vaccines and Diagnostics, LLC Immunogene zusammensetzungen und verfahren
US20110300608A1 (en) * 2007-09-21 2011-12-08 Streck, Inc. Nucleic acid isolation in preserved whole blood
AU2012211365B9 (en) * 2007-10-01 2014-05-15 Longhorn Vaccines & Diagnostics, Llc Biological specimen collection and transport system and methods of use
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
AU2008343745B2 (en) * 2007-10-01 2012-05-10 Longhorn Vaccines & Diagnostics Llc Biological specimen collection and transport system and methods of use
EP2212437A4 (de) * 2007-11-07 2011-09-28 Univ British Columbia Mikrofluidische vorrichtung und verfahren zu ihrer anwendung
US20090143570A1 (en) * 2007-11-30 2009-06-04 Ge Healthcare Bio-Sciences Corp. Method for isolation of genomic dna, rna and proteins from a single sample
WO2010033652A1 (en) * 2008-09-17 2010-03-25 Ge Healthcare Bio-Sciences Corp. Method for small rna isolation
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
ES2543367T3 (es) 2010-07-07 2015-08-18 Diagon Kft. Procedimiento para el aislamiento específico del contenido de ADN total de gérmenes bacterianos y un kit para dicho fin
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
DK2607482T3 (en) * 2010-08-18 2017-07-03 Toray Industries Solution for extraction of RNA
ES2562821T3 (es) 2010-10-21 2016-03-08 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof
US8530228B2 (en) * 2010-12-28 2013-09-10 Bexmart Integrated versatile and systems preparation of specimens
CN102250876B (zh) * 2011-05-18 2014-08-13 李学敬 从生物材料中分离纯化rna的方法
CN103890163B (zh) 2011-06-19 2016-09-14 阿博根公司 用于样品采集的装置、溶液和方法
EP2773390B1 (de) 2011-11-03 2020-12-30 Tripath Imaging, Inc. Verfahren und zusammensetzungen zur vorbereitung von proben auf die immunoeinfärbung
CA2863083C (en) 2012-01-26 2023-09-19 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
AU2013240200B2 (en) * 2012-03-28 2016-10-20 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
US9540635B2 (en) 2012-05-09 2017-01-10 Bio-Rad Laboratories, Inc. Buffer for one-step DNA extraction
CN102876663B (zh) * 2012-10-10 2014-07-30 北京百泰克生物技术有限公司 一种从液体样本中快速提取总rna的试剂及方法
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
JP5782533B2 (ja) * 2013-03-28 2015-09-24 富士フイルム株式会社 クロマトグラフ方法及びクロマトグラフキット
EP3656215B1 (de) 2014-06-10 2021-08-04 Biomatrica, INC. Stabilisierung von thrombozyten bei umgebungstemperatur
WO2016064887A1 (en) 2014-10-20 2016-04-28 Gen-Probe Incorporated Red blood cell lysis solution
GB2531741A (en) * 2014-10-28 2016-05-04 Bisn Laboratory Services Ltd Molecular and bioinformatics methods for direct sequencing
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
GB2538955A (en) * 2015-05-29 2016-12-07 Ge Healthcare Uk Ltd Improvements in and relating to extracting biological analytes
US11078528B2 (en) 2015-10-12 2021-08-03 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
KR20230145258A (ko) 2015-12-08 2023-10-17 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
US11427815B2 (en) * 2015-12-28 2022-08-30 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
JP7134869B2 (ja) 2016-04-27 2022-09-12 ジェン-プローブ・インコーポレーテッド 溶血試薬
CA3043489A1 (en) 2016-11-21 2018-05-24 Nanostring Technologies, Inc. Chemical compositions and methods of using same
MX2019006767A (es) 2017-01-16 2019-08-22 Spectrum Solutions L L C Solucion de conservacion de acido nucleico y metodos de elaboracion y uso.
WO2018169754A1 (en) 2017-03-17 2018-09-20 Syngenta Participations Ag Methods and compositions for preserving tissues and nucleic acids
CA3099909A1 (en) 2018-05-14 2019-11-21 Nanostring Technologies, Inc. Chemical compositions and methods of using same
KR20210118065A (ko) 2018-11-20 2021-09-29 스펙트럼 솔루션즈 엘엘씨 밀봉 캡 및 밸브를 포함하는 샘플 수집 시스템
CN111269284B (zh) * 2018-12-04 2023-07-25 中国科学院大连化学物理研究所 同步分离细胞质和细胞核中蛋白质和rna的试剂及方法
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3147185A (en) * 1959-09-10 1964-09-01 Merck & Co Inc Process for purifying viral substances and composition
US3389133A (en) * 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
DE69130394T2 (de) * 1990-07-13 1999-04-08 Microprobe Corp., Bothell, Wash. Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren
US5130423A (en) * 1990-07-13 1992-07-14 Microprobe Corporation Non-corrosive compositions and methods useful for the extraction of nucleic acids
EP0544034A1 (de) * 1991-11-28 1993-06-02 Siemens Aktiengesellschaft Verfahren zur Wiederherstellung der Reihenfolge von Nachrichtenzellen
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide

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EP0843724A2 (de) 1998-05-27
DE69632466T3 (de) 2012-05-24
EP0843724B1 (de) 2004-05-12
ATE266723T1 (de) 2004-05-15
DE69632466T2 (de) 2005-05-12
AU6548096A (en) 1997-02-26
WO1997005248A2 (en) 1997-02-13
WO1997005248A3 (en) 1997-03-06
US5945515A (en) 1999-08-31
EP0843724B2 (de) 2011-07-20

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