DK3212778T3 - Fremgangsmåder og sammensætninger til stabilisering af trans-splejsning af intein-modificerede proteaser - Google Patents

Fremgangsmåder og sammensætninger til stabilisering af trans-splejsning af intein-modificerede proteaser Download PDF

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Publication number
DK3212778T3
DK3212778T3 DK15854157T DK15854157T DK3212778T3 DK 3212778 T3 DK3212778 T3 DK 3212778T3 DK 15854157 T DK15854157 T DK 15854157T DK 15854157 T DK15854157 T DK 15854157T DK 3212778 T3 DK3212778 T3 DK 3212778T3
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DK
Denmark
Prior art keywords
intein
splicing
compositions
methods
modified proteases
Prior art date
Application number
DK15854157T
Other languages
English (en)
Inventor
Michael R Raab
Binzhang Shen
Gabor Lazar
Original Assignee
Agrivida Inc
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Publication of DK3212778T3 publication Critical patent/DK3212778T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
DK15854157T 2014-10-28 2015-10-28 Fremgangsmåder og sammensætninger til stabilisering af trans-splejsning af intein-modificerede proteaser DK3212778T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462069653P 2014-10-28 2014-10-28
PCT/US2015/057862 WO2016069774A1 (en) 2014-10-28 2015-10-28 Methods and compositions for stabilizing trans-splicing intein modified proteases

Publications (1)

Publication Number Publication Date
DK3212778T3 true DK3212778T3 (da) 2019-11-04

Family

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Family Applications (1)

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DK15854157T DK3212778T3 (da) 2014-10-28 2015-10-28 Fremgangsmåder og sammensætninger til stabilisering af trans-splejsning af intein-modificerede proteaser

Country Status (7)

Country Link
US (2) US10731143B2 (da)
EP (1) EP3212778B1 (da)
CN (1) CN107075491B (da)
BR (1) BR112017008938B1 (da)
CA (1) CA2963148A1 (da)
DK (1) DK3212778T3 (da)
WO (1) WO2016069774A1 (da)

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Publication number Publication date
US10731143B2 (en) 2020-08-04
CN107075491B (zh) 2021-07-06
US11066657B2 (en) 2021-07-20
CA2963148A1 (en) 2016-05-06
US20200308564A1 (en) 2020-10-01
EP3212778A1 (en) 2017-09-06
CN107075491A (zh) 2017-08-18
WO2016069774A1 (en) 2016-05-06
BR112017008938A2 (pt) 2018-01-16
EP3212778A4 (en) 2018-05-02
BR112017008938B1 (pt) 2023-09-26
EP3212778B1 (en) 2019-08-28
US20180298368A1 (en) 2018-10-18

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