DE60112746D1 - Kälteempfindliche dna-polymerasemutanten - Google Patents

Kälteempfindliche dna-polymerasemutanten

Info

Publication number
DE60112746D1
DE60112746D1 DE60112746T DE60112746T DE60112746D1 DE 60112746 D1 DE60112746 D1 DE 60112746D1 DE 60112746 T DE60112746 T DE 60112746T DE 60112746 T DE60112746 T DE 60112746T DE 60112746 D1 DE60112746 D1 DE 60112746D1
Authority
DE
Germany
Prior art keywords
dna polymerases
mutation
mutants
cold
compared
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60112746T
Other languages
English (en)
Other versions
DE60112746T2 (de
Inventor
M Barnes
B Kermekchiev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
St Louis University
Washington University in St Louis WUSTL
Original Assignee
St Louis University
Washington University in St Louis WUSTL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Louis University, Washington University in St Louis WUSTL filed Critical St Louis University
Publication of DE60112746D1 publication Critical patent/DE60112746D1/de
Application granted granted Critical
Publication of DE60112746T2 publication Critical patent/DE60112746T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE60112746T 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten Expired - Lifetime DE60112746T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US587856 2000-06-06
US09/587,856 US6214557B1 (en) 2000-06-06 2000-06-06 Cold sensitive mutant DNA polymerases
PCT/US2001/018236 WO2001094562A2 (en) 2000-06-06 2001-06-05 Cold sensitive mutant dna polymerases

Publications (2)

Publication Number Publication Date
DE60112746D1 true DE60112746D1 (de) 2005-09-22
DE60112746T2 DE60112746T2 (de) 2006-05-18

Family

ID=24351474

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60112746T Expired - Lifetime DE60112746T2 (de) 2000-06-06 2001-06-05 Kälteempfindliche dna-polymerasemutanten

Country Status (7)

Country Link
US (3) US6214557B1 (de)
EP (1) EP1287146B1 (de)
JP (1) JP2003535587A (de)
AT (1) ATE302279T1 (de)
AU (2) AU2001266726B2 (de)
DE (1) DE60112746T2 (de)
WO (1) WO2001094562A2 (de)

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AU785425B2 (en) * 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
AU2002327421A1 (en) * 2001-08-02 2003-02-17 Wayne M. Barnes Magnesium precipitate hot start method for molecular manipulation of nucleic acids
US20030108919A1 (en) * 2001-09-05 2003-06-12 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
US6740510B2 (en) * 2001-09-05 2004-05-25 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
US6898531B2 (en) 2001-09-05 2005-05-24 Perlegen Sciences, Inc. Algorithms for selection of primer pairs
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
JP2007512020A (ja) * 2003-11-26 2007-05-17 エッペンドルフ アクチェンゲゼルシャフト 染色体外核酸のインビトロ増幅のための方法及び組成物
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
EP2208796B1 (de) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptide mit Nukleinsäurebindungsaktivität und Zusammensetzungen und Verfahren zur Nukleinsäurenamplifikation
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007031952A2 (en) * 2005-09-13 2007-03-22 John Mastin Method of preparing upholstery for installation on an article of furniture
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
WO2012139748A1 (en) 2011-04-11 2012-10-18 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2012173905A1 (en) * 2011-06-16 2012-12-20 The Regents Of The Unversity Of California Salt-tolerant dna polymerases
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
LT2742151T (lt) 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
EP2788481B1 (de) 2011-12-08 2018-03-07 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
CN104487596B (zh) 2012-06-14 2020-03-13 生命技术公司 用于聚合酶链式反应(pcr)的新型组合物、方法和试剂盒
CN103509767B (zh) * 2012-06-27 2016-08-03 菲鹏生物股份有限公司 一种突变型Taq酶及其制备方法
JP6074036B2 (ja) * 2012-08-08 2017-02-01 ウニベルジテーツ コンスタンツ 拡大された基質範囲を有する新規のｄｎａポリメラーゼ
CN105039278B (zh) * 2015-06-17 2018-09-11 菲鹏生物股份有限公司 突变型Taq DNA聚合酶及其制备方法和应用
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
CN111826366B (zh) * 2020-06-05 2022-08-02 广州英赞生物科技有限公司 一种直扩型热启动dna聚合酶及其制备方法与应用
CN113257347B (zh) * 2021-05-14 2022-02-11 温州谱希医学检验实验室有限公司 注释后的突变检测结果文件的数据处理方法及相关设备

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554101A (en) 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
DK2080813T3 (da) * 1995-06-07 2011-03-28 Gilead Sciences Inc Nukleinsyreligander, som binder til og inhiberer DNA-polymeraser
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US6395524B2 (en) * 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
AU743025B2 (en) * 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes

Also Published As

Publication number Publication date
EP1287146B1 (de) 2005-08-17
AU2001266726B2 (en) 2006-07-13
EP1287146A2 (de) 2003-03-05
ATE302279T1 (de) 2005-09-15
AU6672601A (en) 2001-12-17
WO2001094562A2 (en) 2001-12-13
JP2003535587A (ja) 2003-12-02
US6333159B1 (en) 2001-12-25
WO2001094562A3 (en) 2002-05-30
US6214557B1 (en) 2001-04-10
US6316202B1 (en) 2001-11-13
US20020004203A1 (en) 2002-01-10
DE60112746T2 (de) 2006-05-18

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