DE553264T1 - Thermostabile dna polymerase. - Google Patents

Thermostabile dna polymerase.

Info

Publication number
DE553264T1
DE553264T1 DE91920050T DE91920050T DE553264T1 DE 553264 T1 DE553264 T1 DE 553264T1 DE 91920050 T DE91920050 T DE 91920050T DE 91920050 T DE91920050 T DE 91920050T DE 553264 T1 DE553264 T1 DE 553264T1
Authority
DE
Germany
Prior art keywords
dna polymerase
acid sequence
amino acid
taq dna
terminus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE91920050T
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English (en)
Inventor
Wayne M Barnes
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Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of DE553264T1 publication Critical patent/DE553264T1/de
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (6)

Patentansprüche:
1. Vektor, der eine Nucleinsaure aufweist, die für eine DNA-Polymerase codiert, deren Aminosäurenseqenz im wesentlichen die Aminosäurensequenz der Taq DNA-Polymerase von Termus aauaticus umfasst, wobei an dem N-Ende 235 Aminosäuren der Taq DNA-Polymerase fehlen.
2. Der Vektor nach Anspruch 1, bei dem die Nucleinsaure mit der identisch ist, die in dem Plasmid pWB2 5 3 vorliegt, das in der unter der ATCC No. 68431 hinterlegten Wirtszelle vorhanden ist.
3. Wirtszelle, die einen Vektor enthält, die für eine DNA-Polymerase codiert, deren Aminosäurenseqenz im wesentlichen die Aminosäurensequenz der Taq DNA-Polymerase von Termus aquaticus umfasst, wobei an dem N-Ende 235 Aminosäuren der Taq DNA-Polymerase fehlen.
4. Wirtszelle nach Anspruch 3. die unter der ATCC No. 68431 hintelegt ist.
5. Gereinigte DNA-Polymerase, die eine Aminosäurensequenz aufweist, die im wesentlichen die Aminosäurensequenz der Taq DNA-Polymerase von Thermus acuaticus umfaßt, wobei an N-Ende 235 Aminosäuren der Taq DNA-Polymerase fehlen.
6. Gereinigte DNA-Polymerase nach Anspruch 5, die als homogene Zubereitung vorliegt.
DE91920050T 1990-10-05 1991-09-27 Thermostabile dna polymerase. Pending DE553264T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59463790A 1990-10-05 1990-10-05
PCT/US1991/007084 WO1992006188A2 (en) 1990-10-05 1991-09-27 Thermostable dna polymerase

Publications (1)

Publication Number Publication Date
DE553264T1 true DE553264T1 (de) 1994-04-28

Family

ID=24379740

Family Applications (1)

Application Number Title Priority Date Filing Date
DE91920050T Pending DE553264T1 (de) 1990-10-05 1991-09-27 Thermostabile dna polymerase.

Country Status (6)

Country Link
US (1) US5616494A (de)
EP (1) EP0553264A4 (de)
JP (1) JPH06502303A (de)
AU (1) AU8906091A (de)
DE (1) DE553264T1 (de)
WO (1) WO1992006188A2 (de)

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Also Published As

Publication number Publication date
EP0553264A1 (de) 1993-08-04
WO1992006188A2 (en) 1992-04-16
WO1992006188A3 (en) 1992-10-29
JPH06502303A (ja) 1994-03-17
EP0553264A4 (en) 1994-07-13
AU8906091A (en) 1992-04-28
US5616494A (en) 1997-04-01

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