ATE478085T1 - Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen - Google Patents

Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen

Info

Publication number
ATE478085T1
ATE478085T1 AT01916372T AT01916372T ATE478085T1 AT E478085 T1 ATE478085 T1 AT E478085T1 AT 01916372 T AT01916372 T AT 01916372T AT 01916372 T AT01916372 T AT 01916372T AT E478085 T1 ATE478085 T1 AT E478085T1
Authority
AT
Austria
Prior art keywords
modified oligonucleotides
mismatches
distinction
pyrimidines
substituted
Prior art date
Application number
AT01916372T
Other languages
English (en)
Inventor
Robert Dempcy
Alexander Gall
Sergey Lokhov
Irina Afonina
Michael Singer
Igor Kutyavin
Nicolaas Vermeulen
Original Assignee
Epoch Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/724,959 external-priority patent/US6949367B1/en
Application filed by Epoch Biosciences Inc filed Critical Epoch Biosciences Inc
Application granted granted Critical
Publication of ATE478085T1 publication Critical patent/ATE478085T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT01916372T 2000-03-01 2001-03-01 Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen ATE478085T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18604600P 2000-03-01 2000-03-01
US09/724,959 US6949367B1 (en) 1998-04-03 2000-11-28 Modified oligonucleotides for mismatch discrimination
PCT/US2001/006900 WO2001064958A2 (en) 2000-03-01 2001-03-01 Modified oligonucleotides for mismatch discrimination

Publications (1)

Publication Number Publication Date
ATE478085T1 true ATE478085T1 (de) 2010-09-15

Family

ID=26881723

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01916372T ATE478085T1 (de) 2000-03-01 2001-03-01 Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen

Country Status (8)

Country Link
EP (3) EP1995330A1 (de)
JP (1) JP2003525292A (de)
AT (1) ATE478085T1 (de)
AU (1) AU2001243403A1 (de)
CA (1) CA2401781A1 (de)
DE (1) DE60142828D1 (de)
ES (1) ES2391213T3 (de)
WO (1) WO2001064958A2 (de)

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US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US8569516B2 (en) 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
WO2005040357A2 (en) 2003-10-24 2005-05-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US7381818B2 (en) 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof
WO2008061311A2 (en) * 2006-11-22 2008-05-29 Commonwealth Scientific And Industrial Research Organisation Improved hybridisation of nucleic acids
JP5486501B2 (ja) * 2007-10-09 2014-05-07 イゴール バシリー クートヤビン， 小さなｒｎａ分子の改善された検出のための方法、組成物およびキット
EP3216874A1 (de) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
CA2748174A1 (en) 2008-12-30 2010-07-08 Arqule, Inc. Substituted 1h-pyrazolo[3,4-d]pyrimidine-6-amine compounds
US8343961B2 (en) 2009-03-31 2013-01-01 Arqule, Inc. Substituted heterocyclic compounds
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
EP3572528A1 (de) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direkte erfassung, amplifikation und sequenzierung einer ziel-dna unter verwendung immobilisierter primer
US8362023B2 (en) 2011-01-19 2013-01-29 Hoffmann-La Roche Inc. Pyrazolo pyrimidines
US9677142B2 (en) * 2011-05-24 2017-06-13 Elitechgroup B.V. Detection of methicillin-resistant Staphylococcus aureus
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US20140255928A1 (en) 2013-03-11 2014-09-11 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US9169256B2 (en) 2013-03-13 2015-10-27 Elitechgroup B.V. Artificial nucleic acids
US10738346B2 (en) 2017-03-09 2020-08-11 Elitechgroup, Inc. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
EP3668884B1 (de) 2017-08-17 2022-03-09 ELITechGroup Inc. Duplex stabilisierende fluorescenz quencher für nuklein säure sonden.
EP3553182A1 (de) 2018-04-11 2019-10-16 Université de Bourgogne Verfahren zum nachweis von somatischen genetischen anomalien, kombination von fangsonden und nachweiskit
US11155713B2 (en) 2018-05-29 2021-10-26 Elitechgroup, Inc. Carborhodamine compounds and methods of preparation thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2022040027A1 (en) * 2020-08-17 2022-02-24 University Of Washington Long-term dry storage of enzyme-based reagents in a porous matrix
JP7712517B1 (ja) * 2024-03-19 2025-07-24 防衛装備庁長官 ミスマッチ認識分子、ミスマッチ検出方法、疾病診断方法及びミスマッチ認識分子の製造方法

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Also Published As

Publication number Publication date
AU2001243403A1 (en) 2001-09-12
EP1261616A2 (de) 2002-12-04
WO2001064958A2 (en) 2001-09-07
WO2001064958A3 (en) 2002-03-28
JP2003525292A (ja) 2003-08-26
EP1995330A1 (de) 2008-11-26
EP1975256A1 (de) 2008-10-01
EP1261616B1 (de) 2010-08-18
DE60142828D1 (de) 2010-09-30
EP1975256B1 (de) 2012-07-11
CA2401781A1 (en) 2001-09-07
ES2391213T3 (es) 2012-11-22

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