TW201613950A - Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof - Google Patents

Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof

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Publication number
TW201613950A
TW201613950A TW103134818A TW103134818A TW201613950A TW 201613950 A TW201613950 A TW 201613950A TW 103134818 A TW103134818 A TW 103134818A TW 103134818 A TW103134818 A TW 103134818A TW 201613950 A TW201613950 A TW 201613950A
Authority
TW
Taiwan
Prior art keywords
oligo
identification
nucleotide probe
specific primers
feline calicivirus
Prior art date
Application number
TW103134818A
Other languages
Chinese (zh)
Other versions
TWI515200B (en
Inventor
Hsiao-Fen Chang
Pei-Yu Lee
Yun-Lung Tsai
fu-chun Li
Jian-Hao Ciou
Original Assignee
Genereach Biotechnology Corp
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Publication date
Application filed by Genereach Biotechnology Corp filed Critical Genereach Biotechnology Corp
Priority to TW103134818A priority Critical patent/TWI515200B/en
Application granted granted Critical
Publication of TWI515200B publication Critical patent/TWI515200B/en
Publication of TW201613950A publication Critical patent/TW201613950A/en

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Abstract

The present invention provides specific primers, an oligo-nucleotide probe for feline calicivirus identification, and an identification method thereof. The identification method includes: (a) extracting nucleic acid from a sample; (b) mixing the nucleic acid with the specific primers, the oligo-nucleotide probe, dNTPs, and Taq DNA polymerase into a mixture in a reaction tube; (c) placing the reaction tube into the iiPCR system to carry out reverse transcription and iiPCR reaction, so as to amplify the target gene and detect a fluorescence signal emitted by reporter dye of the oligo-nucleotide probe; and (d) identifying the result of fluorescence signal to determine whether the sample contains feline calicivirus or not.
TW103134818A 2014-10-06 2014-10-06 Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof TWI515200B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW103134818A TWI515200B (en) 2014-10-06 2014-10-06 Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW103134818A TWI515200B (en) 2014-10-06 2014-10-06 Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof

Publications (2)

Publication Number Publication Date
TWI515200B TWI515200B (en) 2016-01-01
TW201613950A true TW201613950A (en) 2016-04-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
TW103134818A TWI515200B (en) 2014-10-06 2014-10-06 Specific primers, oligo-nucleotide probe for feline calicivirus identification, and identification method thereof

Country Status (1)

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TW (1) TWI515200B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107099608A (en) * 2017-06-13 2017-08-29 中国农业科学院兰州兽医研究所 A kind of iiPCR kits for detecting mycoplasma capri goat pneumonia subspecies

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107099608A (en) * 2017-06-13 2017-08-29 中国农业科学院兰州兽医研究所 A kind of iiPCR kits for detecting mycoplasma capri goat pneumonia subspecies

Also Published As

Publication number Publication date
TWI515200B (en) 2016-01-01

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