DE60142828D1 - Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen - Google Patents

Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen

Info

Publication number
DE60142828D1
DE60142828D1 DE60142828T DE60142828T DE60142828D1 DE 60142828 D1 DE60142828 D1 DE 60142828D1 DE 60142828 T DE60142828 T DE 60142828T DE 60142828 T DE60142828 T DE 60142828T DE 60142828 D1 DE60142828 D1 DE 60142828D1
Authority
DE
Germany
Prior art keywords
modified oligonucleotides
malfunctions
distinction
pyrimidines
substituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60142828T
Other languages
English (en)
Inventor
Robert O Dempcy
Alexander A Gall
Sergey G Lokhov
Irina A Afonina
Michael J Singer
Igor V Kutyavin
Nicolaas M Vermeulen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
Epoch Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/724,959 external-priority patent/US6949367B1/en
Application filed by Epoch Biosciences Inc filed Critical Epoch Biosciences Inc
Application granted granted Critical
Publication of DE60142828D1 publication Critical patent/DE60142828D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60142828T 2000-03-01 2001-03-01 Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen Expired - Lifetime DE60142828D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18604600P 2000-03-01 2000-03-01
US09/724,959 US6949367B1 (en) 1998-04-03 2000-11-28 Modified oligonucleotides for mismatch discrimination
PCT/US2001/006900 WO2001064958A2 (en) 2000-03-01 2001-03-01 Modified oligonucleotides for mismatch discrimination

Publications (1)

Publication Number Publication Date
DE60142828D1 true DE60142828D1 (de) 2010-09-30

Family

ID=26881723

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60142828T Expired - Lifetime DE60142828D1 (de) 2000-03-01 2001-03-01 Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen

Country Status (8)

Country Link
EP (3) EP1261616B1 (de)
JP (1) JP2003525292A (de)
AT (1) ATE478085T1 (de)
AU (1) AU2001243403A1 (de)
CA (1) CA2401781A1 (de)
DE (1) DE60142828D1 (de)
ES (1) ES2391213T3 (de)
WO (1) WO2001064958A2 (de)

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US8569516B2 (en) 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
US7541454B2 (en) 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
AU2004286352A1 (en) 2003-10-28 2005-05-12 Epoch Biosciences, Inc. Fluorescent probes for DNA detection by hybridization with improved sensitivity and low background
EP2087133A4 (de) * 2006-11-22 2010-07-21 Commw Scient Ind Res Org Verbesserte hybridisierung von nukleinsäuren
EP2198059B1 (de) 2007-10-09 2014-12-03 Igor Vassily Kutyavin VERFAHREN FÜR DEN VERBESSERTEN NACHWEIS VON sRNA-MOLEKÜLEN
GB2467691A (en) 2008-09-05 2010-08-11 Aueon Inc Methods for stratifying and annotating cancer drug treatment options
WO2010078430A1 (en) 2008-12-30 2010-07-08 Arqule, Inc. Substituted 1h-pyrazolo[3,4-d]pyrimidine-6-amine compounds
US8343961B2 (en) 2009-03-31 2013-01-01 Arqule, Inc. Substituted heterocyclic compounds
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
WO2012040387A1 (en) 2010-09-24 2012-03-29 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US8362023B2 (en) 2011-01-19 2013-01-29 Hoffmann-La Roche Inc. Pyrazolo pyrimidines
DK2714939T3 (en) * 2011-05-24 2015-06-29 Elitech Holding Bv DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS AUREUS
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US9169256B2 (en) 2013-03-13 2015-10-27 Elitechgroup B.V. Artificial nucleic acids
US10738346B2 (en) 2017-03-09 2020-08-11 Elitechgroup, Inc. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
WO2019036225A1 (en) 2017-08-17 2019-02-21 Elitechgroup, Inc. FLUORESCENCE EXTINGUISHERS STABILIZING DUPLEX FOR NUCLEIC PROBES
EP3553182A1 (de) 2018-04-11 2019-10-16 Université de Bourgogne Verfahren zum nachweis von somatischen genetischen anomalien, kombination von fangsonden und nachweiskit
WO2019231617A1 (en) 2018-05-29 2019-12-05 Elitechgroup, Inc. Carborhodamine compounds and methods of preparation thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification

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Also Published As

Publication number Publication date
EP1261616B1 (de) 2010-08-18
EP1995330A1 (de) 2008-11-26
WO2001064958A2 (en) 2001-09-07
CA2401781A1 (en) 2001-09-07
WO2001064958A3 (en) 2002-03-28
EP1975256A1 (de) 2008-10-01
ATE478085T1 (de) 2010-09-15
JP2003525292A (ja) 2003-08-26
ES2391213T3 (es) 2012-11-22
AU2001243403A1 (en) 2001-09-12
EP1261616A2 (de) 2002-12-04
EP1975256B1 (de) 2012-07-11

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