WO2004016811A3 - Methods and kit for genes shuffling using tagged primers - Google Patents

Methods and kit for genes shuffling using tagged primers Download PDF

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Publication number
WO2004016811A3
WO2004016811A3 PCT/DK2003/000545 DK0300545W WO2004016811A3 WO 2004016811 A3 WO2004016811 A3 WO 2004016811A3 DK 0300545 W DK0300545 W DK 0300545W WO 2004016811 A3 WO2004016811 A3 WO 2004016811A3
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WO
WIPO (PCT)
Prior art keywords
methods
strands
primers
cleaved
genes
Prior art date
Application number
PCT/DK2003/000545
Other languages
French (fr)
Other versions
WO2004016811A2 (en
Inventor
Jens Nielsen
Uffe Mortensen
Ana Claudia Cantisani Borges
Michael Lynge Nielsen
Original Assignee
Univ Danmarks Tekniske
Jens Nielsen
Uffe Mortensen
Ana Claudia Cantisani Borges
Michael Lynge Nielsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Danmarks Tekniske, Jens Nielsen, Uffe Mortensen, Ana Claudia Cantisani Borges, Michael Lynge Nielsen filed Critical Univ Danmarks Tekniske
Priority to AU2003250811A priority Critical patent/AU2003250811A1/en
Publication of WO2004016811A2 publication Critical patent/WO2004016811A2/en
Publication of WO2004016811A3 publication Critical patent/WO2004016811A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to methods and kits for gene shuffling, as well as to shuffled genes. In particular, the methods comprise amplification of the sequences to be shuffled using affinity tagged primers, separation of the strands of the amplified sequences using the affinity tag and cleavage of the strands. The strands may be cleaved as single stranded nucleic acids or heteroduplexes may be formed from the strands and the double stranded heteroduplexes may be cleaved. Shuffled genes may be obtained by using the cleaved fragments as both primers and templates in a nucleotide polymerisation reaction, for example PCR. The kits of the present invention comprises primers and enzymes suitable for perfoming the methods.
PCT/DK2003/000545 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers WO2004016811A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003250811A AU2003250811A1 (en) 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40413202P 2002-08-19 2002-08-19
US60/404,132 2002-08-19

Publications (2)

Publication Number Publication Date
WO2004016811A2 WO2004016811A2 (en) 2004-02-26
WO2004016811A3 true WO2004016811A3 (en) 2004-03-25

Family

ID=31888328

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000545 WO2004016811A2 (en) 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers

Country Status (2)

Country Link
AU (1) AU2003250811A1 (en)
WO (1) WO2004016811A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090162845A1 (en) 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
EP2397548A1 (en) * 2010-06-18 2011-12-21 Hyglos Invest GmbH Methods of generating and screening for lytic chimeric polypeptides
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
EP3265590B1 (en) * 2015-03-06 2019-01-02 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US11661597B2 (en) 2015-04-15 2023-05-30 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
CN106795569B (en) 2015-07-07 2017-12-05 上海真固生物科技有限公司 The method for reducing primer dimer amplification

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032845A1 (en) * 1997-01-24 1998-07-30 Bioinvent International Ab A method for in vitro molecular evolution of protein function
WO1998041653A1 (en) * 1997-03-18 1998-09-24 Novo Nordisk A/S An in vitro method for construction of a dna library
WO2002024953A1 (en) * 2000-09-21 2002-03-28 Merck & Co., Inc. Method for generating recombinant polynucleotides
WO2002048351A2 (en) * 2000-12-12 2002-06-20 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
WO2002061142A2 (en) * 2001-01-31 2002-08-08 Large Scale Biology Corporation Methods for homology-driven reassembly of nucleic acid sequences
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032845A1 (en) * 1997-01-24 1998-07-30 Bioinvent International Ab A method for in vitro molecular evolution of protein function
WO1998041653A1 (en) * 1997-03-18 1998-09-24 Novo Nordisk A/S An in vitro method for construction of a dna library
WO2002024953A1 (en) * 2000-09-21 2002-03-28 Merck & Co., Inc. Method for generating recombinant polynucleotides
WO2002048351A2 (en) * 2000-12-12 2002-06-20 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
WO2002061142A2 (en) * 2001-01-31 2002-08-08 Large Scale Biology Corporation Methods for homology-driven reassembly of nucleic acid sequences
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KIKUCHI M ET AL: "An effective family shuffling method using single-stranded DNA.", GENE. NETHERLANDS 8 FEB 2000, vol. 243, no. 1-2, 8 February 2000 (2000-02-08), pages 133 - 137, XP002265416, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
AU2003250811A1 (en) 2004-03-03
AU2003250811A8 (en) 2004-03-03
WO2004016811A2 (en) 2004-02-26

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