BR9914159A - Métodos de amplificação e sequenciamento de ácido nucléico - Google Patents

Métodos de amplificação e sequenciamento de ácido nucléico

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Publication number
BR9914159A
BR9914159A BR9914159-0A BR9914159A BR9914159A BR 9914159 A BR9914159 A BR 9914159A BR 9914159 A BR9914159 A BR 9914159A BR 9914159 A BR9914159 A BR 9914159A
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Brazil
Prior art keywords
nucleic acid
solid support
sequence
methods
oligonucleotide
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BR9914159-0A
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English (en)
Inventor
Pascal Mayer
Celine Adessi
Jean-Jacques Mermod
Eric Kawashima
Gerardo Turcatti
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Applied Research Systems
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Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of BR9914159A publication Critical patent/BR9914159A/pt

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Patente de Invenção: <B>"MéTODOS DE AMPLIFICAçãO E SEQuENCIAMENTO DE áCIDO NUCLéICO"<D>. Métodos para amplificação e sequenciamento de pelo menos um ácido nucléico compreendendo as seguintes etapas: (1) formar pelo menos um padrão de ácido nucléico compreendendo o(s) ácido(s) nucléico(s) a serem amplificados, onde o(s) referido(s) ácido(s) nucléico(s) contêm na extremidade 5' uma seq³ência Y de oligonucleotídeo e na extremidade 3' uma seq³ência Z de oligonucleotídeo e, além disso, o(s) ácidos nucléicos transportam na extremidade 5' um recurso para ligar o(s) ácido(s) nucléico(s) a um susporte sólido; (2) misturar os referidos padrões de ácido nucléico com um ou mais iniciadores X de colónia, que podem hibridizar para a seq³ência Z de oligonucleotídeo e transportam na extremidade 5' um recurso para ligação dos iniciadores de colónia a um suporte sólido, na presença de um suporte sólido a fim de que as extremidades 5' de igualmente o padrão de ácido nucléico e os iniciadores de colónia liguem-se ao suporte sólido; (3) realizar uma ou mais reações de amplificação de ácido nucléico nos padrões de ligação, a fim de que as colónias de ácido nucléico sejam geradas e, opcionalmente, realizar pelo menos uma etapa de determinação de seq³ência de uma ou mais colónias de ácido nucléico geradas. Suportes sólidos, kits e mecanismo para uso nestes métodos.
BR9914159-0A 1998-09-30 1999-09-30 Métodos de amplificação e sequenciamento de ácido nucléico BR9914159A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98307985 1998-09-30
PCT/GB1999/003248 WO2000018957A1 (en) 1998-09-30 1999-09-30 Methods of nucleic acid amplification and sequencing

Publications (1)

Publication Number Publication Date
BR9914159A true BR9914159A (pt) 2001-06-26

Family

ID=8235083

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9914159-0A BR9914159A (pt) 1998-09-30 1999-09-30 Métodos de amplificação e sequenciamento de ácido nucléico

Country Status (25)

Country Link
US (4) US7115400B1 (pt)
EP (2) EP1634963B1 (pt)
JP (1) JP2002525125A (pt)
KR (1) KR20010085860A (pt)
CN (1) CN1325458A (pt)
AR (1) AR021833A1 (pt)
AT (1) ATE311474T1 (pt)
AU (1) AU771073B2 (pt)
BG (1) BG105363A (pt)
BR (1) BR9914159A (pt)
CA (1) CA2344575A1 (pt)
CZ (1) CZ20011183A3 (pt)
DE (1) DE69928683T2 (pt)
EA (1) EA004271B1 (pt)
EE (1) EE200100195A (pt)
HK (1) HK1042525A1 (pt)
HU (1) HUP0105052A3 (pt)
IL (1) IL142178A0 (pt)
NO (1) NO20011303L (pt)
NZ (1) NZ510599A (pt)
PL (1) PL347125A1 (pt)
SK (1) SK4292001A3 (pt)
TR (1) TR200100911T2 (pt)
WO (1) WO2000018957A1 (pt)
ZA (1) ZA200102420B (pt)

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