EP4274894A2 - Prime-editor-varianten, konstrukte und verfahren zur verbesserung der prime-editierungseffizienz und -präzision - Google Patents
Prime-editor-varianten, konstrukte und verfahren zur verbesserung der prime-editierungseffizienz und -präzisionInfo
- Publication number
- EP4274894A2 EP4274894A2 EP22702103.7A EP22702103A EP4274894A2 EP 4274894 A2 EP4274894 A2 EP 4274894A2 EP 22702103 A EP22702103 A EP 22702103A EP 4274894 A2 EP4274894 A2 EP 4274894A2
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Y—ENZYMES
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- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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| US202163136194P | 2021-01-11 | 2021-01-11 | |
| US202163176180P | 2021-04-16 | 2021-04-16 | |
| US202163176202P | 2021-04-16 | 2021-04-16 | |
| US202163194865P | 2021-05-28 | 2021-05-28 | |
| US202163194913P | 2021-05-28 | 2021-05-28 | |
| US202163231230P | 2021-08-09 | 2021-08-09 | |
| US202163255897P | 2021-10-14 | 2021-10-14 | |
| PCT/US2022/012054 WO2022150790A2 (en) | 2021-01-11 | 2022-01-11 | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
Publications (1)
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| EP4274894A2 true EP4274894A2 (de) | 2023-11-15 |
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|---|---|
| EP (1) | EP4274894A2 (de) |
| JP (1) | JP2024503437A (de) |
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| WO2023096847A2 (en) * | 2021-11-24 | 2023-06-01 | Prime Medicine, Inc. | Methods and compositions for inhibiting mismatch repair |
| US20240052370A1 (en) * | 2022-07-27 | 2024-02-15 | Inscripta, Inc. | Modulating cellular repair mechanisms for genomic editing |
| WO2024077267A1 (en) | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Prime editing methods and compositions for treating triplet repeat disorders |
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