DE60231149D1 - Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür - Google Patents

Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür

Info

Publication number
DE60231149D1
DE60231149D1 DE60231149T DE60231149T DE60231149D1 DE 60231149 D1 DE60231149 D1 DE 60231149D1 DE 60231149 T DE60231149 T DE 60231149T DE 60231149 T DE60231149 T DE 60231149T DE 60231149 D1 DE60231149 D1 DE 60231149D1
Authority
DE
Germany
Prior art keywords
compositions
enzyme
high accuracy
dna polymerase
accuracy dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60231149T
Other languages
English (en)
Inventor
Holly Hogrefe
Michael Borns
Joseph A Sorge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/035,091 external-priority patent/US9181534B1/en
Priority claimed from US10/227,110 external-priority patent/US7932070B2/en
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of DE60231149D1 publication Critical patent/DE60231149D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
DE60231149T 2001-12-21 2002-12-17 Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür Expired - Lifetime DE60231149D1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10/035,091 US9181534B1 (en) 2001-12-21 2001-12-21 High fidelity DNA polymerase compositions and uses thereof
US7924102A 2002-02-20 2002-02-20
US10/208,508 US20030180741A1 (en) 2001-12-21 2002-07-30 High fidelity DNA polymerase compositions and uses therefor
US10/227,110 US7932070B2 (en) 2001-12-21 2002-08-23 High fidelity DNA polymerase compositions and uses therefor
PCT/US2002/040423 WO2003060144A2 (en) 2001-12-21 2002-12-17 High fidelity dna polymerase compositions and uses therefor

Publications (1)

Publication Number Publication Date
DE60231149D1 true DE60231149D1 (de) 2009-03-26

Family

ID=28044034

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60231149T Expired - Lifetime DE60231149D1 (de) 2001-12-21 2002-12-17 Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür

Country Status (4)

Country Link
US (1) US20030180741A1 (de)
EP (1) EP2110432B1 (de)
AT (1) ATE422537T1 (de)
DE (1) DE60231149D1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1706502A4 (de) * 2003-11-26 2007-05-23 Eppendorf Ag Verfahren und zusammensetzungen zur in-vitro-amplifikation extrachromosomaler nukleinsäure
US7445898B2 (en) * 2004-04-01 2008-11-04 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
EP2208796B1 (de) * 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptide mit Nukleinsäurebindungsaktivität und Zusammensetzungen und Verfahren zur Nukleinsäurenamplifikation
DE102006025153A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerasen mit höherer Selektivität
WO2009085333A1 (en) * 2007-12-27 2009-07-09 Ge Healthcare Bio-Sciences Corp. A single enzyme system for fast, ultra long pcr
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
CN103857805B (zh) * 2011-08-10 2017-07-18 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
WO2014018367A1 (en) * 2012-07-23 2014-01-30 Cynvenio Biosystems, Inc. Dual enzymatic amplification
WO2017058810A2 (en) 2015-10-01 2017-04-06 Life Technologies Corporation Polymerase compositions and kits, and methods of using and making the same
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
KR101958660B1 (ko) * 2017-07-12 2019-03-18 주식회사 진캐스트 유전자 변이 특이성이 증가된 dna 중합효소의 활성 증가용 pcr 버퍼 조성물
US20210324353A1 (en) * 2018-09-03 2021-10-21 Bgi Shenzhen Recombinant kod polymerase
CN115074423A (zh) * 2022-06-27 2022-09-20 爱科睿特生物医疗科技(南京)有限公司 一种pcr缓冲液及其应用

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2511458B1 (fr) 1981-08-14 1986-09-19 Renault Dispositif de filtrage de vibrations et de bruit
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US4946786A (en) 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5352778A (en) 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5210036A (en) 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
WO1992006189A1 (en) 1990-09-27 1992-04-16 Invitrogen Corporation Direct cloning of pcr amplified nucleic acids
DE69132477T2 (de) 1990-09-28 2001-06-13 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5614402A (en) 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US6255062B1 (en) * 1994-02-14 2001-07-03 California Institute Of Technology β-type DNA polymerases
US6100078A (en) 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
CN1123328A (zh) 1994-11-17 1996-05-29 中国科学院上海生物化学研究所 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst)
US5602011A (en) 1995-01-18 1997-02-11 Pharmacia Biotech Inc. Purified Thermococcus barossii DNA polymerase
ATE191740T1 (de) 1995-01-23 2000-04-15 Bayer Ag Gelbildner, brandschutzgele und brandschutzgläser
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
ES2162132T3 (es) 1995-12-15 2001-12-16 Amersham Pharm Biotech Inc Polimerasa de adn termoestable procedente de thermoanaerobacter thermohydrosulfuricus y enzimas mutantes, de actividad exonucleasa suprimida asi obtenidos.
US6183997B1 (en) * 1997-03-21 2001-02-06 Stratagene Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
US6008205A (en) 1997-04-04 1999-12-28 The Brigham & Women's Hospital, Inc. Polyisoprenyl phosphate stable analogs for regulation of neutrophil responses
US6238905B1 (en) 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
US5994079A (en) 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
EP1088891B1 (de) * 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
US20030049614A1 (en) * 1999-10-06 2003-03-13 Holly Hurlbut Hogrefe Compositions for dna amplification, synthesis, and mutagenesis
EP1226255B1 (de) 1999-10-29 2006-03-29 Stratagene California Zusammensetzungen und methoden zur verwendung von dna polymerasen
EP1232270A1 (de) * 1999-11-23 2002-08-21 Amersham Biosciences Corp. Verbesserter dideoxynucleotidtriphosphatverbrauch durch hyperthermophile dna polymerase aus dem archaebakterium i(pyrococcus furiosus)
EP1132474A1 (de) * 2000-03-11 2001-09-12 Roche Diagnostics GmbH B-Typ DNA-Polymerase Mutanten, die eine verbesserte PCR-Leistung aufweisen
JP2001269188A (ja) * 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体

Also Published As

Publication number Publication date
EP2110432A3 (de) 2009-11-11
EP2110432B1 (de) 2015-01-21
ATE422537T1 (de) 2009-02-15
EP2110432A2 (de) 2009-10-21
US20030180741A1 (en) 2003-09-25

Similar Documents

Publication Publication Date Title
WO2003060144A3 (en) High fidelity dna polymerase compositions and uses therefor
DE60231149D1 (de) Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür
DE69637256D1 (de) Synthese von Methoxynukleoside und enzymatische Nukleisäure Moleküle
DE69941441D1 (de) Methode für DNA synthese
DE60230672D1 (de) Nukleinsäureanreicherung
DK1100880T3 (da) Urat oxidase
DE60238493D1 (de) Is von seltenen zellen verwendete markierte zellen
NO20040845L (no) Substituerte indeno[1,2-c]isokinolinderivater og deres anvendelse
DE69808274T2 (de) Kombinationsprodukt, enthaltend eine nukleinsäure und eine substanz, die die extrazellulärematrix desorganisiert, zur verwendung in gentherapie
BR0209149A (pt) Ftalazinonas
ATE478085T1 (de) Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen
DE60225433D1 (de) Verfahren zur herstellung von 1,3-substituierte indene und aryl-annellierte azapolycyclische verbindungen
ATE307845T1 (de) N-alkylaziridinoprepolymere als dentalmasse
DE60209617D1 (de) Quantitative Multiplex-PCR mit grossem Dynamikbereich
ATE498600T1 (de) Inden derivate als pharmazeutika
DK1572959T3 (da) Hybridpolymerase-fremgangsmåder og -sammensætninger
WO2003048309A3 (en) Thermus thermophilus nucleic acid polymerases
DE60239842D1 (de) Verfahren zur Herstellung von 10,11-dihydro-10-oxo-5h-dibenzoÄb,fÜazepin-5-carboxamid
DE60202669D1 (de) 6-phenylbenzonaphthyidine
DE60220621D1 (de) Posh Nukleinsäure, Polypeptide und darauf bezogene Verfahren
HK1066222A1 (en) Mannitol and glucitol derivatives
DE60210184D1 (de) L-cystein herstellendes Bakterium und Verfahren zur Herstellung von L-cystein
DE50201433D1 (de) Haarwachsprodukt mit reduzierter Dichte
DE60106158D1 (de) Verfahren zur Herstellung von Organosilizium-Zusammensetzungen
SE9502259D0 (sv) Oligonucleotides to inhibit the expression of isoprenyl protein transferases

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AGILENT TECHNOLOGIES, INC. (N.D.GES.D. STAATES, US

8328 Change in the person/name/address of the agent

Representative=s name: BARTH, D., DIPL.-ING., PAT.-ANW., 71083 HERRENBERG