ATE427361T1 - Verfahren zur dna-amplifikation und - sequenzierung - Google Patents

Verfahren zur dna-amplifikation und - sequenzierung

Info

Publication number
ATE427361T1
ATE427361T1 AT99903891T AT99903891T ATE427361T1 AT E427361 T1 ATE427361 T1 AT E427361T1 AT 99903891 T AT99903891 T AT 99903891T AT 99903891 T AT99903891 T AT 99903891T AT E427361 T1 ATE427361 T1 AT E427361T1
Authority
AT
Austria
Prior art keywords
thp
dna
sequencing
tetrahydropyrimidine
proline
Prior art date
Application number
AT99903891T
Other languages
English (en)
Inventor
Aviva Lapidot
Robert Iakobashvili
Gennady Malin
Original Assignee
Yeda Res & Dev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev filed Critical Yeda Res & Dev
Application granted granted Critical
Publication of ATE427361T1 publication Critical patent/ATE427361T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
AT99903891T 1998-02-10 1999-02-08 Verfahren zur dna-amplifikation und - sequenzierung ATE427361T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IL12325698A IL123256A0 (en) 1998-02-10 1998-02-10 Methods for dna amplification and sequencing

Publications (1)

Publication Number Publication Date
ATE427361T1 true ATE427361T1 (de) 2009-04-15

Family

ID=11071218

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99903891T ATE427361T1 (de) 1998-02-10 1999-02-08 Verfahren zur dna-amplifikation und - sequenzierung

Country Status (8)

Country Link
US (3) US6428986B1 (de)
EP (1) EP1053350B1 (de)
JP (1) JP4393700B2 (de)
AT (1) ATE427361T1 (de)
DE (1) DE69940662D1 (de)
ES (1) ES2323561T3 (de)
IL (1) IL123256A0 (de)
WO (1) WO1999041410A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL123256A0 (en) * 1998-02-10 1998-09-24 Yeda Res & Dev Methods for dna amplification and sequencing
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7166745B1 (en) 1998-11-12 2007-01-23 Invitrogen Corporation Transfection reagents
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US7101111B2 (en) * 1999-07-19 2006-09-05 Exodyne Technologies Inc. Flared energy absorbing system and method
US7306397B2 (en) * 2002-07-22 2007-12-11 Exodyne Technologies, Inc. Energy attenuating safety system
WO2001023618A2 (en) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity
CN1326990A (zh) * 2000-06-07 2001-12-19 上海博德基因开发有限公司 一种新的多肽——人类dna cgg重复结合蛋白16.17和编码这种多肽的多核苷酸
AU2003272174A1 (en) * 2002-10-21 2004-05-04 Biotage Ab Use of osmolytes to heat stabilise luciferase and/or apyrase
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
US20060193688A1 (en) * 2003-03-05 2006-08-31 Albritton James R Flared Energy Absorbing System and Method
JP2006525023A (ja) * 2003-04-29 2006-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸配列の多重開始増幅
EP1530065B1 (de) * 2003-11-06 2008-09-10 Rohm and Haas Electronic Materials, L.L.C. Optischer Gegenstand mit leitender Struktur
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
JP4896006B2 (ja) 2004-04-08 2012-03-14 バイオマトリカ， インコーポレイテッド ライフサイエンスのためのサンプル保存とサンプル管理との統合
ATE551433T1 (de) 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
ITMI20051050A1 (it) * 2005-06-07 2006-12-08 Roberto Sapio "metodo per la determinazione delle sequenze di acidi nucleici e analoghi"
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP2057289B1 (de) * 2006-08-09 2016-08-10 Univ Singapore Verfahren für molekularbiologische anwendungen
WO2010065629A2 (en) * 2008-12-02 2010-06-10 Kapabiosystems Nucleic acid amplification
US8519125B2 (en) * 2009-05-11 2013-08-27 Biomatrica, Inc. Compositions and methods for biological sample storage
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
SG192862A1 (en) 2011-03-11 2013-09-30 Univ Singapore Pericyte progenitors from peripheral blood
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
EP2935664A4 (de) * 2012-12-21 2016-08-17 Ibis Biosciences Inc Stabilisierte nanoporen- und mikroporenstrukturen sowie verfahren zur herstellung und verwendung davon
CN106572650B (zh) 2014-06-10 2021-08-31 生物马特里卡公司 在环境温度下稳定凝血细胞
CN106572974B (zh) 2014-07-15 2021-04-23 生命技术公司 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法
EP4242628A3 (de) 2015-12-08 2023-11-08 Biomatrica, INC. Verringerung der erythrozytensedimentationsrate
KR102441122B1 (ko) * 2020-05-11 2022-09-07 주식회사 엔지노믹스 폴리머라제 활성 측정 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL100810A (en) 1992-01-30 1996-12-05 Yeda Res & Dev Pharmaceutical preparations including 2 - methyl - carboxy - 5 - hydroxy - tetrahydropyrimidine and / or 2 - methyl - 4 - carboxy - tetrahydropyrimidine, plywood methods
US5432065A (en) 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
JP3616394B2 (ja) * 1994-01-31 2005-02-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｄｎａ配列決定のアーティファクトの除去のための方法
US5545539A (en) 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
FR2733515B1 (fr) * 1995-04-27 1997-09-05 Cis Bio Int Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications
GB9524974D0 (en) * 1995-12-06 1996-02-07 Amersham Int Plc An improved method for dye-terminator (cycle) sequencing
EP1002126A4 (de) 1997-06-25 2004-11-03 Life Technologies Inc Verbessertes verfahren zur isolierung und wiedergewinnung von ziel-dna oder -rna molekülen mit einer gewünschten nukleotidsequenz
IL123256A0 (en) * 1998-02-10 1998-09-24 Yeda Res & Dev Methods for dna amplification and sequencing

Also Published As

Publication number Publication date
IL123256A0 (en) 1998-09-24
JP4393700B2 (ja) 2010-01-06
EP1053350A1 (de) 2000-11-22
EP1053350B1 (de) 2009-04-01
JP2002503482A (ja) 2002-02-05
US6428986B1 (en) 2002-08-06
US20070212760A1 (en) 2007-09-13
DE69940662D1 (de) 2009-05-14
US7150980B1 (en) 2006-12-19
ES2323561T3 (es) 2009-07-20
US7387878B2 (en) 2008-06-17
WO1999041410A1 (en) 1999-08-19

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