DK1171632T3 - Nucleinsyreligand-inhibitorer af DNA-polymeraser - Google Patents

Nucleinsyreligand-inhibitorer af DNA-polymeraser

Info

Publication number
DK1171632T3
DK1171632T3 DK00914690.3T DK00914690T DK1171632T3 DK 1171632 T3 DK1171632 T3 DK 1171632T3 DK 00914690 T DK00914690 T DK 00914690T DK 1171632 T3 DK1171632 T3 DK 1171632T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
dna polymerases
acid ligand
ligands
ligand inhibitors
Prior art date
Application number
DK00914690.3T
Other languages
English (en)
Inventor
Sumedha Jayasena
Larry Gold
Original Assignee
Gilead Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilead Sciences Inc filed Critical Gilead Sciences Inc
Application granted granted Critical
Publication of DK1171632T3 publication Critical patent/DK1171632T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
DK00914690.3T 1999-03-01 2000-02-24 Nucleinsyreligand-inhibitorer af DNA-polymeraser DK1171632T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/258,797 US6183967B1 (en) 1995-06-07 1999-03-01 Nucleic acid ligand inhibitors to DNA polymerases
PCT/US2000/004702 WO2000052207A1 (en) 1999-03-01 2000-02-24 Nucleic acid ligand inhibitors to dna polymerases

Publications (1)

Publication Number Publication Date
DK1171632T3 true DK1171632T3 (da) 2010-11-08

Family

ID=22982164

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00914690.3T DK1171632T3 (da) 1999-03-01 2000-02-24 Nucleinsyreligand-inhibitorer af DNA-polymeraser

Country Status (12)

Country Link
US (1) US6183967B1 (da)
EP (2) EP1171632B1 (da)
JP (2) JP4738599B2 (da)
AT (1) ATE478157T1 (da)
AU (1) AU781567B2 (da)
CA (1) CA2362526A1 (da)
DE (1) DE60044845D1 (da)
DK (1) DK1171632T3 (da)
ES (1) ES2349213T3 (da)
HK (1) HK1044803B (da)
PT (1) PT1171632E (da)
WO (1) WO2000052207A1 (da)

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US6830902B1 (en) * 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
EP2325337A1 (en) * 2000-09-26 2011-05-25 Duke University RNA aptamers and methods for identifying the same
IL159053A0 (en) * 2001-05-25 2004-05-12 Univ Duke Modulators of pharmacological agents
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
WO2004068111A2 (en) * 2003-01-29 2004-08-12 Quanta Biosciences, Inc. Methods and compositions for rna detection and quantitation
CA2521127C (en) 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
US7727969B2 (en) * 2003-06-06 2010-06-01 Massachusetts Institute Of Technology Controlled release nanoparticle having bound oligonucleotide for targeted delivery
EP2460811A1 (en) * 2004-04-22 2012-06-06 Regado Biosciences, Inc. Improved modulators of coagulation factors
CN101426908B (zh) * 2004-06-09 2014-06-11 纽星实验室 可逆性修饰的热稳定酶组合物及其制备方法和使用方法
US20060019288A1 (en) * 2004-06-30 2006-01-26 Applera Corporation Methods, reaction mixtures, and kits for ligating polynucleotides
ES2380775T3 (es) * 2004-10-18 2012-05-18 Brandeis University Reactivos y procedimientos para mejorar la reproducibilidad y reducir el cebado defectuoso en la amplificación por PCR
WO2006074233A2 (en) 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
CN101495657B (zh) * 2006-07-31 2016-08-10 毕万里 使用可逆修饰寡核苷酸扩增核酸
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
ES2647587T3 (es) 2007-07-17 2017-12-22 Somalogic, Inc. Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9637780B2 (en) * 2012-06-11 2017-05-02 Hanwha Techwin Co., Ltd. Controlled inhibition and re-activation of DNA polymerases by cleavable oligonucleotide inhibitors
WO2015081229A2 (en) * 2013-11-26 2015-06-04 Lc Sciences Llc Selective amplification of nucleic acid sequences
CN103966184A (zh) * 2014-05-06 2014-08-06 江西省科学院微生物研究所 一种实时热启动Taq酶及其制备方法
JP7106461B2 (ja) 2016-06-07 2022-07-26 セファイド 熱安定性ポリメラーゼ阻害剤組成物及び方法
CN107619430B (zh) * 2017-10-17 2021-01-15 江西省科学院微生物研究所 一种热稳定的Taq酶温控亲和性配体及其制备方法、应用
WO2020010124A2 (en) * 2018-07-02 2020-01-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
WO2021259201A1 (zh) 2020-06-22 2021-12-30 苏州新海生物科技股份有限公司 一种核酸配体及其应用
KR102661292B1 (ko) * 2021-09-09 2024-04-30 포항공과대학교 산학협력단 금속 이온 반응성 dna 중합 효소 스위치 및 이에 의해 제어되는 등온 증폭 방법
CN114214298A (zh) * 2021-12-30 2022-03-22 苏州译酶生物科技有限公司 一种热启动Taq DNA聚合酶的制备方法
CN115044588B (zh) * 2022-06-16 2023-04-25 湖北省疾病预防控制中心（湖北省预防医学科学院） 一种核酸适配体及热启动dna聚合酶

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WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
JP2763958B2 (ja) 1990-06-11 1998-06-11 ネクスター ファーマスーティカルズ，インコーポレイテッド 核酸リガンド
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Also Published As

Publication number Publication date
AU3604700A (en) 2000-09-21
HK1044803A1 (en) 2002-11-01
AU781567B2 (en) 2005-06-02
PT1171632E (pt) 2010-11-24
US6183967B1 (en) 2001-02-06
JP2002537825A (ja) 2002-11-12
JP2011103890A (ja) 2011-06-02
HK1044803B (zh) 2011-04-29
ATE478157T1 (de) 2010-09-15
EP2241635A1 (en) 2010-10-20
EP1171632A4 (en) 2005-03-30
JP4738599B2 (ja) 2011-08-03
EP1171632A1 (en) 2002-01-16
CA2362526A1 (en) 2000-09-08
WO2000052207A1 (en) 2000-09-08
EP1171632B1 (en) 2010-08-18
ES2349213T3 (es) 2010-12-29
WO2000052207A9 (en) 2001-10-25
DE60044845D1 (de) 2010-09-30

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