ATE400663T1 - Rekombinase-polymerase-amplifikation - Google Patents
Rekombinase-polymerase-amplifikationInfo
- Publication number
- ATE400663T1 ATE400663T1 AT03711216T AT03711216T ATE400663T1 AT E400663 T1 ATE400663 T1 AT E400663T1 AT 03711216 T AT03711216 T AT 03711216T AT 03711216 T AT03711216 T AT 03711216T AT E400663 T1 ATE400663 T1 AT E400663T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- recombinase
- amplification
- polymerase amplification
- polymerase
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
- G06F16/90—Details of database functions independent of the retrieved data types
- G06F16/95—Retrieval from the web
- G06F16/951—Indexing; Web crawling techniques
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
- G06F16/90—Details of database functions independent of the retrieved data types
- G06F16/95—Retrieval from the web
- G06F16/957—Browsing optimisation, e.g. caching or content distillation
- G06F16/9574—Browsing optimisation, e.g. caching or content distillation of access to content, e.g. by caching
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35856302P | 2002-02-21 | 2002-02-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE400663T1 true ATE400663T1 (de) | 2008-07-15 |
Family
ID=27765964
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03711216T ATE400663T1 (de) | 2002-02-21 | 2003-02-21 | Rekombinase-polymerase-amplifikation |
Country Status (11)
Country | Link |
---|---|
US (7) | US7270981B2 (de) |
EP (4) | EP1992706B1 (de) |
JP (4) | JP4340543B2 (de) |
AT (1) | ATE400663T1 (de) |
AU (1) | AU2003215391B2 (de) |
CA (1) | CA2476481C (de) |
DE (1) | DE60322044D1 (de) |
DK (1) | DK1499738T3 (de) |
ES (1) | ES2310237T3 (de) |
PT (1) | PT1499738E (de) |
WO (1) | WO2003072805A2 (de) |
Families Citing this family (150)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE397095T1 (de) | 2001-04-20 | 2008-06-15 | Penn State Res Found | Verfahren zur manipulation von nukleinsäuren |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
CA2498764C (en) * | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
US20040241732A1 (en) * | 2003-04-11 | 2004-12-02 | Chu-An Chang | Method of generating long nucleic acid molecules of defined sequence |
JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
JP5026958B2 (ja) * | 2004-06-01 | 2012-09-19 | アリーア サン ディエゴ, インコーポレイテッド | リコンビナーゼポリメラーゼ増幅 |
AU2011202896B2 (en) * | 2004-06-01 | 2012-12-06 | Abbott Diagnostics Scarborough, Inc. | Recombinase Polymerase Amplification |
ATE407939T1 (de) | 2004-06-02 | 2008-09-15 | Asm Scient Inc | 2ß-NITROBENZYL-MODIFIZIERTE RIBONUKLEOTIDE |
JP4534138B2 (ja) * | 2004-12-21 | 2010-09-01 | アイシン精機株式会社 | 核酸増幅反応における高度好熱菌由来タンパク質の活性化方法およびその利用 |
US7579153B2 (en) * | 2005-01-25 | 2009-08-25 | Population Genetics Technologies, Ltd. | Isothermal DNA amplification |
EP1929049B1 (de) * | 2005-07-25 | 2013-04-10 | Alere San Diego, Inc. | Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung |
WO2007087262A2 (en) * | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Selective genome amplification |
AU2007298650B2 (en) | 2006-05-04 | 2013-10-17 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
CN103203256B (zh) | 2006-07-19 | 2015-09-23 | 博纳基因技术有限公司 | 纳米口装置阵列:它们的制备以及在大分子分析中的应用 |
EP2604344A3 (de) | 2007-03-28 | 2014-07-16 | BioNano Genomics, Inc. | Verfahren zur makromolekularen Analyse mittels Nanokanalanordnungen |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
EP2318547B1 (de) | 2008-06-30 | 2018-05-30 | BioNano Genomics, Inc. | Verfahren zur einzelmolekül-gesamtgenomanalyse |
WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
US9181578B2 (en) | 2008-11-18 | 2015-11-10 | Bionano Genomics, Inc. | Polynucleotide mapping and sequencing |
SG172965A1 (en) | 2009-01-13 | 2011-08-29 | Fluidigm Corp | Single-cell nucleic acid analysis |
US8685648B2 (en) | 2009-02-03 | 2014-04-01 | Biohelix Corporation | Endonuclease-enhanced helicase-dependent amplification |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質 |
US20100299773A1 (en) * | 2009-05-20 | 2010-11-25 | Monsanto Technology Llc | Methods and compositions for selecting an improved plant |
WO2010141940A1 (en) * | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
WO2010147804A1 (en) * | 2009-06-10 | 2010-12-23 | Wisconsin Alumni Research Foundation | Method for dna double-strand break repair in vitro and applications thereof |
NO329699B1 (no) * | 2009-06-16 | 2010-12-06 | Agr Cannseal As | Brønnverktøy og fremgangsmåte for in situ innføring av et behandlingsfluid i et ringrom i en brønn |
WO2011047223A1 (en) | 2009-10-16 | 2011-04-21 | Monsanto Technology Llc | Methods of polynucleotide detection |
GB0922097D0 (en) | 2009-12-17 | 2010-02-03 | Atlas Genetics Ltd | Microbial assay |
US8759037B2 (en) | 2010-02-23 | 2014-06-24 | Illumina Cambridge Limited | Amplification methods to minimise sequence specific bias |
US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
EP2625526B1 (de) | 2010-10-04 | 2017-03-15 | Genapsys Inc. | Systeme und verfahren für automatisierte und wiederverwendbare parallele biologische reaktionen |
BR112013009001A2 (pt) | 2010-10-12 | 2016-07-05 | Monsanto Technology Llc | planta e semente de soja correspondendo a evento transgênico mon87712 e métodos para deteção das mesmas |
EP3564392B1 (de) | 2010-12-17 | 2021-11-24 | Life Technologies Corporation | Verfahren für nukleinsäureamplifikation |
JP5932832B2 (ja) | 2010-12-17 | 2016-06-08 | モンサント テクノロジー エルエルシー | 植物細胞の適格性を改善する方法 |
CA2831140C (en) | 2011-04-07 | 2017-11-07 | Alere San Diego, Inc. | Monitoring recombinase polymerase amplification mixtures |
WO2012149193A2 (en) | 2011-04-29 | 2012-11-01 | Monsanto Technology Llc | Diagnostic molecular markers for seed lot purity traits in soybeans |
US9476040B2 (en) | 2011-05-02 | 2016-10-25 | Board Of Regents Of The University Of Nebraska | Plants with useful traits and related methods |
EP2714935B1 (de) | 2011-05-27 | 2017-03-15 | Genapsys Inc. | Systeme und verfahren für genetische und biologische analysen |
US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
US9701976B2 (en) | 2011-06-30 | 2017-07-11 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof |
WO2013012440A2 (en) * | 2011-07-21 | 2013-01-24 | Cornell University | Methods and devices for dna sequencing and molecular diagnostics |
PT3517615T (pt) | 2011-08-31 | 2022-08-09 | Seminis Vegetable Seeds Inc | Métodos e composições para a firmeza da melancia |
US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
EP2785868B1 (de) | 2011-12-01 | 2017-04-12 | Genapsys Inc. | Systeme und verfahren für hocheffiziente elektronische sequenzierung und nachweis |
JP5958034B2 (ja) * | 2012-04-11 | 2016-07-27 | 富士レビオ株式会社 | 分子ビーコン型プローブを用いた標的核酸の検出方法 |
CN114854832A (zh) | 2012-04-19 | 2022-08-05 | 生命技术公司 | 核酸扩增 |
EP3461910B1 (de) | 2012-04-19 | 2020-08-26 | Life Technologies Corporation | Nukleinsäureamplifikation |
US10927393B2 (en) | 2012-06-08 | 2021-02-23 | Ionian Technologies, Llc | Nucleic acid amplifications |
DE102012014981B3 (de) * | 2012-07-26 | 2013-07-18 | Sartorius Stedim Biotech Gmbh | Verfahren zur Differenzierung zwischen lebenden und toten Zellen |
WO2014031163A1 (en) | 2012-08-24 | 2014-02-27 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing |
EP2895620B1 (de) | 2012-09-11 | 2017-08-02 | Life Technologies Corporation | Nukleinsäureamplifikation |
WO2014043143A1 (en) | 2012-09-11 | 2014-03-20 | Life Technologies Corporation | Nucleic acid amplification |
US10314253B2 (en) | 2012-12-04 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and compositions for watermelon sex expression |
CA2903874C (en) | 2013-03-15 | 2024-02-20 | Theranos, Inc. | Nucleic acid amplification |
US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
CA2906805A1 (en) | 2013-03-15 | 2014-09-18 | Theranos, Inc. | Nucleic acid amplification |
SG11201507273SA (en) | 2013-03-15 | 2015-10-29 | Theranos Inc | Nucleic acid amplification |
US9290800B2 (en) | 2013-03-15 | 2016-03-22 | Pacific Biosciences Of California, Inc. | Targeted rolling circle amplification |
US10294489B2 (en) | 2013-03-15 | 2019-05-21 | Board Of Trustees Of Southern Illinois University | Soybean resistant to cyst nematodes |
US9809852B2 (en) | 2013-03-15 | 2017-11-07 | Genapsys, Inc. | Systems and methods for biological analysis |
US10059999B2 (en) | 2013-06-10 | 2018-08-28 | Monsanto Technology Llc | Molecular markers associated with soybean tolerance to low iron growth conditions |
CA2922926A1 (en) | 2013-09-04 | 2015-03-12 | Fluidigm Corporation | Proximity assays for detecting nucleic acids and proteins in a single cell |
CN103421777B (zh) * | 2013-09-11 | 2015-05-13 | 北京市农林科学院林业果树研究所 | 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用 |
US10767188B2 (en) | 2013-09-25 | 2020-09-08 | Nutech Ventures | Methods and compositions for obtaining useful plant traits |
MX2016004595A (es) | 2013-10-09 | 2016-08-01 | Monsanto Technology Llc | Evento de maiz transgenico mon87403 y metodos para su deteccion. |
CN105722977A (zh) | 2013-10-30 | 2016-06-29 | 绿色生活生物技术有限责任公司 | 用于柑橘绿化枯萎病的发病定量系统和治疗方法 |
NZ718164A (en) | 2013-11-27 | 2017-04-28 | Seminis Vegetable Seeds Inc | Disease resistance loci in onion |
WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
CN106103713B (zh) | 2014-02-03 | 2021-05-28 | 赛默飞世尔科技波罗的海封闭股份公司 | 用于经控制dna片段化的方法 |
CA2939580C (en) | 2014-02-21 | 2020-07-21 | Syngenta Participations Ag | Genetic loci associated with increased fertility in maize |
NZ630710A (en) | 2014-02-27 | 2016-03-31 | Seminis Vegetable Seeds Inc | Compositions and methods for peronospora resistance in spinach |
EP3556864B1 (de) | 2014-04-18 | 2020-12-09 | Genapsys, Inc. | Verfahren und systeme zur nukleinsäureamplifikation |
GB201410022D0 (en) * | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
EP3155127B1 (de) | 2014-06-13 | 2020-07-22 | Life Technologies Corporation | Multiplex-nukleinsäureamplifikation |
US10316369B2 (en) | 2014-06-27 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and assays for male sterile watermelon |
EP3169793B1 (de) | 2014-07-16 | 2022-07-06 | Tangen Biosciences Inc. | Isothermische verfahren zur amplifizierung von nukleinsäureproben |
EP3201222B1 (de) | 2014-09-29 | 2019-04-10 | Illumina Cambridge Limited | Rekombinasemutanten |
EP3005862A1 (de) | 2014-10-10 | 2016-04-13 | Seminis Vegetable Seeds, Inc. | Melonenpflanzen mit verbesserter krankheitstoleranz |
US11559801B2 (en) | 2014-11-03 | 2023-01-24 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
EP3889274A3 (de) | 2015-04-28 | 2022-01-19 | Monsanto Technology LLC | Verfahren und zusammensetzungen zur herstellung von verkürzten maispflanzen |
UA126326C2 (uk) | 2015-04-30 | 2022-09-21 | Монсанто Текнолоджі Елелсі | Спосіб відбору рослини каноли, стійкої до кили капустяних |
ES2759477T3 (es) | 2015-05-29 | 2020-05-11 | Hoffmann La Roche | Un procedimiento de inactivación de microbios por anhídrido citracónico |
WO2016201142A1 (en) | 2015-06-09 | 2016-12-15 | Life Technologies Corporation | Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging |
US10767189B2 (en) | 2015-08-18 | 2020-09-08 | Monsanto Technology Llc | Methods for producing cotton plants with enhanced drought tolerance and compositions thereof |
US10448595B2 (en) | 2015-09-03 | 2019-10-22 | Seminis Vegetable Seeds, Inc. | Downy mildew resistant lettuce plants |
WO2017044744A2 (en) | 2015-09-10 | 2017-03-16 | Monsanto Technology Llc | Methods for producing corn plants with downy mildew resistance and compositions thereof |
GB201519565D0 (en) | 2015-11-05 | 2015-12-23 | Alere San Diego Inc | Sample preparation device |
EP3387147A1 (de) | 2015-12-09 | 2018-10-17 | Life Technologies Corporation | Nachweis und quantifizierung von nukleinsäuremolekülen mit einer oberfläche |
WO2017106731A1 (en) | 2015-12-18 | 2017-06-22 | Monsanto Technology Llc | Methods for producing corn plants with northern leaf blight resistance and compositions thereof |
US10329601B2 (en) | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
US11649484B2 (en) | 2016-02-26 | 2023-05-16 | Abbott Diagnostics Scarborough, Inc. | Redox labeled oligonucleotide probes and their use |
BR112018067739A2 (pt) | 2016-03-04 | 2019-01-08 | Alere San Diego Inc | amplificação da polimerase recombinase nested automatizada |
AU2017241670B2 (en) | 2016-03-28 | 2020-10-08 | Illumina, Inc. | Recombinase mutants |
US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
WO2017214561A1 (en) | 2016-06-10 | 2017-12-14 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
US10837067B2 (en) | 2016-08-11 | 2020-11-17 | Monsanto Technology Llc | Methods and compositions for producing corn plants with resistance to late wilt |
AU2017325833B2 (en) | 2016-09-15 | 2022-06-16 | Abbott Laboratories | Devices and methods for sample analysis |
AU2017232187B2 (en) | 2016-09-30 | 2023-11-09 | Seminis Vegetable Seeds, Inc. | Xanthomonas resistant brassica oleracea plants |
WO2018071522A1 (en) | 2016-10-11 | 2018-04-19 | Life Technologies Corporation | Rapid amplification of nucleic acids |
CN106701738B (zh) * | 2016-11-10 | 2020-05-08 | 中国科学院成都生物研究所 | 一种等温解开双链dna及制备单链dna的方法 |
JP7311420B2 (ja) | 2017-02-28 | 2023-07-19 | アボット・ダイアグノスティックス・スカボロー・インコーポレイテッド | マイクロ流体デバイスおよび関連する方法 |
US11542540B2 (en) | 2017-06-16 | 2023-01-03 | Life Technologies Corporation | Control nucleic acids, and compositions, kits, and uses thereof |
US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
RU2020100221A (ru) | 2017-08-31 | 2021-09-30 | Иониан Текнолоджис, Ллс | Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса |
SG11202002516WA (en) | 2017-09-21 | 2020-04-29 | Genapsys Inc | Systems and methods for nucleic acid sequencing |
CN111566223B (zh) | 2017-11-07 | 2024-04-12 | 生命技术公司 | 用于操纵核酸的方法和组合物 |
CN108034763B (zh) * | 2017-12-21 | 2021-10-22 | 北京卓诚惠生生物科技股份有限公司 | 检测甲型肝炎和戊型肝炎病毒的引物、探针及试剂盒 |
US20190203267A1 (en) | 2017-12-29 | 2019-07-04 | Clear Labs, Inc. | Detection of microorganisms in food samples and food processing facilities |
US10597714B2 (en) | 2017-12-29 | 2020-03-24 | Clear Labs, Inc. | Automated priming and library loading device |
GB2589159B (en) | 2017-12-29 | 2023-04-05 | Clear Labs Inc | Nucleic acid sequencing apparatus |
US11268102B2 (en) | 2018-05-16 | 2022-03-08 | University Of Florida Research Foundation, Incorporated | Compositions and methods for identifying and selecting brachytic locus in solanaceae |
EP3807420A1 (de) | 2018-06-12 | 2021-04-21 | Keygene N.V. | Verfahren zur amplifikation von nukleinsäuren |
CN108410954A (zh) * | 2018-07-10 | 2018-08-17 | 默禾医疗科技(上海)有限公司 | 一种室温扩增反应体系中反应缓冲液的制配方法 |
CA3103520A1 (en) | 2018-12-14 | 2020-06-18 | Illumina Cambridge Limited | Decreasing phasing with unlabeled nucleotides during sequencing |
KR102594019B1 (ko) | 2018-12-17 | 2023-10-26 | 일루미나 케임브리지 리미티드 | 폴리뉴클레오타이드 서열분석에 사용하기 위한 조성물 |
JP2022512265A (ja) | 2018-12-17 | 2022-02-03 | イルミナ ケンブリッジ リミテッド | シーケンシング用プライマーオリゴヌクレオチド |
CA3126761C (en) | 2019-01-15 | 2023-01-10 | Seminis Vegetable Seeds, Inc. | Green bean plants with improved disease resistance |
EP3963103B1 (de) | 2019-05-03 | 2024-02-28 | Life Technologies Corporation | Verfahren und zusammensetzungen zur manipulation von nukleinsäuren |
WO2021011850A2 (en) * | 2019-07-17 | 2021-01-21 | Chao Chien Chung | Amplification assay for the detection of anaplasma phagocytophilum |
WO2021034711A1 (en) | 2019-08-21 | 2021-02-25 | Life Technologies Corporation | System and method for sequencing |
US11542513B2 (en) | 2019-09-26 | 2023-01-03 | Seminis Vegetable Seeds, Inc. | Lettuce plants having resistance to Nasonovia ribisnigri biotype Nr:1 |
GB201916379D0 (en) | 2019-11-11 | 2019-12-25 | Biocrucible Ltd | Biochemical reaction methods and reagents |
WO2021226754A1 (zh) * | 2020-05-09 | 2021-11-18 | 杭州梓晶生物有限公司 | 一种病毒重组酶-聚合酶扩增检测方法 |
WO2021228839A1 (en) | 2020-05-11 | 2021-11-18 | Deutsches Krebsforschungszentrum | Polynucleotide detection by cas nucleases |
AU2021204717A1 (en) | 2020-07-15 | 2022-02-03 | Seminis Vegetable Seeds, Inc. | Green Bean Plants with Improved Disease Resistance |
WO2022104272A1 (en) | 2020-11-16 | 2022-05-19 | Life Technologies Corporation | System and method for sequencing |
WO2022189819A1 (en) | 2021-03-09 | 2022-09-15 | Etablissement Francais Du Sang | Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination |
CA3218077A1 (en) | 2021-04-29 | 2022-11-03 | Abbott Laboratories | Systems and methods for pooling samples for high-throughput analysis |
WO2022266946A1 (zh) * | 2021-06-24 | 2022-12-29 | 黄婉秋 | 检测病原体rna、及动物或人类基因rna的试剂盒及其应用 |
GB202110485D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
CA3226451A1 (en) | 2021-07-21 | 2023-01-26 | Sam Reed | Method and system comprising a cartridge for sequencing target polynucleotides |
GB202110479D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
WO2023014222A1 (en) | 2021-08-03 | 2023-02-09 | Wageningen Universiteit | Argonaute-based nucleic acid detection system |
US20230096386A1 (en) | 2021-09-30 | 2023-03-30 | Illumina Cambridge Limited | Polynucleotide sequencing |
CN114237548B (zh) * | 2021-11-22 | 2023-07-18 | 南京大学 | 基于非易失性存储器阵列的复数点乘运算的方法及系统 |
US20230193310A1 (en) | 2021-12-10 | 2023-06-22 | Seminis Vegetabe Seeds, Inc. | Lettuce plants having resistance to downy mildew |
US20230404003A1 (en) | 2022-06-21 | 2023-12-21 | Seminis Vegetable Seeds, Inc. | Novel qtls conferring resistance to cucumber mosaic virus |
WO2024030985A1 (en) | 2022-08-04 | 2024-02-08 | Abbott Laboratories | Assays for detecting monkeypox virus |
US20240109063A1 (en) | 2022-09-29 | 2024-04-04 | Illumina, Inc. | Dynamic optical system calibration |
Family Cites Families (91)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US603585A (en) | 1898-05-03 | cbowell | ||
US5430136A (en) | 1984-10-16 | 1995-07-04 | Chiron Corporation | Oligonucleotides having selectably cleavable and/or abasic sites |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5635347A (en) * | 1986-04-30 | 1997-06-03 | Igen, Inc. | Rapid assays for amplification products |
US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
GB8903627D0 (en) | 1989-02-17 | 1989-04-05 | Unilever Plc | Assays |
KR950013953B1 (ko) * | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
US5326692B1 (en) | 1992-05-13 | 1996-04-30 | Molecular Probes Inc | Fluorescent microparticles with controllable enhanced stokes shift |
US5273881A (en) * | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
CA2116215C (en) * | 1990-05-07 | 1998-12-22 | Tapestry Pharmaceuticals, Inc. | Diagnostic applications of double d-loop formation |
US5223414A (en) * | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
DK0540693T3 (da) | 1990-07-24 | 1999-09-13 | Hoffmann La Roche | Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5556751A (en) | 1991-04-25 | 1996-09-17 | Amoco Corporation | Selective amplification system using Q-β replicase |
EP0524349A1 (de) * | 1991-07-08 | 1993-01-27 | Opticon Sensors Europe B.V. | Abtaster mit zwei Brennweiten und die Verwendung eines solchen Abtasters als Lesestift für Kodierungen |
EP0543484B1 (de) * | 1991-08-30 | 2001-01-31 | Research Development Corporation of Japan | Verfahren zur Amplifikation von DNA |
WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
CA2122203C (en) | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
FR2708288B1 (fr) * | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
US5942391A (en) | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
US5705366A (en) | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
US5656430A (en) | 1995-06-07 | 1997-08-12 | Trevigen, Inc. | Oscillating signal amplifier for nucleic acid detection |
US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5731150A (en) | 1995-11-01 | 1998-03-24 | Chiron Diagnostic Corporation | IS6110 based molecular detection of mycobacterium tuberculosis |
US5985561A (en) * | 1996-06-03 | 1999-11-16 | New York Society For The Relief Of The Ruptured And Crippled Maintaining The Hospital For Special Surgery | FC receptor polymorphism |
US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
CA2263815A1 (en) * | 1996-08-29 | 1998-03-05 | Koji Kigawa | Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase |
WO1998045474A1 (en) | 1997-04-04 | 1998-10-15 | Innogenetics N.V. | Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions |
US6245506B1 (en) | 1997-07-30 | 2001-06-12 | Bbi Bioseq, Inc. | Integrated sequencing device |
AU753661B2 (en) | 1998-04-22 | 2002-10-24 | Enterprise Ireland Trading As Bioresearch Ireland | A method for the characterisation of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site |
WO1999060158A1 (fr) | 1998-05-19 | 1999-11-25 | Laboratory Of Molecular Biophotonics | Phase solide utilisee pour detecter de l'acide nucleique et procede de detection d'acide nucleique |
US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
US6087112A (en) * | 1998-12-30 | 2000-07-11 | Oligos Etc. Inc. | Arrays with modified oligonucleotide and polynucleotide compositions |
JP2003526328A (ja) | 1999-01-11 | 2003-09-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | Dnaの等温増幅 |
WO2000046408A1 (en) | 1999-02-04 | 2000-08-10 | Sloan-Kettering Institute For Cancer Research | Process for dna replication |
US6699693B1 (en) * | 1999-02-04 | 2004-03-02 | Sloan-Kettering Institute For Cancer Research | Process for DNA replication |
US6387621B1 (en) | 1999-04-27 | 2002-05-14 | University Of Utah Research Foundation | Automated analysis of real-time nucleic acid amplification |
US6509157B1 (en) * | 1999-11-05 | 2003-01-21 | Roche Molecular Systems, Inc | 3 blocked nucleic acid amplification primers |
NO314091B1 (no) * | 2000-01-12 | 2003-01-27 | Biotec Pharmacon Asa | Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet |
US20010044111A1 (en) | 2000-03-20 | 2001-11-22 | Brian Carr | Method for generating recombinant DNA molecules in complex mixtures |
PT1278889E (pt) | 2000-03-29 | 2006-12-29 | Lgc Ltd | Farol de hibridização e método para a detecção e discriminação rápida de sequência |
US20020061530A1 (en) | 2000-07-31 | 2002-05-23 | Belotserkovskii Boris P. | Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation |
IE20000887A1 (en) | 2000-11-03 | 2002-12-11 | Univ College Cork Nat Univ Ie | Method for the amplification and optional characterisation of nucleic acids |
US6379899B1 (en) * | 2001-03-13 | 2002-04-30 | Discoverx | Isothermal exponential RNA amplification in complex mixtures |
ATE397095T1 (de) | 2001-04-20 | 2008-06-15 | Penn State Res Found | Verfahren zur manipulation von nukleinsäuren |
EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
US7112423B2 (en) | 2001-07-15 | 2006-09-26 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
CA2492423A1 (en) | 2001-07-15 | 2004-03-18 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
CA2458127A1 (en) | 2001-08-20 | 2003-02-27 | Takara Bio Inc. | Nucleic acid amplification methods |
AU2002341898A2 (en) | 2001-09-28 | 2003-04-07 | University Of Delaware | Polymorphism detection and separation |
KR100445560B1 (ko) | 2001-10-31 | 2004-08-21 | (주)바이오넥스 | 핵산 또는 생물학적 물질을 분리하기 위한 키트의 제조방법과, 그 방법에 의해 제조된 키트와, 그 키트를사용하는 장치 |
US7244562B2 (en) * | 2001-11-01 | 2007-07-17 | Gene Check, Inc. | RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences |
US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
US20040137456A1 (en) * | 2002-04-04 | 2004-07-15 | Hiroki Yokota | Method for identifying and characterizing individual dna molecules |
US20030228611A1 (en) | 2002-05-01 | 2003-12-11 | President And Fellows Of Harvard College | Nucleic acid memory device |
US6713262B2 (en) | 2002-06-25 | 2004-03-30 | Agilent Technologies, Inc. | Methods and compositions for high throughput identification of protein/nucleic acid binding pairs |
AU2003260679A1 (en) | 2002-07-12 | 2004-02-02 | British Biocell International Limited | Lateral flow assay device and method |
US20040038213A1 (en) | 2002-08-06 | 2004-02-26 | Kwon Jai W. | Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy |
EP1543154A4 (de) | 2002-08-21 | 2006-08-16 | Epoch Biosciences Inc | Assay mit endonuklease für abasische stellen |
CA2498764C (en) * | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
US7662594B2 (en) | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
EP1613768A4 (de) * | 2003-03-11 | 2006-05-31 | Gene Check Inc | Reca-unterstütztes allelspezifisches oligonukleotidextensionsverfahren |
DE10315640A1 (de) | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
EP1564306B1 (de) | 2004-02-17 | 2013-08-07 | Affymetrix, Inc. | Verfahren zur Fragmentierung und Markierung von DNS |
JP5026958B2 (ja) | 2004-06-01 | 2012-09-19 | アリーア サン ディエゴ, インコーポレイテッド | リコンビナーゼポリメラーゼ増幅 |
US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
ATE396282T1 (de) | 2004-10-11 | 2008-06-15 | Epigenomics Ag | Verfahren zum kontaminationsschutz von dna- amplifikationsreaktionen zur methylierungsanalyse durch modifizierte vorbehandlung der nukleinsäuren |
US20060110765A1 (en) | 2004-11-23 | 2006-05-25 | Wang Xiao B | Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method |
US20070042427A1 (en) | 2005-05-03 | 2007-02-22 | Micronics, Inc. | Microfluidic laminar flow detection strip |
WO2006119280A2 (en) | 2005-05-03 | 2006-11-09 | Handylab, Inc. | Lyophilized pellets |
CN101203618B (zh) | 2005-05-26 | 2013-03-13 | 人类遗传标记控股有限公司 | 使用含有非常规碱基的引物的等温链置换扩增 |
WO2007008997A2 (en) | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
EP1929049B1 (de) * | 2005-07-25 | 2013-04-10 | Alere San Diego, Inc. | Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung |
AU2007298650B2 (en) * | 2006-05-04 | 2013-10-17 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
CN101855362A (zh) | 2007-05-18 | 2010-10-06 | 美国政府健康及人类服务部,疾病控制和预防中心 | 用于检测肺炎链球菌的引物和探针 |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
ES2320840B1 (es) | 2007-11-27 | 2010-07-01 | Nelson Alfonso Egued | Tubo humectador de doble camara. |
JP2009139487A (ja) | 2007-12-04 | 2009-06-25 | Nippon Sheet Glass Co Ltd | 正立等倍レンズアレイプレート |
JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質 |
WO2010141940A1 (en) | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
US10927393B2 (en) | 2012-06-08 | 2021-02-23 | Ionian Technologies, Llc | Nucleic acid amplifications |
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