ATE422537T1 - Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür - Google Patents

Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür

Info

Publication number
ATE422537T1
ATE422537T1 AT02795911T AT02795911T ATE422537T1 AT E422537 T1 ATE422537 T1 AT E422537T1 AT 02795911 T AT02795911 T AT 02795911T AT 02795911 T AT02795911 T AT 02795911T AT E422537 T1 ATE422537 T1 AT E422537T1
Authority
AT
Austria
Prior art keywords
enzyme
high accuracy
dna polymerase
compositions containing
containing high
Prior art date
Application number
AT02795911T
Other languages
English (en)
Inventor
Holly Hogrefe
Michael Borns
Joseph Sorge
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/035,091 external-priority patent/US9181534B1/en
Priority claimed from US10/227,110 external-priority patent/US7932070B2/en
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of ATE422537T1 publication Critical patent/ATE422537T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02795911T 2001-12-21 2002-12-17 Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür ATE422537T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/035,091 US9181534B1 (en) 2001-12-21 2001-12-21 High fidelity DNA polymerase compositions and uses thereof
US7924102A 2002-02-20 2002-02-20
US10/208,508 US20030180741A1 (en) 2001-12-21 2002-07-30 High fidelity DNA polymerase compositions and uses therefor
US10/227,110 US7932070B2 (en) 2001-12-21 2002-08-23 High fidelity DNA polymerase compositions and uses therefor

Publications (1)

Publication Number Publication Date
ATE422537T1 true ATE422537T1 (de) 2009-02-15

Family

ID=28044034

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02795911T ATE422537T1 (de) 2001-12-21 2002-12-17 Zusammensetzungen mit hochgenauer dna-polymerase und verwendungen dafür

Country Status (4)

Country Link
US (1) US20030180741A1 (de)
EP (1) EP2110432B1 (de)
AT (1) ATE422537T1 (de)
DE (1) DE60231149D1 (de)

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DE102006025153A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerasen mit höherer Selektivität
EP2231878B1 (de) * 2007-12-27 2012-12-26 GE Healthcare Bio-Sciences Corp. Ein-enzym-system für schnelle ultralange pcr
CN107326079B (zh) 2011-08-10 2021-04-20 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP2875147B1 (de) * 2012-07-23 2017-07-05 Cynvenio Biosystems, Inc. Duale enzymatische amplifikation
EP4141126A1 (de) 2015-10-01 2023-03-01 Life Technologies Corporation Polymerasezusammensetzungen und kits sowie verfahren zur verwendung und herstellung davon
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
KR101958660B1 (ko) * 2017-07-12 2019-03-18 주식회사 진캐스트 유전자 변이 특이성이 증가된 dna 중합효소의 활성 증가용 pcr 버퍼 조성물
CN117802065A (zh) * 2018-09-03 2024-04-02 深圳华大生命科学研究院 聚合活性提高的重组型kod聚合酶
CN115074423A (zh) * 2022-06-27 2022-09-20 爱科睿特生物医疗科技(南京)有限公司 一种pcr缓冲液及其应用

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Also Published As

Publication number Publication date
DE60231149D1 (de) 2009-03-26
EP2110432B1 (de) 2015-01-21
EP2110432A2 (de) 2009-10-21
EP2110432A3 (de) 2009-11-11
US20030180741A1 (en) 2003-09-25

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