ATE557083T1 - Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität - Google Patents

Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität

Info

Publication number
ATE557083T1
ATE557083T1 AT10172676T AT10172676T ATE557083T1 AT E557083 T1 ATE557083 T1 AT E557083T1 AT 10172676 T AT10172676 T AT 10172676T AT 10172676 T AT10172676 T AT 10172676T AT E557083 T1 ATE557083 T1 AT E557083T1
Authority
AT
Austria
Prior art keywords
thermostable
attenuated
dna polymerase
exonuclease activity
thermoactive dna
Prior art date
Application number
AT10172676T
Other languages
English (en)
Inventor
Nancy J Schoenbrunner
Thomas W Myers
David H Gelfand
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE557083T1 publication Critical patent/ATE557083T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT10172676T 2002-04-02 2003-03-31 Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität ATE557083T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36981502P 2002-04-02 2002-04-02

Publications (1)

Publication Number Publication Date
ATE557083T1 true ATE557083T1 (de) 2012-05-15

Family

ID=28042066

Family Applications (1)

Application Number Title Priority Date Filing Date
AT10172676T ATE557083T1 (de) 2002-04-02 2003-03-31 Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität

Country Status (6)

Country Link
US (1) US7148049B2 (de)
EP (2) EP1350841B1 (de)
JP (2) JP4034684B2 (de)
CN (1) CN1482240B (de)
AT (1) ATE557083T1 (de)
ES (1) ES2389711T3 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
JP2007159401A (ja) * 2004-05-07 2007-06-28 Precision System Science Co Ltd 改変タンパク質の作製方法
EP2292767B1 (de) * 2004-06-04 2013-11-20 Takara Bio, Inc. Polypeptide mit DNA-Polymeraseaktivität
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
US7900380B2 (en) * 2005-10-13 2011-03-08 Masterfit Enterprises Inc. User moldable adjustable insert
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
WO2009068268A1 (en) * 2007-11-28 2009-06-04 Roche Diagnostics Gmbh Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
MX340406B (es) * 2009-01-15 2016-07-07 Nat Univ Corp Univ Of Toyama Preparacion enzimatica que contiene polimerasa de adn termoestable, metodo para su produccion, y metodo para detectar un organismo objeto.
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
EP2582807B1 (de) 2010-06-18 2015-03-18 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
WO2011157433A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
US8735121B2 (en) 2010-06-18 2014-05-27 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-match discrimination
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
JP5876478B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US8722378B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
WO2012110061A1 (en) 2011-02-15 2012-08-23 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
WO2012162621A1 (en) * 2011-05-26 2012-11-29 Brandeis University Methods for suppression pcr
WO2013013822A1 (en) 2011-07-28 2013-01-31 Roche Diagnostics Gmbh Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2788479B1 (de) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
WO2015026845A2 (en) 2013-08-19 2015-02-26 Abbott Molecular Inc. Nucleotide analogs
CN104152426A (zh) * 2014-08-05 2014-11-19 孙启明 一种t5核酸外切酶在大肠杆菌中的制备方法
CN104313132A (zh) * 2014-09-28 2015-01-28 南京诺唯赞生物科技有限公司 一种t4 dna聚合酶活性测定方法
GB2542391A (en) * 2015-09-17 2017-03-22 Annexin Pharmaceuticals Ab Process of manufacture
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素
AU2017386515B2 (en) * 2016-12-30 2021-08-19 Pacific Biosciences Of California, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018148726A1 (en) * 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
CN111542532B (zh) * 2017-10-04 2024-03-15 生捷科技控股公司 酶法合成寡核苷酸的方法和体系
TWI680168B (zh) * 2017-10-18 2019-12-21 環球晶圓股份有限公司 碳化矽晶片
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
JP7218558B2 (ja) * 2017-12-19 2023-02-07 東ソー株式会社 核酸増幅法および核酸増幅試薬
EP3768832B1 (de) 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna-polymerasen zum effizienten und effektiven einbau von methylierten dntps
WO2020053327A1 (en) 2018-09-13 2020-03-19 F. Hoffmann-La Roche Ag Mutant dna polymerase(s) with improved strand displacement ability

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK149867C (da) 1979-08-08 1987-04-06 New Zealand Dev Finance Proteolytisk enzympraeparat og fremgangsmaade til fremstilling deraf
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4419446A (en) 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4601978A (en) 1982-11-24 1986-07-22 The Regents Of The University Of California Mammalian metallothionein promoter system
US4711845A (en) 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette
US4666848A (en) 1984-08-31 1987-05-19 Cetus Corporation Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element
EP0196864A3 (de) 1985-03-25 1988-03-23 Cetus Corporation Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5352600A (en) 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US5352778A (en) 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5210036A (en) 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5322785A (en) 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
DE69132477T2 (de) 1990-09-28 2001-06-13 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6100078A (en) 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
CN1123328A (zh) 1994-11-17 1996-05-29 中国科学院上海生物化学研究所 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst)
US5834253A (en) 1994-11-17 1998-11-10 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity
US5602011A (en) 1995-01-18 1997-02-11 Pharmacia Biotech Inc. Purified Thermococcus barossii DNA polymerase
US6001645A (en) 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US5830714A (en) 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
CZ293215B6 (cs) 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
SG86311A1 (en) 1997-04-10 2002-02-19 Univ Singapore Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity
EP0997530B1 (de) * 1997-06-26 2006-08-23 Takara Bio Inc. Dna polymerase-verwandte faktoren
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
US5988614A (en) 1997-09-25 1999-11-23 Atro Engineered Sytems, Inc. Bushing for heavy vehicles
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6214557B1 (en) 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases

Also Published As

Publication number Publication date
US7148049B2 (en) 2006-12-12
EP2272952A1 (de) 2011-01-12
ES2389711T3 (es) 2012-10-30
EP1350841A3 (de) 2003-12-10
CN1482240B (zh) 2012-09-05
EP1350841B1 (de) 2015-05-06
EP2272952B8 (de) 2012-08-15
EP2272952B1 (de) 2012-05-09
US20040005599A1 (en) 2004-01-08
JP4034684B2 (ja) 2008-01-16
JP2003304870A (ja) 2003-10-28
JP4585996B2 (ja) 2010-11-24
EP1350841A2 (de) 2003-10-08
CN1482240A (zh) 2004-03-17
JP2006311867A (ja) 2006-11-16

Similar Documents

Publication Publication Date Title
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE198083T1 (de) Thermostable nukleinsäure polymerase
ATE360093T1 (de) Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure
DK1705253T3 (da) Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)
DE69524653T2 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
ATE427361T1 (de) Verfahren zur dna-amplifikation und - sequenzierung
ATE400663T1 (de) Rekombinase-polymerase-amplifikation
CA2201885A1 (en) Dna polymerases having modified nucleotide binding site for dna sequencing
BR0101493B1 (pt) mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes.
DE60235491D1 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
ATE364718T1 (de) Verfahren zur vervielfältigung von nukleinsäure
DE60112746D1 (de) Kälteempfindliche dna-polymerasemutanten
EP2314596A3 (de) DNA-Polymerase-mischungen und mutante DNA-polymerasen
WO2006030455A8 (en) Dna polymerases having strand displacement activity
CA2127188A1 (en) Reagents and Methods for Coupled High Temperature Reverse Transcription and Polymerase Chain Reactions
ATE455868T1 (de) Polymerase-kettenreaktionsverfahren unter einsatz einer dna-polymerase mit proofreading- eigenschaften
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
ATE346937T1 (de) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
DE602005023586D1 (de) Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'-nichttranslatierten bereichs von hcv
DE60024464D1 (de) Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren