ATE443136T1 - Methode für dna synthese - Google Patents

Methode für dna synthese

Info

Publication number
ATE443136T1
ATE443136T1 AT07104945T AT07104945T ATE443136T1 AT E443136 T1 ATE443136 T1 AT E443136T1 AT 07104945 T AT07104945 T AT 07104945T AT 07104945 T AT07104945 T AT 07104945T AT E443136 T1 ATE443136 T1 AT E443136T1
Authority
AT
Austria
Prior art keywords
dna synthesis
dna
synthesis reaction
enhancer
reaction
Prior art date
Application number
AT07104945T
Other languages
English (en)
Inventor
Kiyozo Asada
Yoshimi Sato
Takashi Uemori
Tomoko Fujita
Kazue Miyake
Osamu Takeda
Hiroyuki Mukai
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE443136T1 publication Critical patent/ATE443136T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AT07104945T 1998-04-23 1999-04-21 Methode für dna synthese ATE443136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11400598 1998-04-23
JP31524398 1998-11-06

Publications (1)

Publication Number Publication Date
ATE443136T1 true ATE443136T1 (de) 2009-10-15

Family

ID=26452869

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07104945T ATE443136T1 (de) 1998-04-23 1999-04-21 Methode für dna synthese

Country Status (10)

Country Link
US (2) US7521178B1 (de)
EP (3) EP1072678A4 (de)
JP (2) JP3748378B2 (de)
KR (1) KR100519895B1 (de)
CN (2) CN1637146A (de)
AT (1) ATE443136T1 (de)
AU (1) AU3534199A (de)
CA (1) CA2326725A1 (de)
DE (1) DE69941441D1 (de)
WO (1) WO1999054455A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3724321B2 (ja) * 2000-03-21 2005-12-07 株式会社島津製作所 核酸合成法
TW200525026A (en) 2003-12-25 2005-08-01 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
JP2008151771A (ja) 2006-11-22 2008-07-03 Fujifilm Corp マイクロ流体チップ
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
JP4450867B2 (ja) 2007-10-25 2010-04-14 独立行政法人理化学研究所 等温増幅方法およびそれに用いる等温増幅用キット
US20110059869A1 (en) * 2008-02-29 2011-03-10 Riken Method for Increasing Enzymatic Reactivity
EP2371951A4 (de) 2008-11-27 2012-07-18 Riken NEUARTIGES MutS-PROTEIN UND SEINE VERWENDUNG ZUR BESTIMMUNG VON MUTATIONEN
EP2382321A4 (de) * 2009-01-08 2012-12-19 Bio Rad Laboratories Verfahren und zusammensetzungen zur verbesserung der wirksamkeit bei einer nukleinsäureverstärkungsreaktionen
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
ES3005233T3 (en) 2010-10-01 2025-03-14 Modernatx Inc Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
KR101609224B1 (ko) 2011-01-24 2016-04-05 다카라 바이오 가부시키가이샤 핵산 변형 방법
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
EP2746395B1 (de) 2011-09-08 2017-11-29 Kabushiki Kaisha DNAFORM Primer-set, verfahren zur amplifizierung einer nukleinsäuresequenz damit und verfahren zur erkennung von mutierter nukleinsäure damit
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2763701B1 (de) 2011-10-03 2018-12-19 Moderna Therapeutics, Inc. Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon
JP2015501844A (ja) 2011-12-16 2015-01-19 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 修飾ヌクレオシド、ヌクレオチドおよび核酸組成物
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
WO2013151663A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of membrane proteins
EP2895610B1 (de) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Zusammensetzungen und systeme zur induzierung von krankheitsresistenz bei pflanzen und verfahren zu ihrer verwendung
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
US20160194625A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Chimeric polynucleotides
KR20160067219A (ko) 2013-10-03 2016-06-13 모더나 세라퓨틱스, 인코포레이티드 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드
JP2017501730A (ja) 2013-12-31 2017-01-19 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｄｎａメチル化の状態を通してゲノム機能のエピジェネティックな調節を評価する方法ならびにそのためのシステムおよびキット
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
EP3169693B1 (de) 2014-07-16 2022-03-09 ModernaTX, Inc. Chimäre polynukleotide
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
KR101785074B1 (ko) 2015-01-28 2017-10-12 고려대학교 산학협력단 Dna 합성용 마이크로 칩 및 이를 이용한 dna 합성방법
WO2017112943A1 (en) 2015-12-23 2017-06-29 Modernatx, Inc. Methods of using ox40 ligand encoding polynucleotides
WO2017120612A1 (en) 2016-01-10 2017-07-13 Modernatx, Inc. Therapeutic mrnas encoding anti ctla-4 antibodies
WO2017201325A1 (en) 2016-05-18 2017-11-23 Modernatx, Inc. Combinations of mrnas encoding immune modulating polypeptides and uses thereof
DK3458083T5 (da) 2016-05-18 2024-10-21 Modernatx Inc Polynukleotider, der koder for interleukin-12 (il12), og anvendelser heraf
CN106916909B (zh) * 2017-05-09 2018-09-11 广州海力特生物科技有限公司 一种HBV pgRNA的实时荧光定量RT-PCR检测引物组、探针组、试剂盒及方法
US20200131498A1 (en) 2017-06-14 2020-04-30 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
WO2020185312A2 (en) 2019-01-25 2020-09-17 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
EP4073246A4 (de) 2019-12-12 2023-12-06 President And Fellows Of Harvard College Zusammensetzungen und verfahren zur lichtgerichteten biomolekularen barcodierung
EP4139456A4 (de) 2020-04-22 2024-05-22 President And Fellows Of Harvard College Isothermische verfahren, zusammensetzungen, kits und systeme zum nachweis von nukleinsäuren
JP2023545038A (ja) 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム
WO2025106313A1 (en) 2023-11-14 2025-05-22 Eli Lilly And Company Fc RECEPTOR BASED ASSAYS FOR ANALYZING IgG ANTIBODIES
EP4729632A1 (de) 2024-10-18 2026-04-22 AskBio Inc. Verfahren zur synthese von dna

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
US5225556A (en) * 1985-01-18 1993-07-06 The Trustees Of Columbia University In The City Of New York Chemical probes for left-handed DNA and for A-DNA; chiral metal complexes as Z-specific antitumor agents and as double strand cleavers
DE3601136A1 (de) * 1986-01-16 1987-07-23 Max Planck Gesellschaft Hemmstoffe der reversen transkriptase fuer prophylaxe und therapie von retrovirus-infektionen in saeugetieren
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
US5554498A (en) * 1991-08-30 1996-09-10 Syngenix Limited Nucleic acid amplification using scandium and lanthanum ions
US5418162A (en) * 1991-10-22 1995-05-23 Duke University Serotonin transporter CDNA
WO1993023574A1 (en) * 1992-05-14 1993-11-25 Kozal Michael J Polymerase chain reaction assays for monitoring antiviral therapy
DE69332852T2 (de) * 1992-09-11 2003-12-04 F. Hoffmann-La Roche Ag, Basel Nachweis von Nukleinsäuren im Blut
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US6087097A (en) * 1994-05-12 2000-07-11 Mayo Foundation For Medical Education And Research PCR detection of Borrelia burgdorferi
EP0745687A1 (de) 1995-04-08 1996-12-04 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5789206A (en) * 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
EP0870832A4 (de) 1995-12-27 2001-08-22 Takara Shuzo Co Neue dna polymerase
KR100555845B1 (ko) * 1996-01-26 2006-11-23 다카라 홀딩스 인크. 항암제
US6156504A (en) * 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US5917031A (en) * 1996-04-19 1999-06-29 Taiko Pharmaceutical Co., Ltd. Method of synthesizing polydeoxyribonucleotides
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
JP3505185B2 (ja) * 1996-06-12 2004-03-08 タカラバイオ株式会社 食品又は飲料
EP0822256B1 (de) 1996-07-29 2003-09-24 Toyo Boseki Kabushiki Kaisha Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
JP3463780B2 (ja) 1996-07-30 2003-11-05 東洋紡績株式会社 核酸増幅用ｄｎａポリメラーゼ組成物
ES2355739T3 (es) * 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
EP1111044A4 (de) * 1998-09-08 2003-06-04 Takara Shuzo Co Methode zur dna-synthese
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
JP2001029078A (ja) * 1999-07-16 2001-02-06 Shimadzu Corp Ｒｎａ増幅法
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases

Also Published As

Publication number Publication date
EP1816197A2 (de) 2007-08-08
JP3748378B2 (ja) 2006-02-22
EP1072678A4 (de) 2006-09-27
AU3534199A (en) 1999-11-08
EP2116600B1 (de) 2013-09-18
JP4214142B2 (ja) 2009-01-28
JP2006061164A (ja) 2006-03-09
CN1177931C (zh) 2004-12-01
KR100519895B1 (ko) 2005-10-13
KR20010042958A (ko) 2001-05-25
US20090098613A1 (en) 2009-04-16
CA2326725A1 (en) 1999-10-28
US7521178B1 (en) 2009-04-21
DE69941441D1 (de) 2009-10-29
EP1072678A1 (de) 2001-01-31
WO1999054455A1 (fr) 1999-10-28
EP1816197A3 (de) 2007-11-28
US8367328B2 (en) 2013-02-05
CN1637146A (zh) 2005-07-13
EP2116600A3 (de) 2010-01-13
CN1306570A (zh) 2001-08-01
EP1816197B1 (de) 2009-09-16
EP2116600A2 (de) 2009-11-11

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