ATE443136T1 - Methode für dna synthese - Google Patents
Methode für dna syntheseInfo
- Publication number
- ATE443136T1 ATE443136T1 AT07104945T AT07104945T ATE443136T1 AT E443136 T1 ATE443136 T1 AT E443136T1 AT 07104945 T AT07104945 T AT 07104945T AT 07104945 T AT07104945 T AT 07104945T AT E443136 T1 ATE443136 T1 AT E443136T1
- Authority
- AT
- Austria
- Prior art keywords
- dna synthesis
- dna
- synthesis reaction
- enhancer
- reaction
- Prior art date
Links
- 230000006820 DNA synthesis Effects 0.000 title abstract 13
- 238000000034 method Methods 0.000 title 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 5
- 238000003786 synthesis reaction Methods 0.000 abstract 5
- 239000003623 enhancer Substances 0.000 abstract 4
- 108010010677 Phosphodiesterase I Proteins 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 3
- 238000000338 in vitro Methods 0.000 abstract 2
- 238000001308 synthesis method Methods 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 125000002091 cationic group Chemical group 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11400598 | 1998-04-23 | ||
JP31524398 | 1998-11-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE443136T1 true ATE443136T1 (de) | 2009-10-15 |
Family
ID=26452869
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07104945T ATE443136T1 (de) | 1998-04-23 | 1999-04-21 | Methode für dna synthese |
Country Status (10)
Country | Link |
---|---|
US (2) | US7521178B1 (de) |
EP (3) | EP2116600B1 (de) |
JP (2) | JP3748378B2 (de) |
KR (1) | KR100519895B1 (de) |
CN (2) | CN1177931C (de) |
AT (1) | ATE443136T1 (de) |
AU (1) | AU3534199A (de) |
CA (1) | CA2326725A1 (de) |
DE (1) | DE69941441D1 (de) |
WO (1) | WO1999054455A1 (de) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3724321B2 (ja) * | 2000-03-21 | 2005-12-07 | 株式会社島津製作所 | 核酸合成法 |
TW201037080A (en) | 2003-12-25 | 2010-10-16 | Danform Kk | Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same |
US7462475B2 (en) * | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
JP2008151771A (ja) | 2006-11-22 | 2008-07-03 | Fujifilm Corp | マイクロ流体チップ |
CA2622649C (en) | 2007-03-09 | 2018-04-24 | Riken | Nucleic acid amplification method using primer exhibiting exciton effect |
WO2009054510A1 (ja) | 2007-10-25 | 2009-04-30 | Riken | 等温増幅方法およびそれに用いるdnaポリメラーゼ |
EP2256188B1 (de) * | 2008-02-29 | 2014-06-18 | Riken | Verfahren zur erhöhung der enzymatischen reaktivität |
WO2010061922A1 (ja) | 2008-11-27 | 2010-06-03 | 独立行政法人理化学研究所 | 新規MutSタンパク質およびそれを用いた変異の判定方法 |
CN102272319B (zh) * | 2009-01-08 | 2014-08-27 | 伯乐实验室公司 | 用于提高核酸扩增反应效率的方法和组合物 |
EP3502272A1 (de) * | 2010-06-21 | 2019-06-26 | Life Technologies Corporation | Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren |
EP3578205A1 (de) | 2010-08-06 | 2019-12-11 | ModernaTX, Inc. | Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung |
CN104531812A (zh) | 2010-10-01 | 2015-04-22 | 现代治疗公司 | 设计核酸及其使用方法 |
CN103443294B (zh) * | 2011-01-24 | 2015-09-30 | 宝生物工程株式会社 | 用于修饰核酸的方法 |
JP2014511687A (ja) | 2011-03-31 | 2014-05-19 | モデルナ セラピューティクス インコーポレイテッド | 工学操作された核酸の送達および製剤 |
EP2746395B1 (de) | 2011-09-08 | 2017-11-29 | Kabushiki Kaisha DNAFORM | Primer-set, verfahren zur amplifizierung einer nukleinsäuresequenz damit und verfahren zur erkennung von mutierter nukleinsäure damit |
US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
ES2911677T3 (es) | 2011-10-03 | 2022-05-20 | Modernatx Inc | Nucleósidos, nucleótidos y ácidos nucleicos modificados, y sus usos |
RS63244B1 (sr) | 2011-12-16 | 2022-06-30 | Modernatx Inc | Kompozicije modifikovane mrna |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
US20140010861A1 (en) | 2012-04-02 | 2014-01-09 | modeRNA Therapeutics | Modified polynucleotides for the production of proteins associated with human disease |
US9878056B2 (en) | 2012-04-02 | 2018-01-30 | Modernatx, Inc. | Modified polynucleotides for the production of cosmetic proteins and peptides |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
BR112015005389B1 (pt) | 2012-09-13 | 2022-10-18 | Indiana University Research And Technology Corporation | Molécula de ácido nucleico recombinante, proteína de substrato modificada de uma protease específica de patógeno de planta expressa através do patógeno de planta, vetor, e método para proteger uma planta da infecção por um patógeno de planta que secreta pelo menos uma protease específica |
LT2922554T (lt) | 2012-11-26 | 2022-06-27 | Modernatx, Inc. | Terminaliai modifikuota rnr |
US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
JP2016530294A (ja) | 2013-09-03 | 2016-09-29 | モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. | キメラポリヌクレオチド |
KR20160067219A (ko) | 2013-10-03 | 2016-06-13 | 모더나 세라퓨틱스, 인코포레이티드 | 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드 |
JP2017501730A (ja) | 2013-12-31 | 2017-01-19 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | Dnaメチル化の状態を通してゲノム機能のエピジェネティックな調節を評価する方法ならびにそのためのシステムおよびキット |
US10036077B2 (en) | 2014-01-15 | 2018-07-31 | Abbott Laboratories | Covered sequence conversion DNA and detection methods |
CA2955250A1 (en) | 2014-07-16 | 2016-01-21 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
WO2016103234A1 (en) | 2014-12-24 | 2016-06-30 | Abbott Japan Co., Ltd. | Detection method based on signal amplifier dna cascade reactions with target extension |
KR101785074B1 (ko) | 2015-01-28 | 2017-10-12 | 고려대학교 산학협력단 | Dna 합성용 마이크로 칩 및 이를 이용한 dna 합성방법 |
HRP20220525T1 (hr) | 2015-12-23 | 2022-05-27 | Modernatx, Inc. | Postupci uporabe polinukleotida koji kodiraju ligand ox40 |
EP3400023A1 (de) | 2016-01-10 | 2018-11-14 | ModernaTX, Inc. | Therapeutische mrnas zur codierung von anti-ctla-4-antikörpern |
LT3458083T (lt) | 2016-05-18 | 2023-02-10 | Modernatx, Inc. | Polinukleotidai, koduojantys interleukiną-12 (il12), ir jų naudojimas |
JP7194594B2 (ja) | 2016-05-18 | 2022-12-22 | モデルナティエックス インコーポレイテッド | 免疫調節ポリペプチドをコードするmRNAの組み合わせ及びその使用 |
CN106916909B (zh) * | 2017-05-09 | 2018-09-11 | 广州海力特生物科技有限公司 | 一种HBV pgRNA的实时荧光定量RT-PCR检测引物组、探针组、试剂盒及方法 |
WO2018231990A2 (en) | 2017-06-14 | 2018-12-20 | Modernatx, Inc. | Polynucleotides encoding methylmalonyl-coa mutase |
BR112020016306A2 (pt) | 2018-02-12 | 2020-12-15 | Curators Of The University Of Missouri | Gene (saur) suprarregulado pequeno de auxina para o melhoramento da arquitetura do sistema radicular da planta, tolerância ao encharcamento, resistência à seca, e rendimento |
JP2022518489A (ja) | 2019-01-25 | 2022-03-15 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 核酸を合成するための組成物および方法 |
WO2021119402A1 (en) | 2019-12-12 | 2021-06-17 | President And Fellows Of Harvard College | Compositions and methods for light-directed biomolecular barcoding |
AU2021261343A1 (en) | 2020-04-22 | 2022-11-03 | President And Fellows Of Harvard College | Isothermal methods, compositions, kits, and systems for detecting nucleic acids |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4072574A (en) * | 1976-07-30 | 1978-02-07 | The Institute For Cancer Research | System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents |
US5225556A (en) * | 1985-01-18 | 1993-07-06 | The Trustees Of Columbia University In The City Of New York | Chemical probes for left-handed DNA and for A-DNA; chiral metal complexes as Z-specific antitumor agents and as double strand cleavers |
DE3601136A1 (de) * | 1986-01-16 | 1987-07-23 | Max Planck Gesellschaft | Hemmstoffe der reversen transkriptase fuer prophylaxe und therapie von retrovirus-infektionen in saeugetieren |
CA2065719A1 (en) | 1991-04-30 | 1992-10-31 | John B. Findlay | Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle |
DE69231178T2 (de) * | 1991-08-30 | 2000-11-23 | Syngenix Ltd | Nukleinsäure-amplifikation durch polymerase-reaktion |
US5418162A (en) * | 1991-10-22 | 1995-05-23 | Duke University | Serotonin transporter CDNA |
WO1993023574A1 (en) * | 1992-05-14 | 1993-11-25 | Kozal Michael J | Polymerase chain reaction assays for monitoring antiviral therapy |
DK0590327T3 (da) * | 1992-09-11 | 2003-07-28 | Hoffmann La Roche | Påvisning af nukleinsyrer i blod |
RU2048522C1 (ru) * | 1992-10-14 | 1995-11-20 | Институт белка РАН | Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления |
US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
US6087097A (en) * | 1994-05-12 | 2000-07-11 | Mayo Foundation For Medical Education And Research | PCR detection of Borrelia burgdorferi |
EP0745687A1 (de) | 1995-04-08 | 1996-12-04 | Roche Diagnostics GmbH | Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA |
US5789206A (en) * | 1995-07-07 | 1998-08-04 | Myriad Genetics, Inc. | Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes |
US5795722A (en) * | 1997-03-18 | 1998-08-18 | Visible Genetics Inc. | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
US6395526B1 (en) | 1995-12-27 | 2002-05-28 | Takara Shuzo Co., Ltd. | DNA polymerase |
CA2243543A1 (en) * | 1996-01-26 | 1997-07-31 | Research Institute For Glycotechnology | Apoptosis inducers |
CA2248981C (en) * | 1996-03-15 | 2009-11-24 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
US5917031A (en) | 1996-04-19 | 1999-06-29 | Taiko Pharmaceutical Co., Ltd. | Method of synthesizing polydeoxyribonucleotides |
US5928906A (en) * | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
CA2253573C (en) | 1996-06-12 | 2005-12-06 | Takara Shuzo Co., Ltd. | Fucoidan-containing foods or beverages |
DE69725076T2 (de) | 1996-07-29 | 2004-04-15 | Toyo Boseki K.K. | Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren |
JP3463780B2 (ja) | 1996-07-30 | 2003-11-05 | 東洋紡績株式会社 | 核酸増幅用dnaポリメラーゼ組成物 |
EP1705253B1 (de) * | 1997-04-03 | 2010-11-03 | Life Technologies Corporation | Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR) |
US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
US6348336B1 (en) * | 1997-07-01 | 2002-02-19 | Alpha Therapeutic Corporation | Process for purification of PCR test samples |
EP1111044A4 (de) * | 1998-09-08 | 2003-06-04 | Takara Shuzo Co | Methode zur dna-synthese |
EP0989192A3 (de) * | 1998-09-21 | 2004-02-25 | Shimadzu Corporation | Verfahren zur Nucleinsäuresynthese |
JP2001029078A (ja) | 1999-07-16 | 2001-02-06 | Shimadzu Corp | Rna増幅法 |
GB0001450D0 (en) * | 2000-01-21 | 2000-03-08 | Genpoint As | Cell isolation method |
DE10006662A1 (de) * | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
US6667165B2 (en) * | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
-
1999
- 1999-04-21 US US09/673,884 patent/US7521178B1/en not_active Expired - Fee Related
- 1999-04-21 EP EP09167571.0A patent/EP2116600B1/de not_active Expired - Lifetime
- 1999-04-21 DE DE69941441T patent/DE69941441D1/de not_active Expired - Lifetime
- 1999-04-21 CN CNB998077097A patent/CN1177931C/zh not_active Expired - Lifetime
- 1999-04-21 EP EP99917081A patent/EP1072678A4/de not_active Withdrawn
- 1999-04-21 CN CNA2004100825674A patent/CN1637146A/zh active Pending
- 1999-04-21 AT AT07104945T patent/ATE443136T1/de not_active IP Right Cessation
- 1999-04-21 EP EP07104945A patent/EP1816197B1/de not_active Expired - Lifetime
- 1999-04-21 KR KR10-2000-7011784A patent/KR100519895B1/ko not_active IP Right Cessation
- 1999-04-21 WO PCT/JP1999/002121 patent/WO1999054455A1/ja not_active Application Discontinuation
- 1999-04-21 CA CA002326725A patent/CA2326725A1/en not_active Abandoned
- 1999-04-21 JP JP2000544787A patent/JP3748378B2/ja not_active Expired - Lifetime
- 1999-04-21 AU AU35341/99A patent/AU3534199A/en not_active Abandoned
-
2005
- 2005-10-14 JP JP2005300626A patent/JP4214142B2/ja not_active Expired - Fee Related
-
2008
- 2008-10-15 US US12/285,866 patent/US8367328B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20090098613A1 (en) | 2009-04-16 |
KR100519895B1 (ko) | 2005-10-13 |
CA2326725A1 (en) | 1999-10-28 |
EP1816197A2 (de) | 2007-08-08 |
US8367328B2 (en) | 2013-02-05 |
EP2116600A2 (de) | 2009-11-11 |
EP2116600B1 (de) | 2013-09-18 |
CN1177931C (zh) | 2004-12-01 |
CN1637146A (zh) | 2005-07-13 |
JP4214142B2 (ja) | 2009-01-28 |
EP1072678A1 (de) | 2001-01-31 |
EP1072678A4 (de) | 2006-09-27 |
KR20010042958A (ko) | 2001-05-25 |
US7521178B1 (en) | 2009-04-21 |
DE69941441D1 (de) | 2009-10-29 |
JP3748378B2 (ja) | 2006-02-22 |
WO1999054455A1 (en) | 1999-10-28 |
AU3534199A (en) | 1999-11-08 |
JP2006061164A (ja) | 2006-03-09 |
EP1816197B1 (de) | 2009-09-16 |
EP2116600A3 (de) | 2010-01-13 |
EP1816197A3 (de) | 2007-11-28 |
CN1306570A (zh) | 2001-08-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE443136T1 (de) | Methode für dna synthese | |
EP2272952B8 (de) | Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität | |
CA2310854A1 (en) | Isolation and identification of novel polymerases | |
DE69627698D1 (de) | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren | |
DE69637256D1 (de) | Synthese von Methoxynukleoside und enzymatische Nukleisäure Moleküle | |
PT669401E (pt) | Amplificacao de sequencias compridas de acidos nucleicos | |
DE69431669D1 (de) | Enzymatische nukleiksaüre die nicht-nukleotide enthaltet | |
ATE374833T1 (de) | Verfahren zur synthese von nukleinsäuren | |
ATE244259T1 (de) | Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen | |
WO2001038584A3 (en) | Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof | |
BR9407712A (pt) | Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos | |
DE69435232D1 (de) | DNS Polymerasen | |
FI960540A (fi) | Eksonukleaasin ja/tai glykosylaasin käyttö antipolymeraasivasta-aineenlisäaineina spesifisyyden lisäämiseksi polymeraasiketjureaktiossa | |
EP0822256A3 (de) | Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren | |
ATE317023T1 (de) | Methode zum nachweis von variationen oder polymorphismen | |
DE60231901D1 (de) | Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon | |
CA2295306A1 (en) | Dna polymerase-related factors | |
DE69734604D1 (de) | Synthetische polynukleotide | |
DK0625194T3 (da) | Forøgelse af ribozym-katalytisk aktivitet med et tilstødende facilitator oligonukleotid | |
ATE307199T1 (de) | Verfahren und zusammensetzungen zur isolierung und reinigung von nukleinsäuren | |
WO2003054145A3 (en) | Dinuclear copper-based compound and ligand for nucleic acid scission and anticancer treatment |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |