DE3852004T2 - Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. - Google Patents

Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.

Info

Publication number
DE3852004T2
DE3852004T2 DE3852004T DE3852004T DE3852004T2 DE 3852004 T2 DE3852004 T2 DE 3852004T2 DE 3852004 T DE3852004 T DE 3852004T DE 3852004 T DE3852004 T DE 3852004T DE 3852004 T2 DE3852004 T2 DE 3852004T2
Authority
DE
Germany
Prior art keywords
genes
cells
expression
microcultures
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3852004T
Other languages
English (en)
Other versions
DE3852004D1 (de
Inventor
Raymond Kaempfer
Gila Arrad
Lisya Gerez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of DE3852004D1 publication Critical patent/DE3852004D1/de
Application granted granted Critical
Publication of DE3852004T2 publication Critical patent/DE3852004T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
DE3852004T 1987-04-15 1988-04-05 Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen. Expired - Fee Related DE3852004T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPI145587 1987-04-15
PCT/AU1988/000099 WO1988008038A1 (en) 1987-04-15 1988-04-05 Method for detecting the expression of bioregulatory genes in microcultures of eukaryotic cells and kit therefor

Publications (2)

Publication Number Publication Date
DE3852004D1 DE3852004D1 (de) 1994-12-08
DE3852004T2 true DE3852004T2 (de) 1995-06-01

Family

ID=3772116

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3852004T Expired - Fee Related DE3852004T2 (de) 1987-04-15 1988-04-05 Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.

Country Status (7)

Country Link
EP (1) EP0309558B1 (de)
JP (1) JPH01503598A (de)
AT (1) ATE113665T1 (de)
DE (1) DE3852004T2 (de)
ES (1) ES2009261A6 (de)
PT (1) PT87224B (de)
WO (1) WO1988008038A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534640B1 (de) * 1991-09-23 1996-10-02 Pfizer Inc. Verfahren für die Detektion von spezifische mRNS und DNS in Zellen
US5837467A (en) * 1997-01-21 1998-11-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Method for predicting the response to treatment with BCG of superficial bladder carcinoma
AU2001229646A1 (en) * 2000-01-21 2001-07-31 Curagen Corporation Method of identifying the function of a test agent

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
ES8600404A1 (es) * 1983-05-02 1985-10-01 Enzo Biochem Inc Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
FR2552879B1 (fr) * 1983-10-03 1987-08-14 Pasteur Institut Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample

Also Published As

Publication number Publication date
DE3852004D1 (de) 1994-12-08
JPH01503598A (ja) 1989-12-07
EP0309558A1 (de) 1989-04-05
PT87224A (pt) 1988-05-01
ES2009261A6 (es) 1989-09-16
PT87224B (pt) 1992-08-31
EP0309558B1 (de) 1994-11-02
WO1988008038A1 (en) 1988-10-20
ATE113665T1 (de) 1994-11-15
EP0309558A4 (en) 1992-04-01

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DE3852004T2 (de) Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen.
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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee