SG11202002516WA - Systems and methods for nucleic acid sequencing - Google Patents

Systems and methods for nucleic acid sequencing

Info

Publication number
SG11202002516WA
SG11202002516WA SG11202002516WA SG11202002516WA SG11202002516WA SG 11202002516W A SG11202002516W A SG 11202002516WA SG 11202002516W A SG11202002516W A SG 11202002516WA SG 11202002516W A SG11202002516W A SG 11202002516WA SG 11202002516W A SG11202002516W A SG 11202002516WA
Authority
SG
Singapore
Prior art keywords
systems
methods
nucleic acid
acid sequencing
sequencing
Prior art date
Application number
SG11202002516WA
Inventor
Hesaam Esfandyarpour
Maryam Jouzi
Seth Stern
Paul Kenney
Original Assignee
Genapsys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genapsys Inc filed Critical Genapsys Inc
Publication of SG11202002516WA publication Critical patent/SG11202002516WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/30—Detection characterised by liberation or release of label
SG11202002516WA 2017-09-21 2018-09-20 Systems and methods for nucleic acid sequencing SG11202002516WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762561358P 2017-09-21 2017-09-21
US201862655083P 2018-04-09 2018-04-09
PCT/US2018/052072 WO2019060628A1 (en) 2017-09-21 2018-09-20 Systems and methods for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
SG11202002516WA true SG11202002516WA (en) 2020-04-29

Family

ID=65810962

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202002516WA SG11202002516WA (en) 2017-09-21 2018-09-20 Systems and methods for nucleic acid sequencing

Country Status (9)

Country Link
US (2) US10900075B2 (en)
EP (1) EP3684951A4 (en)
JP (2) JP2021500858A (en)
CN (1) CN111566224A (en)
CA (1) CA3076378A1 (en)
IL (1) IL273426A (en)
MX (1) MX2020003113A (en)
SG (1) SG11202002516WA (en)
WO (1) WO2019060628A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012047889A2 (en) 2010-10-04 2012-04-12 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
MX2020003113A (en) 2017-09-21 2020-09-07 Genapsys Inc Systems and methods for nucleic acid sequencing.
CN113905822B (en) * 2019-04-12 2023-09-22 西部数据技术公司 Nucleic acid sequencing with synthesis using a magnetic sensor array
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags

Family Cites Families (239)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1049467A (en) 1907-05-29 1913-01-07 Chadeloid Chemical Co Paint or varnish remover.
US1053321A (en) 1909-05-20 1913-02-18 Otto E Schrock Rotary pump and motor.
US1026689A (en) 1909-08-30 1912-05-21 Peter A Mccullough Trolley-harp.
US1009397A (en) 1911-01-21 1911-11-21 Henry W Falstrom Buckle.
US1012539A (en) 1911-02-09 1911-12-19 Geiser Mfg Company Set-works for sawmills.
US1010035A (en) 1911-02-23 1911-11-28 American Steel Foundries Reinforced car-truck.
US1047267A (en) 1911-06-21 1912-12-17 Jeffrey Mfg Co Anchor.
US1005998A (en) 1911-07-10 1911-10-17 Arthur Munchausen Berry-carrier.
US1026009A (en) 1911-09-23 1912-05-14 Tetsusaburo Watanabe Fountain paint-brush.
US1053952A (en) 1911-11-25 1913-02-18 Basf Ag Process of manufacturing ammonia.
US1061209A (en) 1912-04-22 1913-05-06 William Monroe White Life-saving device.
US1054445A (en) 1912-11-16 1913-02-25 Jan Orlowski Umbrella.
US1057044A (en) 1913-01-16 1913-03-25 Benjamin Douglass Jr Pneumatic antislip device for power-driven vehicles.
US2014761A (en) 1929-12-05 1935-09-17 Waldhof Zellstoff Fab Process for the manufacture of solutions from cellulose
SE407115B (en) 1975-10-06 1979-03-12 Kabi Ab PROCEDURES AND METAL ELECTRODES FOR THE STUDY OF ENZYMATIC AND OTHER BIOCHEMICAL REACTIONS
US4959615A (en) 1988-05-31 1990-09-25 Micro Encoder, Inc. Electrode structure for capacitance-type measurement transducers
US5612181A (en) 1989-06-21 1997-03-18 Fourmentin-Guilbert; Jean E. R. Method for arranging a polynucleotide on a substrate for point-by-point analysis of the bases thereof
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
JP3025027B2 (en) 1991-01-21 2000-03-27 株式会社日立製作所 Oxygen sensor
RU1794088C (en) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Method of dna nucleotide sequence determination and a device for its realization
WO1993008464A1 (en) 1991-10-21 1993-04-29 Holm Kennedy James W Method and device for biochemical sensing
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5602042A (en) 1994-04-14 1997-02-11 Cytyc Corporation Method and apparatus for magnetically separating biological particles from a mixture
DE69515675T2 (en) 1994-05-11 2000-07-20 Genera Technologies Ltd Process for capturing a ligand from a liquid and device for carrying it out
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
ES2563643T3 (en) 1997-04-01 2016-03-15 Illumina Cambridge Limited Nucleic acid sequencing method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
ATE556149T1 (en) 1999-02-23 2012-05-15 Caliper Life Sciences Inc MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
CN1181337C (en) 2000-08-08 2004-12-22 清华大学 Solid molecule operating method in microfluid system
US6046097A (en) 1999-03-23 2000-04-04 United Microelectronics Corp. Deposition method with improved step coverage
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
EP1208240A4 (en) 1999-08-26 2006-10-04 Univ Princeton Microfluidic and nanofluidic electronic devices for detecting changes in capacitance of fluids and methods of using
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
WO2001037958A2 (en) 1999-11-04 2001-05-31 Princeton University Electrodeless dielectrophoresis for polarizable particles
US6616819B1 (en) 1999-11-04 2003-09-09 Therasense, Inc. Small volume in vitro analyte sensor and methods
WO2001037291A1 (en) 1999-11-17 2001-05-25 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
WO2001042508A2 (en) 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US7682837B2 (en) 2000-05-05 2010-03-23 Board Of Trustees Of Leland Stanford Junior University Devices and methods to form a randomly ordered array of magnetic beads and uses thereof
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
CA2421939A1 (en) 2000-09-27 2002-04-04 Aviva Biosciences Corporation Apparatus for switchng and manipulating particles and method of use thereof
EP3034627B1 (en) * 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
AU2002241803A1 (en) 2000-10-20 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Transient electrical signal based methods and devices for characterizing molecular interaction and/or motion in a sample
CN1483078A (en) 2000-10-27 2004-03-17 荣研化学株式会社 Method of synthesizing single-stranded nucleic acid
US20030209432A1 (en) 2000-12-11 2003-11-13 Choong Vi-En Methods and compositions relating to electrical detection of nucleic acid reactions
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002061146A1 (en) 2001-02-01 2002-08-08 The Trustees Of Columbia University In The City Of New York Use of electrical fields to control interactions between proteins and nucleic acid constructions immobilized on solid supports
GB0105831D0 (en) 2001-03-09 2001-04-25 Toumaz Technology Ltd Method for dna sequencing utilising enzyme linked field effect transistors
US8114591B2 (en) 2001-03-09 2012-02-14 Dna Electronics Ltd. Sensing apparatus and method
US20040023253A1 (en) 2001-06-11 2004-02-05 Sandeep Kunwar Device structure for closely spaced electrodes
US20030096268A1 (en) 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
ATE400663T1 (en) 2002-02-21 2008-07-15 Asm Scient Inc RECOMBINASE-POLYMERASE AMPLIFICATION
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US6953958B2 (en) 2002-03-19 2005-10-11 Cornell Research Foundation, Inc. Electronic gain cell based charge sensor
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
DE10221799A1 (en) 2002-05-15 2003-11-27 Fujitsu Ltd Semiconductor sensor for detecting target molecules and molecular change effects in protein recognition, analysis and quantification comprises a field effect transistor with a gate produced from SOI substrates
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
GB0211564D0 (en) 2002-05-21 2002-06-26 Tournaz Technology Ltd Reference circuit
AU2003245322A1 (en) 2002-05-29 2003-12-19 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Nanoscale ink-jet printing
US20040136866A1 (en) 2002-06-27 2004-07-15 Nanosys, Inc. Planar nanowire based sensor elements, devices, systems and methods for using and making same
KR100479128B1 (en) 2002-07-22 2005-03-28 학교법인 한양학원 Micro-magnetoelastic biosensor arry for detection of DNA hybridization and fabricating method thereof
TWI234584B (en) 2002-08-16 2005-06-21 Univ Nat Taiwan Normal Nucleic acid sequencing method
US20040197793A1 (en) 2002-08-30 2004-10-07 Arjang Hassibi Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
WO2004027024A2 (en) 2002-09-17 2004-04-01 Pharmacia Corporation Isolation of genetic molecules from a complex biological construct for use in genetic expression analysis
US7095010B2 (en) 2002-12-04 2006-08-22 California Institute Of Technology Silicon on insulator resonator sensors and modulators and method of operating the same
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
WO2004060044A2 (en) 2003-01-02 2004-07-22 Bioforce Nanosciences, Inc. Method and apparatus for molecular analysis in small sample volumes
ES2338654T5 (en) 2003-01-29 2017-12-11 454 Life Sciences Corporation Pearl emulsion nucleic acid amplification
CN103397082B (en) 2003-02-26 2017-05-31 考利达基因组股份有限公司 The random array DNA analysis carried out by hybridization
WO2004092708A2 (en) 2003-03-07 2004-10-28 University Of North Carolina At Chapel Hill Methods for the electrochemical detection of target compounds
US20050136419A1 (en) 2003-03-28 2005-06-23 The Regents Of The University Of California Method and apparatus for nanogap device and array
DE10319045A1 (en) 2003-04-25 2004-12-09 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Device and method for processing liquids containing biopolymers
US7291496B2 (en) 2003-05-22 2007-11-06 University Of Hawaii Ultrasensitive biochemical sensor
DK1633888T3 (en) 2003-06-17 2009-07-13 Keygene Nv Means and method for detecting target nucleotide sequences using ligation assays with improved oligonucleotide probe pairs
US7670810B2 (en) 2003-06-20 2010-03-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005019798A2 (en) 2003-08-13 2005-03-03 The Regents Of The University Of Michigan Biochemical sensors with micro-resonators
US20050084980A1 (en) 2003-10-17 2005-04-21 Intel Corporation Method and device for detecting a small number of molecules using surface-enhanced coherant anti-stokes raman spectroscopy
US20100000881A1 (en) 2003-10-30 2010-01-07 North Carolina State University Electrochemical detection of nucleic acid hybridization
WO2005043160A2 (en) 2003-10-31 2005-05-12 University Of Hawaii Ultrasensitive biochemical sensing platform
US7294246B2 (en) 2003-11-06 2007-11-13 3M Innovative Properties Company Electrode for electrochemical sensors
WO2005108612A2 (en) 2003-11-28 2005-11-17 Genorx, Inc. Nanoscale biosensor device, system and technique
EP2287341B1 (en) 2003-12-01 2013-02-13 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US20050129526A1 (en) 2003-12-10 2005-06-16 Dukhin Andrei S. Method of using unbalanced alternating electric field in microfluidic devices
JP3903183B2 (en) 2004-02-03 2007-04-11 独立行政法人物質・材料研究機構 Gene detection field effect device and gene polymorphism analysis method using the same
US20050218464A1 (en) 2004-03-18 2005-10-06 Holm-Kennedy James W Biochemical ultrasensitive charge sensing
US7238536B1 (en) 2004-03-22 2007-07-03 Florida State University Research Foundation, Inc. Controlled transport through multiple reversible interaction point membranes
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
ES2718482T3 (en) 2004-06-01 2019-07-02 Alere San Diego Inc Recombinase polymerase amplification kit
AU2005254984B2 (en) 2004-06-10 2008-10-16 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis
GB0413082D0 (en) 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
GB2416210B (en) 2004-07-13 2008-02-20 Christofer Toumazou Ion sensitive field effect transistors
US7888013B2 (en) 2004-08-27 2011-02-15 National Institute For Materials Science Method of analyzing DNA sequence using field-effect device, and base sequence analyzer
EP1788383B1 (en) * 2004-08-30 2017-04-05 Japan Science and Technology Agency Dna sensor
JP2008519265A (en) 2004-11-01 2008-06-05 メディミューン，インコーポレーテッド Ultra-high throughput capture lift screening method
WO2006050499A2 (en) 2004-11-03 2006-05-11 Third Wave Technologies, Inc. Single step detection assay
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
EP1831670A4 (en) 2004-12-28 2010-09-15 Nanomix Inc Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences
EA011039B1 (en) 2004-12-30 2008-12-30 Медивир Аб Compounds useful in the treatment of hiv
AU2006210553A1 (en) 2005-02-01 2006-08-10 Ab Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US8940143B2 (en) 2007-06-29 2015-01-27 Intel Corporation Gel-based bio chip for electrochemical synthesis and electrical detection of polymers
EP2385140B1 (en) 2005-07-25 2014-07-16 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
CA2621146A1 (en) 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US8179296B2 (en) 2005-09-30 2012-05-15 The Massachusetts Institute Of Technology Digital readout method and apparatus
EP1929269A2 (en) 2005-09-30 2008-06-11 Caliper Life Sciences, Inc. Microfluidic device for purifying a biological component using magnetic beads
US7615382B2 (en) 2005-11-09 2009-11-10 The Board Of Trustees Of The Leland Stanford Junior University Magnetic sifter
CA2637617C (en) 2006-02-16 2018-09-18 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
WO2007107910A1 (en) 2006-03-21 2007-09-27 Koninklijke Philips Electronics N. V. Microelectronic device with field electrodes
CN101405083A (en) 2006-03-21 2009-04-08 皇家飞利浦电子股份有限公司 Microelectronic device with field electrodes
JP2009535053A (en) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド Recombinase polymerase amplification
US20100105035A1 (en) 2006-11-22 2010-04-29 Syed Anwar Hashsham Electroluminescent-based fluorescence detection device
US7989396B2 (en) 2006-12-05 2011-08-02 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule immobilization on biosensors
US9188594B2 (en) 2006-12-06 2015-11-17 Yale University Nanoelectronic-enzyme linked immunosorbent assay system and method
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP4134667A1 (en) 2006-12-14 2023-02-15 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
JP5396281B2 (en) 2007-01-24 2014-01-22 アレイオミックス，インコーポレイテッド Method for arranging microdevices, magnetic arrangement apparatus used in the method, microdevice product used in the method, and system for arranging microdevices
US8315817B2 (en) 2007-01-26 2012-11-20 Illumina, Inc. Independently removable nucleic acid sequencing system and method
CA2676570C (en) 2007-01-26 2016-05-03 Illumina, Inc. Nucleic acid sequencing system and method
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US7731414B2 (en) 2007-02-08 2010-06-08 Instrumentation Laboratory Company Reagent cartridge mixing tube
US9063117B2 (en) 2007-02-21 2015-06-23 Paul L. Gourley Micro-optical cavity with fluidic transport chip for bioparticle analysis
JP4945279B2 (en) 2007-03-23 2012-06-06 株式会社日立製作所 DNA analysis method and analyzer
JP2008245612A (en) 2007-03-30 2008-10-16 Hitachi Ltd Method and device for preparing sample
US9034637B2 (en) 2007-04-25 2015-05-19 Nxp, B.V. Apparatus and method for molecule detection using nanopores
ATE554859T1 (en) 2007-05-24 2012-05-15 Univ California INTEGRATED FLUIDIC DEVICES WITH MAGNETIC SORTING
JP2009002808A (en) 2007-06-21 2009-01-08 Hitachi Ltd System and method for measuring dna
JP4876031B2 (en) 2007-06-22 2012-02-15 株式会社日立製作所 Analysis equipment
AU2008276308A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
JP2010534474A (en) 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Molecular redundant sequencing
EP2212683A4 (en) 2007-10-17 2011-08-31 Advanced Liquid Logic Inc Manipulation of beads in droplets
CN101918590B (en) 2007-12-10 2013-03-27 高晓莲 Sequencing of nucleic acids
CN101896624A (en) 2007-12-13 2010-11-24 Nxp股份有限公司 A biosensor device and a method of sequencing biological particles
US8372585B2 (en) 2007-12-31 2013-02-12 Intel Corporation Electronic sensing for nucleic acid sequencing
US8067176B2 (en) 2008-01-25 2011-11-29 Shimadzu Corporation Microchemistry reaction method
US8173080B2 (en) 2008-02-14 2012-05-08 Illumina, Inc. Flow cells and manifolds having an electroosmotic pump
US9017973B2 (en) * 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
GB0805814D0 (en) 2008-03-31 2008-04-30 Cambridge Entpr Ltd Biosensor for analyte detection
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
US8940142B2 (en) 2008-05-05 2015-01-27 The Regents Of The University Of California Functionalized nanopipette biosensor
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
AU2009249403A1 (en) 2008-05-19 2009-11-26 The University Of North Carolina At Chapel Hill Methods and compositions comprising novel cationic lipids
LT2660336T (en) 2008-06-11 2017-10-10 Orion Pharma (Uk) Limited Isothermal nucleic acid amplification
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
ES2532632T3 (en) 2008-06-30 2015-03-30 Life Technologies Corporation Direct amplification method from raw nucleic acid samples
CN102144037A (en) 2008-07-07 2011-08-03 牛津纳米孔技术有限公司 Base-detecting pore
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
WO2010026488A2 (en) 2008-09-03 2010-03-11 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
WO2017120148A1 (en) * 2016-01-04 2017-07-13 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2010033193A2 (en) 2008-09-16 2010-03-25 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
US8945912B2 (en) 2008-09-29 2015-02-03 The Board Of Trustees Of The University Of Illinois DNA sequencing and amplification systems using nanoscale field effect sensor arrays
US8392126B2 (en) 2008-10-03 2013-03-05 Illumina, Inc. Method and system for determining the accuracy of DNA base identifications
EP2350652A2 (en) 2008-10-10 2011-08-03 Cnrs-Dae Cell sorting device
JP2012506557A (en) 2008-10-22 2012-03-15 ライフ テクノロジーズ コーポレーション Integrated sensor arrays for biological and chemical analysis
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8546128B2 (en) 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100112588A1 (en) 2008-11-04 2010-05-06 Caerus Molecular Diagnostics, Inc. Methods for sanger sequencing using particle associated clonal amplicons and highly parallel electrophoretic size-based separation
EP2379748A4 (en) 2008-12-23 2012-08-29 Illumina Inc Multibase delivery for long reads in sequencing by synthesis protocols
US8563240B2 (en) 2008-12-31 2013-10-22 Intel Corporation Nucleic acid sequencing and electronic detection
CN103952482A (en) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 Multi-primer amplification method for barcoding of target nucleic acids
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP3663750B1 (en) 2009-05-29 2021-11-03 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
EP3360974A1 (en) 2009-06-05 2018-08-15 Alere San Diego, Inc. Recombinase polymerase amplification reagents
CN107739750A (en) 2009-09-25 2018-02-27 艾利尔圣迭戈公司 The detection of nucleic acid in thick matrix
US8962279B2 (en) 2009-12-30 2015-02-24 Intel Corporation Solid-phase chelators and electronic biosensors
EP3943920B1 (en) 2010-02-19 2024-04-03 Pacific Biosciences Of California, Inc. Integrated analytical system and method for fluorescence measurement
WO2011106556A2 (en) 2010-02-24 2011-09-01 Life Technologies Corporation Systems and methods for adaptive recursive sequencing
US8524064B2 (en) 2010-04-22 2013-09-03 Lawrence Livermore National Security, Llc Three dimensional microelectrode system for dielectrophoresis
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US9309569B2 (en) * 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
EP3572528A1 (en) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
WO2012047889A2 (en) 2010-10-04 2012-04-12 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
EP3564392B1 (en) 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130090860A1 (en) 2010-12-30 2013-04-11 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
GB2488600B (en) 2011-03-04 2013-05-29 Hm Technology Internat Ltd A force sensor
EP3978620A1 (en) 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
US20120264617A1 (en) 2011-04-14 2012-10-18 Pettit John W Dna sequencing employing nanomaterials
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2012149438A1 (en) 2011-04-28 2012-11-01 Life Technologies Corporation Methods and compositions for multiplex pcr
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CA2837486C (en) 2011-05-27 2020-10-27 Genapsys, Inc. Systems and methods for nucleic acid sequencing
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
US9689835B2 (en) 2011-10-31 2017-06-27 Taiwan Semiconductor Manufacturing Company, Ltd. Amplified dual-gate bio field effect transistor
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
US8795605B2 (en) 2012-01-12 2014-08-05 Fred C. Senftleber Apparatus and methods for transferring materials between locations possessing different cross-sectional areas with minimal band spreading and dispersion due to unequal path-lengths
EP3591408A1 (en) 2012-02-10 2020-01-08 The University of North Carolina at Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
US8906320B1 (en) 2012-04-16 2014-12-09 Illumina, Inc. Biosensors for biological or chemical analysis and systems and methods for same
US9494554B2 (en) 2012-06-15 2016-11-15 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CN110777195A (en) 2012-07-13 2020-02-11 生命技术公司 Human identity recognition using a set of SNPs
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
ES2750635T3 (en) 2012-10-16 2020-03-26 Abbott Lab Method of increasing the length of Debye on the surface of a sensor in a sample solution and the corresponding computer-readable storage medium
CN102980922A (en) 2012-11-21 2013-03-20 济南大学 Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening
US10106842B2 (en) * 2013-03-15 2018-10-23 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
EP2994544B1 (en) * 2013-05-06 2019-10-02 Pacific Biosciences Of California, Inc. Real-time electronic sequencing
US20150316502A1 (en) * 2013-12-03 2015-11-05 FemtoDx Debye length modulation
US10125393B2 (en) * 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3117221B1 (en) 2014-03-13 2020-09-09 Genapsys Inc. Microfluidic devices and methods for sample preparation and analysis
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US10605766B2 (en) * 2014-07-15 2020-03-31 Illumina, Inc. Biochemically activated electronic device
WO2016127077A2 (en) * 2015-02-06 2016-08-11 Genapsys, Inc Systems and methods for detection and analysis of biological species
EP3332032B1 (en) * 2015-08-06 2023-12-27 Pacific Biosciences Of California, Inc. Integrated circuits for selectively addressing sparsely arranged electronic measurement devices
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
MX2020003113A (en) 2017-09-21 2020-09-07 Genapsys Inc Systems and methods for nucleic acid sequencing.

Also Published As

Publication number Publication date
US20190256903A1 (en) 2019-08-22
JP2023040054A (en) 2023-03-22
EP3684951A1 (en) 2020-07-29
MX2020003113A (en) 2020-09-07
CN111566224A (en) 2020-08-21
US20220186303A1 (en) 2022-06-16
CA3076378A1 (en) 2019-03-28
US10900075B2 (en) 2021-01-26
IL273426A (en) 2020-05-31
EP3684951A4 (en) 2021-06-16
WO2019060628A1 (en) 2019-03-28
JP2021500858A (en) 2021-01-14

Similar Documents

Publication Publication Date Title
HK1250051A1 (en) Nucleic acid sequencing methods and systems
EP3488017A4 (en) Systems and methods for nucleic acid sequencing
IL259736A (en) Methods and systems for nucleic acid analysis
HK1250182A1 (en) Systems and methods for analyzing nucleic acid
IL253196A0 (en) Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
HK1253013A1 (en) Systems and methods for barcoding nucleic acid
IL249461B (en) Processes and systems for nucleic acid sequence assembly
IL267424A (en) Methods and systems for analyzing nucleic acid molecules
EP3359691A4 (en) Devices, systems and methods for nucleic acid sequencing
GB201620692D0 (en) Sequencing systems and methods
IL273426A (en) Systems and methods for nucleic acid sequencing
HK1223972A1 (en) Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dna
EP3472359A4 (en) Nucleic acid sequencing
EP3194627C0 (en) Methods and systems for analyzing nucleic acid sequencing data
EP3332033A4 (en) Single-molecule nanofet sequencing systems and methods
EP3294122A4 (en) Ultrasound sequencing system and method
EP3371194A4 (en) Protein sequencing methods and reagents
ZA201901372B (en) Method and system for crispr-based library preparation and sequencing
GB201701262D0 (en) Nucleic acid detection method
GB2589159B (en) Nucleic acid sequencing apparatus
EP3347714A4 (en) Methods and systems for multiplex quantitative nucleic acid amplification
IL264513A (en) Device and method for nucleic acid manipulation
EP3508587A4 (en) Dna sequencing method
EP3277845A4 (en) Beads for nucleic acid sequencing
PL3663407T3 (en) Nucleic acid sequencing method