ATE443136T1 - Methode für dna synthese - Google Patents

Methode für dna synthese

Info

Publication number
ATE443136T1
ATE443136T1 AT07104945T AT07104945T ATE443136T1 AT E443136 T1 ATE443136 T1 AT E443136T1 AT 07104945 T AT07104945 T AT 07104945T AT 07104945 T AT07104945 T AT 07104945T AT E443136 T1 ATE443136 T1 AT E443136T1
Authority
AT
Austria
Prior art keywords
dna synthesis
dna
synthesis reaction
enhancer
reaction
Prior art date
Application number
AT07104945T
Other languages
English (en)
Inventor
Kiyozo Asada
Yoshimi Sato
Takashi Uemori
Tomoko Fujita
Kazue Miyake
Osamu Takeda
Hiroyuki Mukai
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE443136T1 publication Critical patent/ATE443136T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AT07104945T 1998-04-23 1999-04-21 Methode für dna synthese ATE443136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11400598 1998-04-23
JP31524398 1998-11-06

Publications (1)

Publication Number Publication Date
ATE443136T1 true ATE443136T1 (de) 2009-10-15

Family

ID=26452869

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07104945T ATE443136T1 (de) 1998-04-23 1999-04-21 Methode für dna synthese

Country Status (10)

Country Link
US (2) US7521178B1 (de)
EP (3) EP1816197B1 (de)
JP (2) JP3748378B2 (de)
KR (1) KR100519895B1 (de)
CN (2) CN1177931C (de)
AT (1) ATE443136T1 (de)
AU (1) AU3534199A (de)
CA (1) CA2326725A1 (de)
DE (1) DE69941441D1 (de)
WO (1) WO1999054455A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3724321B2 (ja) * 2000-03-21 2005-12-07 株式会社島津製作所 核酸合成法
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
JP2008151771A (ja) 2006-11-22 2008-07-03 Fujifilm Corp マイクロ流体チップ
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
US20100221787A1 (en) 2007-10-25 2010-09-02 Riken Isothermal amplification method and dna polymerase used in the same
EP2256188B1 (de) * 2008-02-29 2014-06-18 Riken Verfahren zur erhöhung der enzymatischen reaktivität
JPWO2010061922A1 (ja) 2008-11-27 2012-04-26 独立行政法人理化学研究所 新規ＭｕｔＳタンパク質およびそれを用いた変異の判定方法
EP2894226B1 (de) * 2009-01-08 2021-04-14 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzung zur verbesserung der wirksamkeit bei einer nukleinsäureverstärkungsreaktion
EP3502272A1 (de) * 2010-06-21 2019-06-26 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
US8822663B2 (en) 2010-08-06 2014-09-02 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
DK4108671T3 (da) 2010-10-01 2025-01-06 Modernatx Inc Modificerede nukleosider, nukleotider og nukleinsyrer samt anvendelser deraf
EP2669385B1 (de) 2011-01-24 2017-03-08 Takara Bio Inc. Verfahren für nukleinsäuremodifikation
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
JP5299981B1 (ja) 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
JP6113737B2 (ja) 2011-10-03 2017-04-12 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 修飾型のヌクレオシド、ヌクレオチドおよび核酸、ならびにそれらの使用方法
KR20140102759A (ko) 2011-12-16 2014-08-22 모더나 세라퓨틱스, 인코포레이티드 변형된 뉴클레오사이드, 뉴클레오타이드 및 핵산 조성물
EP2834259A4 (de) 2012-04-02 2016-08-24 Moderna Therapeutics Inc Modifizierte polynukleotide
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2895610B1 (de) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Zusammensetzungen und systeme zur induzierung von krankheitsresistenz bei pflanzen und verfahren zu ihrer verwendung
US9597380B2 (en) 2012-11-26 2017-03-21 Modernatx, Inc. Terminally modified RNA
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
AU2014315287A1 (en) 2013-09-03 2015-03-12 Moderna Therapeutics, Inc. Chimeric polynucleotides
EP3052521A1 (de) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynukleotide zur codierung von low-density-lipoprotein-rezeptor
CA2933514A1 (en) 2013-12-31 2015-07-09 F. Hoffmann-La Roche Ag Methods of assessing epigenetic regulation of genome function via dna methylation status and systems and kits therefor
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
EP3169693B1 (de) 2014-07-16 2022-03-09 ModernaTX, Inc. Chimäre polynukleotide
ES2791280T3 (es) 2014-12-24 2020-11-03 Abbott Lab Método de detección, composición y kit basados en reacciones en cascada de ADN amplificador de señal con extensión de la diana
KR101785074B1 (ko) 2015-01-28 2017-10-12 고려대학교 산학협력단 Dna 합성용 마이크로 칩 및 이를 이용한 dna 합성방법
LT3394093T (lt) 2015-12-23 2022-04-25 Modernatx, Inc. Ox40 ligandus koduojančių polinukleotidų naudojimo būdai
EP3400023A1 (de) 2016-01-10 2018-11-14 ModernaTX, Inc. Therapeutische mrnas zur codierung von anti-ctla-4-antikörpern
HRP20221135T8 (hr) 2016-05-18 2023-02-03 Modernatx, Inc. KOMBINACIJE mRNK KOJE KODIRAJU IMUNOMODULIRAJUĆE POLIPEPTIDE I NJIHOVE UPOTREBE
EP3458083B1 (de) 2016-05-18 2022-11-02 ModernaTX, Inc. Polynukleotide zur codierung von interleukin-12 (il12) und verwendungen davon
CN106916909B (zh) * 2017-05-09 2018-09-11 广州海力特生物科技有限公司 一种HBV pgRNA的实时荧光定量RT-PCR检测引物组、探针组、试剂盒及方法
WO2018231990A2 (en) 2017-06-14 2018-12-20 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
BR112020016306A2 (pt) 2018-02-12 2020-12-15 Curators Of The University Of Missouri Gene (saur) suprarregulado pequeno de auxina para o melhoramento da arquitetura do sistema radicular da planta, tolerância ao encharcamento, resistência à seca, e rendimento
US20220056498A1 (en) 2019-01-25 2022-02-24 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
AU2020400056A1 (en) 2019-12-12 2022-07-07 President And Fellows Of Harvard College Compositions and methods for light-directed biomolecular barcoding
AU2021261343A1 (en) 2020-04-22 2022-11-03 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
EP4225946A4 (de) 2020-10-06 2024-10-30 President And Fellows Of Harvard College Verfahren, zusammensetzungen, kits und systeme für signal-on-nachweis von neutralisierungszielen
WO2025106313A1 (en) 2023-11-14 2025-05-22 Eli Lilly And Company Fc RECEPTOR BASED ASSAYS FOR ANALYZING IgG ANTIBODIES

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
US5225556A (en) * 1985-01-18 1993-07-06 The Trustees Of Columbia University In The City Of New York Chemical probes for left-handed DNA and for A-DNA; chiral metal complexes as Z-specific antitumor agents and as double strand cleavers
DE3601136A1 (de) * 1986-01-16 1987-07-23 Max Planck Gesellschaft Hemmstoffe der reversen transkriptase fuer prophylaxe und therapie von retrovirus-infektionen in saeugetieren
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
US5554498A (en) * 1991-08-30 1996-09-10 Syngenix Limited Nucleic acid amplification using scandium and lanthanum ions
US5418162A (en) * 1991-10-22 1995-05-23 Duke University Serotonin transporter CDNA
AU4248593A (en) * 1992-05-14 1993-12-13 Mark Holodniy Polymerase chain reaction assays for monitoring antiviral therapy
ES2194843T3 (es) * 1992-09-11 2003-12-01 Hoffmann La Roche Deteccion de acidos nucleicos en sangre.
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US6087097A (en) * 1994-05-12 2000-07-11 Mayo Foundation For Medical Education And Research PCR detection of Borrelia burgdorferi
EP0745687A1 (de) 1995-04-08 1996-12-04 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5789206A (en) * 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
AU717156B2 (en) 1995-12-27 2000-03-16 Takara Bio Inc. Novel DNA polymerase
AU720004B2 (en) * 1996-01-26 2000-05-18 Research Institute For Glycotechnology Apoptosis inducers
EP0929694A4 (de) * 1996-03-15 2002-05-02 Penn State Res Found Nachweis von extrazellulärer tumor-assoziierter nukleinsäuren im blutplasma oder -serum mittels eines nukleinsäure-amplifizierungsverfahren
US5917031A (en) * 1996-04-19 1999-06-29 Taiko Pharmaceutical Co., Ltd. Method of synthesizing polydeoxyribonucleotides
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
CN1081903C (zh) 1996-06-12 2002-04-03 宝酒造株式会社 食品或饮料
JP3463780B2 (ja) 1996-07-30 2003-11-05 東洋紡績株式会社 核酸増幅用ｄｎａポリメラーゼ組成物
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
ATE486961T1 (de) * 1997-04-03 2010-11-15 Life Technologies Corp Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr)
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
EP1607480A3 (de) * 1998-09-08 2006-01-11 Takara Bio Inc. Verfahren zur Synthese von DNA
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
JP2001029078A (ja) 1999-07-16 2001-02-06 Shimadzu Corp Ｒｎａ増幅法
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases

Also Published As

Publication number Publication date
EP1816197A2 (de) 2007-08-08
CN1637146A (zh) 2005-07-13
AU3534199A (en) 1999-11-08
CN1306570A (zh) 2001-08-01
US8367328B2 (en) 2013-02-05
EP1072678A1 (de) 2001-01-31
EP1816197A3 (de) 2007-11-28
JP3748378B2 (ja) 2006-02-22
WO1999054455A1 (fr) 1999-10-28
KR100519895B1 (ko) 2005-10-13
JP2006061164A (ja) 2006-03-09
CN1177931C (zh) 2004-12-01
CA2326725A1 (en) 1999-10-28
US7521178B1 (en) 2009-04-21
EP1816197B1 (de) 2009-09-16
EP2116600A2 (de) 2009-11-11
EP2116600B1 (de) 2013-09-18
EP1072678A4 (de) 2006-09-27
EP2116600A3 (de) 2010-01-13
JP4214142B2 (ja) 2009-01-28
DE69941441D1 (de) 2009-10-29
KR20010042958A (ko) 2001-05-25
US20090098613A1 (en) 2009-04-16

Similar Documents

Publication Publication Date Title
DE69941441D1 (de) Methode für DNA synthese
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
CA2310854A1 (en) Isolation and identification of novel polymerases
DE69627698D1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
EP1602725A3 (de) Enzymatische Nukleinsäure die Abasische Nukleotide enthaltet
ATE373672T1 (de) Synthese von methoxynukleoside und enzymatische nukleisäure moleküle
ES2169736T3 (es) Amplificacion de largas secuencias de acidos nucleicos mediante pcr.
HUT77037A (hu) DNS-szekvenálásra alkalmazható, módosított nukleotid-kötőhellyel rendelkező DNS polimerázok
ATE244259T1 (de) Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
WO2001038584A3 (en) Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
ATE426045T1 (de) Prozess zur synthetisierung von nukleinsaure
ATE427992T1 (de) Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
ATE317023T1 (de) Methode zum nachweis von variationen oder polymorphismen
BR9407712A (pt) Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
ATE440962T1 (de) Dns polymerasen
EP1050587A3 (de) Erzeugung und Amplifizierung von Nukleinsäuren ausgehend von Ribonukleinsäuren
ATE299145T1 (de) Neues multimerisierendes reagenz
CA2186021A1 (en) Purified dna polymerase from bacillus stearothermophilus
AU4339196A (en) Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
DE69734604D1 (de) Synthetische polynukleotide
DE69718054D1 (de) Verfahren zur aufreinigung von dns
DK0625194T3 (da) Forøgelse af ribozym-katalytisk aktivitet med et tilstødende facilitator oligonukleotid
DE69926223D1 (de) Verfahren zur herstellung von zwischenprodukten
ATE307199T1 (de) Verfahren und zusammensetzungen zur isolierung und reinigung von nukleinsäuren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties