IN2014DN07968A - - Google Patents

Info

Publication number
IN2014DN07968A
IN2014DN07968A IN7968DEN2014A IN2014DN07968A IN 2014DN07968 A IN2014DN07968 A IN 2014DN07968A IN 7968DEN2014 A IN7968DEN2014 A IN 7968DEN2014A IN 2014DN07968 A IN2014DN07968 A IN 2014DN07968A
Authority
IN
India
Prior art keywords
seq
biological sample
virus
set forth
primer pair
Prior art date
Application number
Inventor
Subha A P Bhassu
Othman Rofina Yasmin Binti
Kua Beng Chu
Too Lay Khoon
Original Assignee
Univ Malaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Malaya filed Critical Univ Malaya
Publication of IN2014DN07968A publication Critical patent/IN2014DN07968A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for quantitatively detecting infectious hypodermal and haematopoietic necrosis virus (IHHNV) in a biological sample, comprising the steps of obtaining a primer pair having nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, and a fluorescent probe having a nucleotide sequence set forth in SEQ ID NO: 3; amplifying a non-structural protein coding region of the virus in the biological sample by a real-time polymerase chain reaction (PCR) using the primer pair and the fluorescent probe; and determining the presence and quantity of the virus in the biological sample with reference to a positive control plasmid.
IN7968DEN2014 2012-02-27 2013-01-15 IN2014DN07968A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2012700037A MY184600A (en) 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus
PCT/MY2013/000004 WO2013129906A1 (en) 2012-02-27 2013-01-15 Real-time viral detection method and kit for infectious hypodermaland haematopoietic necrosis virus

Publications (1)

Publication Number Publication Date
IN2014DN07968A true IN2014DN07968A (en) 2015-05-01

Family

ID=49083015

Family Applications (1)

Application Number Title Priority Date Filing Date
IN7968DEN2014 IN2014DN07968A (en) 2012-02-27 2013-01-15

Country Status (4)

Country Link
CN (1) CN104204231A (en)
IN (1) IN2014DN07968A (en)
MY (1) MY184600A (en)
WO (1) WO2013129906A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108531657A (en) * 2018-05-09 2018-09-14 鲁东大学 The fluorescence quantitative PCR detection primer sets and detection kit of prawn infectious subcutaneous and haematopoietic necrosis virus
CN112176111A (en) * 2020-11-05 2021-01-05 北京市水产技术推广站 Primer, probe, kit and detection method for detecting infectious pancreatic necrosis virus
CN116200539A (en) * 2022-12-19 2023-06-02 中国海洋大学三亚海洋研究院 ERA technology-based method for rapidly detecting infectious subcutaneous and hematopoietic necrosis viruses

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410549A (en) * 2001-09-21 2003-04-16 中国科学院南海海洋研究所 Fast detection prawn infectious subcutaneous and hemopoiesis tissue necrosis virus reagent box
CN100351630C (en) * 2005-10-23 2007-11-28 王学海 Kit for diagnosing FQ-PCR of prawn IHHNV and its testing method
BRPI0714538A2 (en) * 2006-08-24 2013-11-26 Du Pont IHHNV DIAGNOSTIC PRIMER SEQUENCES ISOLATED, PAIR OF DIFFERENT IHHNV DIAGNOSTIC PRIMER SEQUENCES, IHHNV DETECTION KIT, IHHNV PRESENCE DETECTION METHODS AND WHICH METHOD OF IHHNV PRESENCE
CN100460519C (en) * 2006-08-29 2009-02-11 中国科学院南海海洋研究所 Reagent box for detecting prawn infectious subcutaneous and hemopoietic tissue necrosis virus and method thereof
CN101643793A (en) * 2009-08-27 2010-02-10 中国科学院南海海洋研究所 Reagent kit for testing infectious hypodermic and hematopoietic necrosis viruses of prawns, and testing method thereof
CN101792817B (en) * 2009-11-30 2012-07-25 浙江大学 Detection method of prawn IHHNV (infectious hypodermal and hematopoietic necrosis virus) and used nucleic acid isothermal amplification detection kit

Also Published As

Publication number Publication date
MY184600A (en) 2021-04-07
CN104204231A (en) 2014-12-10
WO2013129906A1 (en) 2013-09-06

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