ATE194015T1 - Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung - Google Patents

Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

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Publication number
ATE194015T1
ATE194015T1 AT97122379T AT97122379T ATE194015T1 AT E194015 T1 ATE194015 T1 AT E194015T1 AT 97122379 T AT97122379 T AT 97122379T AT 97122379 T AT97122379 T AT 97122379T AT E194015 T1 ATE194015 T1 AT E194015T1
Authority
AT
Austria
Prior art keywords
dna polymerase
sequencing
thermostable dna
decoupling
direct
Prior art date
Application number
AT97122379T
Other languages
English (en)
Inventor
Svante Erik Prof Dr Paeaebo
Christian Alexis Dr Kilger
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE194015T1 publication Critical patent/ATE194015T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT97122379T 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung ATE194015T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19653494A DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung

Publications (1)

Publication Number Publication Date
ATE194015T1 true ATE194015T1 (de) 2000-07-15

Family

ID=7815667

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97122379T ATE194015T1 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

Country Status (5)

Country Link
US (3) US6225092B1 (de)
EP (1) EP0854196B1 (de)
JP (1) JPH10225297A (de)
AT (1) ATE194015T1 (de)
DE (2) DE19653494A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000516468A (ja) 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
AU743025B2 (en) * 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6605434B1 (en) * 1999-03-16 2003-08-12 Human Genome Sciences, Inc. Direct bacterial lysate sequencing
EP1144689A1 (de) * 1999-11-26 2001-10-17 Bioneer Corporation; Dna sequenzierungsmethode die verschiedene dna polymerasen verwendet und entsprechender kit
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6436641B1 (en) * 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
EP1472335A4 (de) * 2001-11-08 2005-12-28 Univ Johns Hopkins Verfahren und systeme zur nukleinsäuresequenzierung
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
US7776524B2 (en) * 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
AU2003269991A1 (en) * 2003-08-20 2005-03-10 Applera Corporation Polymerase compositions
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
US7981607B2 (en) 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
EP1634965B1 (de) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Echtzeit-PCR unter Zusatz von Pyrophosphatase
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP2432796B1 (de) 2009-05-21 2016-08-17 Siemens Healthcare Diagnostics Inc. Universaltags mit nicht-natürlichen nukleobasen
US9617588B2 (en) * 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
WO2014090836A1 (en) * 2012-12-13 2014-06-19 Roche Diagnostics Gmbh Dna polymerases with improved activity

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
EP0595982A4 (en) * 1991-07-24 1995-10-11 Univ Partnerships Pty Ltd Single step amplification and sequencing of nucleic acids
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification

Also Published As

Publication number Publication date
DE69702333D1 (de) 2000-07-27
EP0854196B1 (de) 2000-06-21
DE19653494A1 (de) 1998-06-25
US20020177129A1 (en) 2002-11-28
US20040146911A1 (en) 2004-07-29
DE69702333T2 (de) 2000-12-14
EP0854196A1 (de) 1998-07-22
JPH10225297A (ja) 1998-08-25
US6225092B1 (en) 2001-05-01

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