MY184632A - A method of identifying parentage in freshwater prawn macrobrachium rosenbergii - Google Patents

A method of identifying parentage in freshwater prawn macrobrachium rosenbergii

Info

Publication number
MY184632A
MY184632A MYPI2013701608A MYPI2013701608A MY184632A MY 184632 A MY184632 A MY 184632A MY PI2013701608 A MYPI2013701608 A MY PI2013701608A MY PI2013701608 A MYPI2013701608 A MY PI2013701608A MY 184632 A MY184632 A MY 184632A
Authority
MY
Malaysia
Prior art keywords
seq
macrobrachium rosenbergii
parentage
identifying
amplicons
Prior art date
Application number
MYPI2013701608A
Inventor
Bhassu Subha
Easwvaran Sarasvathi
Yasmin Othman Rofina
Original Assignee
Univ Malaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Malaya filed Critical Univ Malaya
Priority to MYPI2013701608A priority Critical patent/MY184632A/en
Priority to PCT/MY2014/000229 priority patent/WO2015034347A1/en
Publication of MY184632A publication Critical patent/MY184632A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like

Abstract

A method of identifying parentage in Macrobrachium rosenbergii prawn comprising the steps of amplifying targeted EST-SSR nucleic acid sequences from a biological sample of Macrobrachium rosenbergii through a polymerase chain reaction using primer pairs with nucleic acid sequences as set forth in SEQ ID NO. 1 and SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6, SEQ ID NO. 7 and SEQ ID NO. 8, SEQ ID NO. 9 and SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12 to produce respective amplicons; determining fragment size of the produced amplicons; regarding the fragment size of the amplicons corresponding to predetermined alleles; and identifying possible parentage of the prawn based on the regarded allele.
MYPI2013701608A 2013-09-09 2013-09-09 A method of identifying parentage in freshwater prawn macrobrachium rosenbergii MY184632A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2013701608A MY184632A (en) 2013-09-09 2013-09-09 A method of identifying parentage in freshwater prawn macrobrachium rosenbergii
PCT/MY2014/000229 WO2015034347A1 (en) 2013-09-09 2014-09-09 A method of identifying parentage in freshwater prawn macrobrachium rosenbergii

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2013701608A MY184632A (en) 2013-09-09 2013-09-09 A method of identifying parentage in freshwater prawn macrobrachium rosenbergii

Publications (1)

Publication Number Publication Date
MY184632A true MY184632A (en) 2021-04-12

Family

ID=52628707

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2013701608A MY184632A (en) 2013-09-09 2013-09-09 A method of identifying parentage in freshwater prawn macrobrachium rosenbergii

Country Status (2)

Country Link
MY (1) MY184632A (en)
WO (1) WO2015034347A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105349651B (en) * 2015-11-18 2018-05-04 广东省中药研究所 The method and primer of EST-SSR Marker Identification Chinese medicine rabdosia lophanthide kinds
CN109797226A (en) * 2019-02-26 2019-05-24 中国水产科学研究院珠江水产研究所 A kind of Macrobrachium rosenbergii classification method based on EST-SSR label
CN110016510B (en) * 2019-04-22 2022-06-07 宁波大学 Molecular marker for genetic breeding of macrobrachium rosenbergii
CN111996261B (en) * 2020-07-31 2023-04-28 湖南银鱼农业科技有限公司 Macrobrachium rosenbergii sex molecular marker primer and application thereof
CN113881782A (en) * 2021-10-25 2022-01-04 上海海洋大学 Primer group, kit, identification method and application for identifying sex of macrobrachium rosenbergii
CN114395635B (en) * 2022-03-11 2023-07-28 广西壮族自治区水产科学研究院 SNP molecular marker related to growth traits of macrobrachium rosenbergii and application of SNP molecular marker
CN114561479B (en) * 2022-03-25 2023-03-17 浙江省淡水水产研究所 Primer for identifying iron-shell shrimp individuals in macrobrachium rosenbergii and application of primer

Also Published As

Publication number Publication date
WO2015034347A1 (en) 2015-03-12

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