SG11201809242VA - Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor - Google Patents

Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor

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Publication number
SG11201809242VA
SG11201809242VA SG11201809242VA SG11201809242VA SG11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA
Authority
SG
Singapore
Prior art keywords
used therefor
molecular complex
genome sequence
introduction efficiency
modification technique
Prior art date
Application number
SG11201809242VA
Inventor
Keiji Nishida
Akihiko Kondo
Takayuki Arazoe
Zenpei Shimatani
Original Assignee
Univ Kobe Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Publication of SG11201809242VA publication Critical patent/SG11201809242VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

Abstract

METHOD FOR INCREASING MUTATION INTRODUCTION EFFICIENCY IN GENOME SEQUENCE MODIFICATION TECHNIQUE, AND MOLECULAR COMPLEX TO BE USED THEREFOR The present invention provides a method of modifying a 5 targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence- recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and 10 culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site. FIG. 2 15
SG11201809242VA 2016-04-21 2017-04-21 Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor SG11201809242VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016085631 2016-04-21
PCT/JP2017/016105 WO2017183724A1 (en) 2016-04-21 2017-04-21 Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor

Publications (1)

Publication Number Publication Date
SG11201809242VA true SG11201809242VA (en) 2018-11-29

Family

ID=60116870

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201809242VA SG11201809242VA (en) 2016-04-21 2017-04-21 Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor

Country Status (11)

Country Link
US (1) US20200377910A1 (en)
EP (1) EP3447139B1 (en)
JP (1) JP7001272B2 (en)
KR (1) KR102116200B1 (en)
CN (1) CN109312329B (en)
BR (1) BR112018071376A2 (en)
CA (1) CA3021281C (en)
DK (1) DK3447139T3 (en)
ES (1) ES2919961T3 (en)
SG (1) SG11201809242VA (en)
WO (1) WO2017183724A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108495932B (en) 2015-11-27 2022-08-09 国立大学法人神户大学 Method for converting genomic sequence of monocotyledon for specifically converting nucleobase targeting DNA sequence, and molecular complex used therefor
KR20200103769A (en) * 2018-01-23 2020-09-02 기초과학연구원 Extended single guide RNA and uses thereof
CN110607320B (en) * 2018-11-23 2023-05-12 电子科技大学 Plant genome directional base editing framework vector and application thereof
WO2020241869A1 (en) * 2019-05-30 2020-12-03 国立大学法人東京大学 GENOME EDITING SYSTEM USING Cas PROTEIN HAVING TWO TYPES OF NUCLEIC ACID BASE-CONVERTING ENZYMES FUSED THERETO
WO2021046155A1 (en) 2019-09-03 2021-03-11 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
JP2013128413A (en) 2010-03-11 2013-07-04 Kyushu Univ Method for modifying rna-binding protein using ppr motif
DK3115457T3 (en) * 2014-03-05 2019-11-04 Univ Kobe Nat Univ Corp PROCEDURE FOR MODIFYING GENE SEQUENCE TO SPECIFICALLY CONVERT THE NUCLEIC ACID BASES OF TARGETED DNA SEQUENCE AND MOLECULAR COMPLEX TO USE IN SAME
EP3216867B1 (en) * 2014-11-04 2020-04-15 National University Corporation Kobe University Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein
WO2017009073A1 (en) * 2015-07-13 2017-01-19 Huf Hülsbeck & Fürst Gmbh & Co. Kg Exterior door handle for a vehicle
US20190024098A1 (en) * 2015-09-09 2019-01-24 National University Corporation Kobe University Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method
WO2017043656A1 (en) * 2015-09-09 2017-03-16 国立大学法人神戸大学 Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted dna sequence, and molecular complex used in same
CA3002827A1 (en) * 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
CN108495932B (en) * 2015-11-27 2022-08-09 国立大学法人神户大学 Method for converting genomic sequence of monocotyledon for specifically converting nucleobase targeting DNA sequence, and molecular complex used therefor

Also Published As

Publication number Publication date
CN109312329B (en) 2023-11-21
ES2919961T3 (en) 2022-07-29
DK3447139T3 (en) 2022-07-04
CA3021281A1 (en) 2017-10-26
EP3447139B1 (en) 2022-06-08
KR20180132862A (en) 2018-12-12
WO2017183724A1 (en) 2017-10-26
EP3447139A4 (en) 2019-10-02
JPWO2017183724A1 (en) 2019-02-28
CA3021281C (en) 2021-07-27
CN109312329A (en) 2019-02-05
JP7001272B2 (en) 2022-01-19
US20200377910A1 (en) 2020-12-03
KR102116200B1 (en) 2020-05-27
EP3447139A1 (en) 2019-02-27
BR112018071376A2 (en) 2019-04-24

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