DE69835008D1 - Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) - Google Patents

Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)

Info

Publication number
DE69835008D1
DE69835008D1 DE69835008T DE69835008T DE69835008D1 DE 69835008 D1 DE69835008 D1 DE 69835008D1 DE 69835008 T DE69835008 T DE 69835008T DE 69835008 T DE69835008 T DE 69835008T DE 69835008 D1 DE69835008 D1 DE 69835008D1
Authority
DE
Germany
Prior art keywords
reverse transcriptase
pcr
methods
amplification
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69835008T
Other languages
English (en)
Other versions
DE69835008T2 (de
Inventor
E Lee
Ayoub Rashtchian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DE69835008D1 publication Critical patent/DE69835008D1/de
Publication of DE69835008T2 publication Critical patent/DE69835008T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69835008T 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) Expired - Lifetime DE69835008T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4262997P 1997-04-03 1997-04-03
US42629P 1997-04-03
PCT/US1998/006581 WO1998044161A1 (en) 1997-04-03 1998-04-03 Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)

Publications (2)

Publication Number Publication Date
DE69835008D1 true DE69835008D1 (de) 2006-08-03
DE69835008T2 DE69835008T2 (de) 2007-01-11

Family

ID=21922934

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69835008T Expired - Lifetime DE69835008T2 (de) 1997-04-03 1998-04-03 Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
DE69841990T Expired - Lifetime DE69841990D1 (de) 1997-04-03 1998-04-03 Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR)

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69841990T Expired - Lifetime DE69841990D1 (de) 1997-04-03 1998-04-03 Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR)

Country Status (8)

Country Link
US (6) US6495350B1 (de)
EP (3) EP2354251B1 (de)
AT (2) ATE486961T1 (de)
AU (1) AU7100198A (de)
DE (2) DE69835008T2 (de)
DK (1) DK1705253T3 (de)
ES (1) ES2355739T3 (de)
WO (1) WO1998044161A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593120B1 (en) 1994-04-01 2003-07-15 Gen-Probe Incorporated Recombinant DNA encoding a reverse transcriptase derived from moloney murine leukemia virus
ES2355739T3 (es) 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
JP2001523098A (ja) 1997-04-22 2001-11-20 ライフ テクノロジーズ，インコーポレイテッド 複数のサブユニットからなるａｓｌｖ逆転写酵素の生成のための方法
US20040185493A1 (en) * 1997-05-12 2004-09-23 Invitrogen Corporation Methods for production and purification of nucleic acid molecules
US7122304B2 (en) 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
CN1637146A (zh) * 1998-04-23 2005-07-13 宝生物工程株式会社 Dna的合成方法
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6300069B1 (en) 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
AU776129B2 (en) * 1999-05-21 2004-08-26 Invitrogen Corporation Compositions and methods for labeling of nucleic acid molecules
CA2460546A1 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
DK2143803T3 (en) * 2002-09-03 2016-08-01 Quanta Biosciences Inc Improved compositions and methods for cDNA synthesis
US20040157213A1 (en) * 2003-01-27 2004-08-12 Kennell John C. Fungal reverse transcriptases with enhanced capabilities
US20040259115A1 (en) * 2003-01-29 2004-12-23 Schuster David M. Methods and compositions for RNA detection and quantitation
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US20060003343A1 (en) * 2004-01-23 2006-01-05 Biomerieux, Inc. Primer and probe design for efficient amplification and detection of HCV 3' non-translating region
JP2008500818A (ja) * 2004-04-06 2008-01-17 ストラタジーン カリフォルニア 逆転写のための組成物および方法
US7566532B1 (en) 2004-07-02 2009-07-28 Quest Diagnostics Investments Incorporated Methods for detecting retroviruses
US7943294B2 (en) * 2004-07-30 2011-05-17 Hologic, Inc. Methods for detecting oncofetal fibronectin
EP1812604A4 (de) * 2004-11-03 2011-02-23 Third Wave Tech Inc Ein-schritt-nachweistest
EP1989318B1 (de) * 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaktionspufferzusammensetzung für die nukleinsäurereplikation mit gepackten dna-polymerasen
EP2019687B1 (de) 2006-03-29 2014-03-19 Merial Limited Impfstoff gegen streptokokken
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
JP5702148B2 (ja) * 2007-11-27 2015-04-15 バイオ−ラッド ラボラトリーズ，インコーポレイティド １ステップｒｔ−ｐｃｒの阻害低減
JP5279339B2 (ja) * 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
CA2718011C (en) 2008-03-15 2015-05-19 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reaction using footprint probes
US9005895B2 (en) 2010-06-21 2015-04-14 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
EP2705156B1 (de) * 2011-05-05 2015-08-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Lineare dna-amplifikation
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
WO2012170907A2 (en) * 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2559764A1 (de) 2011-08-17 2013-02-20 Qiagen GmbH Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer
WO2014063051A2 (en) 2012-10-18 2014-04-24 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof
EP3539944A1 (de) 2013-10-25 2019-09-18 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
WO2018005283A1 (en) 2016-06-27 2018-01-04 Dana-Farber Cancer Institute, Inc. Methods for measuring rna translation rates
WO2018136404A1 (en) * 2017-01-19 2018-07-26 Asuragen, Inc. Methods of rna amplification
WO2019118652A1 (en) 2017-12-12 2019-06-20 Essenlix Corporation Sample manipulation and assay with rapid temperature change
US11453907B2 (en) 2020-03-23 2022-09-27 The Broad Institute, Inc. Crispr effector system based coronavirus diagnostics
WO2022018685A1 (en) 2020-07-22 2022-01-27 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
AU2022305653A1 (en) 2021-06-30 2024-01-18 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5407800A (en) 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5618702A (en) 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
FR2690691B1 (fr) 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
JPH08131171A (ja) 1994-11-11 1996-05-28 Asahi Chem Ind Co Ltd 逆転写酵素の安定化組成物
EP0736608A1 (de) * 1995-04-08 1996-10-09 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
ES2355739T3 (es) 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
JP2001523098A (ja) * 1997-04-22 2001-11-20 ライフ テクノロジーズ，インコーポレイテッド 複数のサブユニットからなるａｓｌｖ逆転写酵素の生成のための方法
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6630333B1 (en) * 1999-03-23 2003-10-07 Invitrogen Corporation Substantially pure reverse transriptases and methods of production thereof
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US8278083B2 (en) 2004-03-22 2012-10-02 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inactivated influenza virus compositions

Also Published As

Publication number Publication date
US8372604B2 (en) 2013-02-12
DE69835008T2 (de) 2007-01-11
US20130164788A1 (en) 2013-06-27
US20150017688A1 (en) 2015-01-15
EP1705253A3 (de) 2006-12-27
US20030113712A1 (en) 2003-06-19
EP1705253A2 (de) 2006-09-27
AU7100198A (en) 1998-10-22
EP1705253B1 (de) 2010-11-03
EP0975806A1 (de) 2000-02-02
DE69841990D1 (de) 2010-12-16
ATE331040T1 (de) 2006-07-15
EP2354251A1 (de) 2011-08-10
US20110217735A1 (en) 2011-09-08
US20040219595A1 (en) 2004-11-04
ATE486961T1 (de) 2010-11-15
US9873901B2 (en) 2018-01-23
ES2355739T3 (es) 2011-03-30
US7883871B2 (en) 2011-02-08
DK1705253T3 (da) 2011-02-21
WO1998044161A1 (en) 1998-10-08
EP0975806A4 (de) 2004-10-06
US6767724B2 (en) 2004-07-27
EP2354251B1 (de) 2014-10-15
US6495350B1 (en) 2002-12-17
EP0975806B1 (de) 2006-06-21
US9556466B2 (en) 2017-01-31

Similar Documents

Publication Publication Date Title
DE69835008D1 (de) Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
ATE360093T1 (de) Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE194015T1 (de) Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung
ES2186293T3 (es) Metodo para la amplificacion exponencial directa y secuenciacion de moleculas de dna y su aplicacion.
DE3750774D1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
DE60022541D1 (de) Polynukleotid amplifizierungsverfahren
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
BR9914159A (pt) Métodos de amplificação e sequenciamento de ácido nucléico
NO20012561D0 (no) Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
DE60227525D1 (de) Verfahren zur Herstellung von Nukleinsäuremolekülen
ATE484515T1 (de) Dna-polymerase-mischungen und mutante dna- polymerasen
CA2127188A1 (en) Reagents and Methods for Coupled High Temperature Reverse Transcription and Polymerase Chain Reactions
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
DE60331132D1 (de) Verfahren zur Quantifizierung von Nukleinsäuren
DE59912683D1 (de) Verfahren und Zusammensetzungen zur Isolierung und Reinigung von Nukleinsäuren
ATE367452T1 (de) Methode zur reversen transkription von im getrockneten zustand isolierter rna

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: GRAF VON STOSCH PATENTANWALTSGESELLSCHAFT MBH, 805

8327 Change in the person/name/address of the patent owner

Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US