MY174088A - A method of detecting porcine markers for identifying pork content in a sample - Google Patents
A method of detecting porcine markers for identifying pork content in a sampleInfo
- Publication number
- MY174088A MY174088A MYPI2014702291A MYPI2014702291A MY174088A MY 174088 A MY174088 A MY 174088A MY PI2014702291 A MYPI2014702291 A MY PI2014702291A MY PI2014702291 A MYPI2014702291 A MY PI2014702291A MY 174088 A MY174088 A MY 174088A
- Authority
- MY
- Malaysia
- Prior art keywords
- sample
- markers
- comprises seq
- porcine
- reverse primer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
Abstract
The present invention relates to a method of detecting porcine markers for identifying a pork content in a test sample, comprising the steps of: extracting deoxyribonucleic acid (DNA) from the sample; performing polymerase chain reaction (PCR) on the extracted DNA using two pairs of primers, including a first forward primer and a first reverse primer for amplifying a target porcine DNA sequence, and a second forward primer and a second reverse primer (as an internal control for amplifying a target gene sequence; then subjecting the amplified sequence to electrophoresis to detect the presence of porcine markers in the sample; characterized in that: the first forward primer comprises SEQ ID N0:1, the first reverse primer comprises SEQ ID N0:2, the second forward primer comprises SEQ ID N0:3, and the second reverse primer comprises SEQ ID N0:4.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2014702291A MY174088A (en) | 2014-08-18 | 2014-08-18 | A method of detecting porcine markers for identifying pork content in a sample |
PCT/MY2015/050016 WO2016028138A1 (en) | 2014-08-18 | 2015-03-19 | A method of detecting porcine markers for identifying pork content in a sample |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2014702291A MY174088A (en) | 2014-08-18 | 2014-08-18 | A method of detecting porcine markers for identifying pork content in a sample |
Publications (1)
Publication Number | Publication Date |
---|---|
MY174088A true MY174088A (en) | 2020-03-09 |
Family
ID=55350999
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MYPI2014702291A MY174088A (en) | 2014-08-18 | 2014-08-18 | A method of detecting porcine markers for identifying pork content in a sample |
Country Status (2)
Country | Link |
---|---|
MY (1) | MY174088A (en) |
WO (1) | WO2016028138A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108118086A (en) * | 2018-01-30 | 2018-06-05 | 西北民族大学 | For detecting RPA primers, probe and the method for pork content in meat products |
CN111440881B (en) * | 2020-05-27 | 2023-11-03 | 兰州海关技术中心 | Primer group, kit and detection method for detecting pork and application |
-
2014
- 2014-08-18 MY MYPI2014702291A patent/MY174088A/en unknown
-
2015
- 2015-03-19 WO PCT/MY2015/050016 patent/WO2016028138A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2016028138A1 (en) | 2016-02-25 |
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