HK1223972A1 - Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dna - Google Patents
Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dnaInfo
- Publication number
- HK1223972A1 HK1223972A1 HK16112145.1A HK16112145A HK1223972A1 HK 1223972 A1 HK1223972 A1 HK 1223972A1 HK 16112145 A HK16112145 A HK 16112145A HK 1223972 A1 HK1223972 A1 HK 1223972A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- dna
- crispr
- cas9
- assembly
- assembly method
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/102—Plasmid DNA for yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/10—Vectors comprising a non-peptidic targeting moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510036972 | 2015-01-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1223972A1 true HK1223972A1 (en) | 2017-08-11 |
Family
ID=56987355
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK16112145.1A HK1223972A1 (en) | 2015-01-23 | 2016-10-20 | Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dna |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN105821072A (en) |
HK (1) | HK1223972A1 (en) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
CN108513575A (en) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | Nucleobase editing machine and application thereof |
KR20230095129A (en) | 2016-08-03 | 2023-06-28 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Adenosine nucleobase editors and uses thereof |
EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
CN107784200B (en) * | 2016-08-26 | 2020-11-06 | 深圳华大生命科学研究院 | Method and device for screening novel CRISPR-Cas system |
CN107779466B (en) * | 2016-08-31 | 2021-01-08 | 上海交通大学 | Methods for gene disruption in higher fungi |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
JP2020510439A (en) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Base-editing factor from cytosine to guanine |
US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
CN108728468B (en) * | 2017-04-21 | 2022-02-01 | 南通大学 | Method and vector for cloning target DNA fragment |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
CN108486158B (en) * | 2017-12-06 | 2020-11-17 | 上海泽因生物科技有限公司 | Construction method of gene detection standard substance based on yeast cells and kit thereof |
CN109868288A (en) * | 2019-03-15 | 2019-06-11 | 张力 | Cas9 transcription templates DNA and Plasmid DNA and in-vitro transcription method for drosophila CRISPR transgenosis |
WO2020191249A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
CN110322927B (en) * | 2019-08-02 | 2021-04-09 | 中南大学 | CRISPR (clustered regularly interspaced short palindromic repeats) induced RNA (ribonucleic acid) library design method |
DE112021002672T5 (en) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EDIT BOTH STRANDS SIMULTANEOUSLY OF A DOUBLE STRANDED NUCLEOTIDE TARGET SEQUENCE |
CN115807021A (en) * | 2022-08-01 | 2023-03-17 | 深圳大学 | Method for synthesizing, assembling and testing functions of artificial chloroplast genome of chlamydomonas reinhardtii |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013181440A1 (en) * | 2012-05-30 | 2013-12-05 | Baylor College Of Medicine | Supercoiled minivectors as a tool for dna repair, alteration and replacement |
BR112015009931A2 (en) * | 2012-10-31 | 2017-12-05 | Two Blades Found | gene, protein, nucleic acid molecule, vector, host cell, methods for in vitro preparation of a mutant gene and for generating a plant, mutant plant, product, mutant plant seed, antibody, use of an antibody, gene probe, pair of primer oligonucleotides, and use of genetic probe |
US9447422B2 (en) * | 2012-12-06 | 2016-09-20 | Synthetic Genomics, Inc. | Autonomous replication sequences and episomal DNA molecules |
CN103343120B (en) * | 2013-07-04 | 2015-03-04 | 中国科学院遗传与发育生物学研究所 | Wheat genome site-specific modification method |
CN103382468B (en) * | 2013-07-04 | 2015-04-29 | 中国科学院遗传与发育生物学研究所 | Site-directed modification method of rice genome |
CN104109687A (en) * | 2014-07-14 | 2014-10-22 | 四川大学 | Construction and application of Zymomonas mobilis CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-association proteins)9 system |
-
2016
- 2016-01-22 CN CN201610044240.0A patent/CN105821072A/en active Pending
- 2016-10-20 HK HK16112145.1A patent/HK1223972A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN105821072A (en) | 2016-08-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HK1223972A1 (en) | Crispr-cas9 system for dna assembly and dna assembly method dna crispr-cas9 dna | |
HK1250182A1 (en) | Systems and methods for analyzing nucleic acid | |
ZA201900536B (en) | Blockchain-implemented method and system | |
ZA201900509B (en) | Blockchain-implemented method and system | |
IL259736A (en) | Methods and systems for nucleic acid analysis | |
GB201719944D0 (en) | Parking-lot-navigation system and method | |
GB201713365D0 (en) | Order pushing method and system | |
PT3260813T (en) | Ranging system and ranging method | |
PL3233370T3 (en) | Modular vehicle assembly system and method | |
GB201516860D0 (en) | Fluid method and system | |
ZA201705408B (en) | Control system and control method | |
ZA201800288B (en) | System and method for tokenization | |
GB201516863D0 (en) | Fluid method and system | |
GB201516864D0 (en) | Fluid method and system | |
GB201522623D0 (en) | Guidance system and method for providing guidance | |
GB2542548B (en) | System and method | |
GB201510480D0 (en) | System and method | |
SG11201708785XA (en) | Tele-health and tele-medical facilitation system and method thereof | |
SG11201706222PA (en) | Attaching System And Attaching Method | |
GB201604012D0 (en) | Refridgeration system and method | |
GB201515115D0 (en) | System and method | |
GB201803529D0 (en) | Radio-station-recommendation system and method | |
GB2590335B (en) | Control system and method | |
GB201620926D0 (en) | Method and system | |
GB201613049D0 (en) | Connection system and method |