SG11201811799VA - Primer set, kit and method for detecting two or more target nucleic acids - Google Patents

Primer set, kit and method for detecting two or more target nucleic acids

Info

Publication number
SG11201811799VA
SG11201811799VA SG11201811799VA SG11201811799VA SG11201811799VA SG 11201811799V A SG11201811799V A SG 11201811799VA SG 11201811799V A SG11201811799V A SG 11201811799VA SG 11201811799V A SG11201811799V A SG 11201811799VA SG 11201811799V A SG11201811799V A SG 11201811799VA
Authority
SG
Singapore
Prior art keywords
target nucleic
terminal part
nucleotide sequence
detecting
nucleic acids
Prior art date
Application number
SG11201811799VA
Inventor
Shigehiko Miyamoto
Takashi Nishizono
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Publication of SG11201811799VA publication Critical patent/SG11201811799VA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

PRIMER SET, KIT AND METHOD FOR DETECTING TWO OR MORE TARGET NUCLEIC ACIDS The present invention provides an improved approach for detecting two or more target nucleic acids. The primer set of the present invention comprises: terminal primer A comprising a polynucleotide comprising, in its 3'-terminal part, a nucleotide sequence that hybridizes to a 3'-terminal part of a complementary sequence of a first target nucleic acid sequence; a kth double-headed primer comprising two polynucleotides linked at their 5' terminal sides, wherein one of the two polynucleotides comprises, in its 3'-terminal part, a nucleotide sequence that hybridizes to a 3'-terminal part of the nucleotide sequence of a kth target nucleic acid, and the other of the two polynucleotides comprises, in its 3'-terminal part, a nucleotide sequence that hybridizes to a 3'-terminal part of a complementary sequence of the nucleotide sequence of a (k + 1)th target nucleic acid; and terminal primer B comprising a polynucleotide comprising, in its 3'-terminal part, a nucleotide sequence that hybridizes to a 3'- terminal part of the nucleotide sequence of a Nth target nucleic acid. [No Suitable Figure]
SG11201811799VA 2016-07-01 2017-06-16 Primer set, kit and method for detecting two or more target nucleic acids SG11201811799VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016132066 2016-07-01
PCT/JP2017/022314 WO2018003550A1 (en) 2016-07-01 2017-06-16 Primer set, kit and method for detecting two or more target nucleic acids

Publications (1)

Publication Number Publication Date
SG11201811799VA true SG11201811799VA (en) 2019-02-27

Family

ID=60786011

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201811799VA SG11201811799VA (en) 2016-07-01 2017-06-16 Primer set, kit and method for detecting two or more target nucleic acids

Country Status (6)

Country Link
US (1) US11274339B2 (en)
EP (1) EP3480309A4 (en)
JP (1) JP7030051B2 (en)
CN (1) CN109415721B (en)
SG (1) SG11201811799VA (en)
WO (1) WO2018003550A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102105558B1 (en) * 2018-03-23 2020-04-28 (주)바이오니아 Analysis Plate For Polymerase Chain Reaction
CN114901817A (en) * 2019-12-27 2022-08-12 株式会社钟化 Primer set and method for detecting target nucleic acid using same
CN116574780B (en) * 2023-02-24 2023-11-10 苏州唯善生物科技有限公司 Design method of primer probe for simultaneously detecting two miRNAs through single fluorescent channel, kit and quantitative detection method

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6054274A (en) * 1997-11-12 2000-04-25 Hewlett-Packard Company Method of amplifying the signal of target nucleic acid sequence analyte
US8460866B2 (en) * 2006-03-01 2013-06-11 Keygene N.V. High throughput sequence-based detection of SNPs using ligation assays
JP5435687B2 (en) * 2008-01-11 2014-03-05 国立大学法人徳島大学 Double-label fusion PCR immunochromatography
JP2009268360A (en) * 2008-04-30 2009-11-19 Yamaguchi Univ Primer for producing fused dna fragment and method for producing fused dna fragment using the same
EP2644699B1 (en) 2010-11-24 2018-02-21 Kaneka Corporation Amplified nucleic acid detection method and detection device
JP5279051B2 (en) 2011-08-10 2013-09-04 独立行政法人農業・食品産業技術総合研究機構 Oligonucleotides for detecting Salmonella Typhimurium and rapid serotype identification method using them
AU2013293240A1 (en) * 2012-07-24 2015-03-05 Adaptive Biotechnologies Corp. Single cell analysis using sequence tags
JP6547302B2 (en) 2015-01-19 2019-07-24 いすゞ自動車株式会社 Coolant debris recovery system

Also Published As

Publication number Publication date
WO2018003550A1 (en) 2018-01-04
US20190119727A1 (en) 2019-04-25
CN109415721B (en) 2022-04-22
EP3480309A1 (en) 2019-05-08
US11274339B2 (en) 2022-03-15
EP3480309A4 (en) 2020-07-29
JP7030051B2 (en) 2022-03-04
JPWO2018003550A1 (en) 2019-04-18
CN109415721A (en) 2019-03-01

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