DE69702333D1 - Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung - Google Patents

Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung

Info

Publication number
DE69702333D1
DE69702333D1 DE69702333T DE69702333T DE69702333D1 DE 69702333 D1 DE69702333 D1 DE 69702333D1 DE 69702333 T DE69702333 T DE 69702333T DE 69702333 T DE69702333 T DE 69702333T DE 69702333 D1 DE69702333 D1 DE 69702333D1
Authority
DE
Germany
Prior art keywords
dna polymerase
sequencing
thermostable dna
decoupled
direct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69702333T
Other languages
English (en)
Other versions
DE69702333T2 (de
Inventor
Svante Erik Prof Dr Paeaebo
Christian Alexis Dr Kilger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE69702333T priority Critical patent/DE69702333T2/de
Publication of DE69702333D1 publication Critical patent/DE69702333D1/de
Application granted granted Critical
Publication of DE69702333T2 publication Critical patent/DE69702333T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
DE69702333T 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung Expired - Fee Related DE69702333T2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE69702333T DE69702333T2 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19653494A DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
DE69702333T DE69702333T2 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung

Publications (2)

Publication Number Publication Date
DE69702333D1 true DE69702333D1 (de) 2000-07-27
DE69702333T2 DE69702333T2 (de) 2000-12-14

Family

ID=7815667

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19653494A Ceased DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
DE69702333T Expired - Fee Related DE69702333T2 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19653494A Ceased DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung

Country Status (5)

Country Link
US (3) US6225092B1 (de)
EP (1) EP0854196B1 (de)
JP (1) JPH10225297A (de)
AT (1) ATE194015T1 (de)
DE (2) DE19653494A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4065397A (en) 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
AU743025B2 (en) * 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6605434B1 (en) * 1999-03-16 2003-08-12 Human Genome Sciences, Inc. Direct bacterial lysate sequencing
EP1144689A1 (de) * 1999-11-26 2001-10-17 Bioneer Corporation; Dna sequenzierungsmethode die verschiedene dna polymerasen verwendet und entsprechender kit
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6436641B1 (en) * 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20030219770A1 (en) * 2001-11-08 2003-11-27 Eshleman James R. Methods and systems of nucleic acid sequencing
WO2003048308A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
AU2003213107A1 (en) * 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
WO2005019476A1 (en) * 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
EP1634965B1 (de) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Echtzeit-PCR unter Zusatz von Pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2010135384A2 (en) 2009-05-21 2010-11-25 Siemens Healthcare Diagnostics Inc. Universal tags with non-natural nucleobases
US9617588B2 (en) * 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
JP6356694B2 (ja) 2012-12-13 2018-07-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
CA2114124A1 (en) * 1991-07-24 1993-02-04 Keith Gregg Single step amplification and sequencing of nucleic acids
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification

Also Published As

Publication number Publication date
US20020177129A1 (en) 2002-11-28
JPH10225297A (ja) 1998-08-25
US20040146911A1 (en) 2004-07-29
ATE194015T1 (de) 2000-07-15
DE19653494A1 (de) 1998-06-25
US6225092B1 (en) 2001-05-01
EP0854196A1 (de) 1998-07-22
DE69702333T2 (de) 2000-12-14
EP0854196B1 (de) 2000-06-21

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