ATE443136T1 - Methode für dna synthese - Google Patents

Methode für dna synthese

Info

Publication number
ATE443136T1
ATE443136T1 AT07104945T AT07104945T ATE443136T1 AT E443136 T1 ATE443136 T1 AT E443136T1 AT 07104945 T AT07104945 T AT 07104945T AT 07104945 T AT07104945 T AT 07104945T AT E443136 T1 ATE443136 T1 AT E443136T1
Authority
AT
Austria
Prior art keywords
dna synthesis
dna
synthesis reaction
enhancer
reaction
Prior art date
Application number
AT07104945T
Other languages
English (en)
Inventor
Kiyozo Asada
Yoshimi Sato
Takashi Uemori
Tomoko Fujita
Kazue Miyake
Osamu Takeda
Hiroyuki Mukai
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE443136T1 publication Critical patent/ATE443136T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AT07104945T 1998-04-23 1999-04-21 Methode für dna synthese ATE443136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11400598 1998-04-23
JP31524398 1998-11-06

Publications (1)

Publication Number Publication Date
ATE443136T1 true ATE443136T1 (de) 2009-10-15

Family

ID=26452869

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07104945T ATE443136T1 (de) 1998-04-23 1999-04-21 Methode für dna synthese

Country Status (10)

Country Link
US (2) US7521178B1 (de)
EP (3) EP1072678A4 (de)
JP (2) JP3748378B2 (de)
KR (1) KR100519895B1 (de)
CN (2) CN1637146A (de)
AT (1) ATE443136T1 (de)
AU (1) AU3534199A (de)
CA (1) CA2326725A1 (de)
DE (1) DE69941441D1 (de)
WO (1) WO1999054455A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3724321B2 (ja) * 2000-03-21 2005-12-07 株式会社島津製作所 核酸合成法
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
JP2008151771A (ja) 2006-11-22 2008-07-03 Fujifilm Corp マイクロ流体チップ
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
EP2210941A4 (de) 2007-10-25 2010-11-03 Riken Isothermes amplifikationsverfahren und dabei verwendete dna-polymerase
EP2256188B1 (de) * 2008-02-29 2014-06-18 Riken Verfahren zur erhöhung der enzymatischen reaktivität
US20110236900A1 (en) 2008-11-27 2011-09-29 Riken Novel muts protein and method for determing mutation using the same
SG10201500705YA (en) * 2009-01-08 2015-04-29 Bio Rad Laboratories Methods and compositions for improving efficiency of nucleic acids amplification reactions
EP3502272A1 (de) * 2010-06-21 2019-06-26 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
US8822663B2 (en) 2010-08-06 2014-09-02 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CA2821992A1 (en) 2010-10-01 2012-04-05 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
WO2012102208A1 (ja) 2011-01-24 2012-08-02 タカラバイオ株式会社 核酸修飾方法
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
HRP20220250T1 (hr) 2011-10-03 2022-04-29 Modernatx, Inc. Modificirani nukleozidi, nukleotidi i nukleinske kiseline, te njihove uporabe
SI2791160T1 (sl) 2011-12-16 2022-07-29 Modernatx, Inc. Sestave modificirane MRNA
AU2013243952A1 (en) 2012-04-02 2014-10-30 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
WO2014042923A1 (en) 2012-09-13 2014-03-20 Indiana University Research & Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
SMT202200337T1 (it) 2012-11-26 2022-09-14 Modernatx Inc Rna modificato al livello del terminale
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
AU2014315287A1 (en) 2013-09-03 2015-03-12 Moderna Therapeutics, Inc. Chimeric polynucleotides
US10323076B2 (en) 2013-10-03 2019-06-18 Modernatx, Inc. Polynucleotides encoding low density lipoprotein receptor
US20150259743A1 (en) 2013-12-31 2015-09-17 Roche Nimblegen, Inc. Methods of assessing epigenetic regulation of genome function via dna methylation status and systems and kits therefor
EP3094753B1 (de) 2014-01-15 2019-10-16 Abbott Laboratories Dna für sequenzumwandlung und nachweisverfahren
WO2016011226A1 (en) 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Chimeric polynucleotides
CN108064312B (zh) 2014-12-24 2022-04-08 雅培实验室 基于具有靶扩增的信号放大dna级联反应的检测方法
KR101785074B1 (ko) 2015-01-28 2017-10-12 고려대학교 산학협력단 Dna 합성용 마이크로 칩 및 이를 이용한 dna 합성방법
PL3394093T3 (pl) 2015-12-23 2022-05-16 Modernatx, Inc. Metody stosowania polinukleotydów kodujących ligand ox40
EP3400023A1 (de) 2016-01-10 2018-11-14 ModernaTX, Inc. Therapeutische mrnas zur codierung von anti-ctla-4-antikörpern
DK3458083T5 (da) 2016-05-18 2024-10-21 Modernatx Inc Polynukleotider, der koder for interleukin-12 (il12), og anvendelser heraf
LT3458474T (lt) 2016-05-18 2022-10-10 Modernatx, Inc. Imunitetą moduliuojančius polipeptidus koduojančių mrnr deriniai ir jų naudojimas
CN106916909B (zh) * 2017-05-09 2018-09-11 广州海力特生物科技有限公司 一种HBV pgRNA的实时荧光定量RT-PCR检测引物组、探针组、试剂盒及方法
US20200131498A1 (en) 2017-06-14 2020-04-30 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
US20220056498A1 (en) 2019-01-25 2022-02-24 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
US20230029257A1 (en) 2019-12-12 2023-01-26 President And Fellows Of Harvard College Compositions and methods for light-directed biomolecular barcoding
WO2021216728A1 (en) 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
JP2023545038A (ja) 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム
WO2025106313A1 (en) 2023-11-14 2025-05-22 Eli Lilly And Company Fc RECEPTOR BASED ASSAYS FOR ANALYZING IgG ANTIBODIES

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
US5225556A (en) * 1985-01-18 1993-07-06 The Trustees Of Columbia University In The City Of New York Chemical probes for left-handed DNA and for A-DNA; chiral metal complexes as Z-specific antitumor agents and as double strand cleavers
DE3601136A1 (de) * 1986-01-16 1987-07-23 Max Planck Gesellschaft Hemmstoffe der reversen transkriptase fuer prophylaxe und therapie von retrovirus-infektionen in saeugetieren
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
WO1993005174A1 (en) * 1991-08-30 1993-03-18 Syngenix Limited Nucleic acid polymerase amplification
US5418162A (en) * 1991-10-22 1995-05-23 Duke University Serotonin transporter CDNA
WO1993023574A1 (en) * 1992-05-14 1993-11-25 Kozal Michael J Polymerase chain reaction assays for monitoring antiviral therapy
DK0590327T3 (da) * 1992-09-11 2003-07-28 Hoffmann La Roche Påvisning af nukleinsyrer i blod
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US6087097A (en) * 1994-05-12 2000-07-11 Mayo Foundation For Medical Education And Research PCR detection of Borrelia burgdorferi
EP0745687A1 (de) 1995-04-08 1996-12-04 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5789206A (en) * 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
CA2241170A1 (en) 1995-12-27 1997-07-10 Takara Shuzo Co., Ltd. Novel dna polymerase
CN1150003C (zh) * 1996-01-26 2004-05-19 宝酒造株式会社 细胞凋亡诱导物
AU2324997A (en) * 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
US5917031A (en) * 1996-04-19 1999-06-29 Taiko Pharmaceutical Co., Ltd. Method of synthesizing polydeoxyribonucleotides
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
CA2253573C (en) * 1996-06-12 2005-12-06 Takara Shuzo Co., Ltd. Fucoidan-containing foods or beverages
JP3463780B2 (ja) 1996-07-30 2003-11-05 東洋紡績株式会社 核酸増幅用dnaポリメラーゼ組成物
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
WO1998044161A1 (en) * 1997-04-03 1998-10-08 Life Technologies, Inc. Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
EP1111044A4 (de) * 1998-09-08 2003-06-04 Takara Shuzo Co Methode zur dna-synthese
EP0989192A3 (de) 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
JP2001029078A (ja) 1999-07-16 2001-02-06 Shimadzu Corp Rna増幅法
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases

Also Published As

Publication number Publication date
CN1306570A (zh) 2001-08-01
EP1816197A3 (de) 2007-11-28
EP2116600B1 (de) 2013-09-18
JP3748378B2 (ja) 2006-02-22
EP1816197A2 (de) 2007-08-08
CA2326725A1 (en) 1999-10-28
KR20010042958A (ko) 2001-05-25
CN1177931C (zh) 2004-12-01
KR100519895B1 (ko) 2005-10-13
AU3534199A (en) 1999-11-08
DE69941441D1 (de) 2009-10-29
WO1999054455A1 (fr) 1999-10-28
US20090098613A1 (en) 2009-04-16
JP4214142B2 (ja) 2009-01-28
CN1637146A (zh) 2005-07-13
US7521178B1 (en) 2009-04-21
EP1072678A4 (de) 2006-09-27
EP2116600A3 (de) 2010-01-13
US8367328B2 (en) 2013-02-05
EP1072678A1 (de) 2001-01-31
EP2116600A2 (de) 2009-11-11
JP2006061164A (ja) 2006-03-09
EP1816197B1 (de) 2009-09-16

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