DE60209617D1 - Quantitative Multiplex-PCR mit grossem Dynamikbereich - Google Patents

Quantitative Multiplex-PCR mit grossem Dynamikbereich

Info

Publication number
DE60209617D1
DE60209617D1 DE60209617T DE60209617T DE60209617D1 DE 60209617 D1 DE60209617 D1 DE 60209617D1 DE 60209617 T DE60209617 T DE 60209617T DE 60209617 T DE60209617 T DE 60209617T DE 60209617 D1 DE60209617 D1 DE 60209617D1
Authority
DE
Germany
Prior art keywords
dynamic range
multiplex pcr
large dynamic
quantitative multiplex
quantitative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60209617T
Other languages
English (en)
Other versions
DE60209617T2 (de
Inventor
Karim Dr Tabiti
Gisela Betzl
Richie Dr Soong
Randy Rasmussen
Deepika Marine Desilva
John G Ward
Haliegh Page Willward
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Biofire Defense LLC
Original Assignee
Roche Diagnostics GmbH
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH, Idaho Technology Inc filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE60209617D1 publication Critical patent/DE60209617D1/de
Publication of DE60209617T2 publication Critical patent/DE60209617T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60209617T 2001-11-20 2002-11-16 Quantitative Multiplex-PCR mit grossem Dynamikbereich Expired - Lifetime DE60209617T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33179101P 2001-11-20 2001-11-20
US331791P 2001-11-20

Publications (2)

Publication Number Publication Date
DE60209617D1 true DE60209617D1 (de) 2006-05-04
DE60209617T2 DE60209617T2 (de) 2006-11-09

Family

ID=23295393

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60209617T Expired - Lifetime DE60209617T2 (de) 2001-11-20 2002-11-16 Quantitative Multiplex-PCR mit grossem Dynamikbereich

Country Status (7)

Country Link
US (1) US7118867B2 (de)
EP (1) EP1312684B1 (de)
JP (1) JP3909010B2 (de)
AT (1) ATE319859T1 (de)
CA (1) CA2407744C (de)
DE (1) DE60209617T2 (de)
ES (1) ES2260378T3 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070298423A1 (en) * 2000-03-24 2007-12-27 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US20060216737A1 (en) * 2005-03-10 2006-09-28 John Bodeau Methods for multiplex amplification
US7875274B2 (en) * 2005-12-16 2011-01-25 The Board Of Trustees Of The Leland Stanford Junior University Protein modulators of resistance to alkylating agents
JP4968577B2 (ja) * 2006-04-11 2012-07-04 エフ．ホフマン−ラ ロシュ アーゲー サイトケラチン１９（ＣＫ１９）ｍＲＮＡの迅速測定法、並びにそのためのプライマー及びプローブ
EP2220227B1 (de) * 2007-07-13 2014-09-10 The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense Einmalige kalibrator-polynukleotide und verfahren zur verwendung bei quantitativen nukleinsäure-tests
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
EP2245197B1 (de) * 2008-02-07 2016-10-12 Whitespace Enterprise Corporation Verbesserungen bei und in verbindung mit der analyse
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
EP2393596B1 (de) 2009-02-09 2016-09-28 Whitespace Enterprise Corporation Mikrofluidische vorrichtungen und verfahren zur bereitstellung einer archivierungsprobe
WO2010123626A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms and expression predict clinical outcome in patients with cancer
US20120288861A1 (en) 2009-12-21 2012-11-15 Heinz-Josef Lenz Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
AU2011315926B2 (en) 2010-10-14 2015-12-10 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US9957558B2 (en) 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
US20130059738A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
CN107119137B (zh) * 2017-05-26 2018-04-06 广州华弘生物科技有限公司 一种快速检测HER‑2/neu基因表达的检测试剂盒
CN113462761B (zh) * 2021-07-30 2024-02-09 宁波胤瑞生物医学仪器有限责任公司 一种检测her2基因的引物探针组合物及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006736A1 (en) * 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US5976842A (en) * 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
GB9815799D0 (en) * 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression

Also Published As

Publication number Publication date
US20030215830A1 (en) 2003-11-20
DE60209617T2 (de) 2006-11-09
US7118867B2 (en) 2006-10-10
JP3909010B2 (ja) 2007-04-25
JP2003210199A (ja) 2003-07-29
ES2260378T3 (es) 2006-11-01
ATE319859T1 (de) 2006-03-15
EP1312684A2 (de) 2003-05-21
EP1312684B1 (de) 2006-03-08
EP1312684A3 (de) 2004-02-04
CA2407744A1 (en) 2003-05-20
CA2407744C (en) 2012-01-03

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