PT1171632E - Inibidores ligandos de ácido nucleico para adn-polimerases - Google Patents

Inibidores ligandos de ácido nucleico para adn-polimerases Download PDF

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Publication number
PT1171632E
PT1171632E PT00914690T PT00914690T PT1171632E PT 1171632 E PT1171632 E PT 1171632E PT 00914690 T PT00914690 T PT 00914690T PT 00914690 T PT00914690 T PT 00914690T PT 1171632 E PT1171632 E PT 1171632E
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PT
Portugal
Prior art keywords
seq
polymerase
dna
nucleic acid
ligands
Prior art date
Application number
PT00914690T
Other languages
English (en)
Portuguese (pt)
Inventor
Larry Gold
Sumedha Jayasena
Original Assignee
Gilead Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilead Sciences Inc filed Critical Gilead Sciences Inc
Publication of PT1171632E publication Critical patent/PT1171632E/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
PT00914690T 1999-03-01 2000-02-24 Inibidores ligandos de ácido nucleico para adn-polimerases PT1171632E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/258,797 US6183967B1 (en) 1995-06-07 1999-03-01 Nucleic acid ligand inhibitors to DNA polymerases

Publications (1)

Publication Number Publication Date
PT1171632E true PT1171632E (pt) 2010-11-24

Family

ID=22982164

Family Applications (1)

Application Number Title Priority Date Filing Date
PT00914690T PT1171632E (pt) 1999-03-01 2000-02-24 Inibidores ligandos de ácido nucleico para adn-polimerases

Country Status (12)

Country Link
US (1) US6183967B1 (da)
EP (2) EP1171632B1 (da)
JP (2) JP4738599B2 (da)
AT (1) ATE478157T1 (da)
AU (1) AU781567B2 (da)
CA (1) CA2362526A1 (da)
DE (1) DE60044845D1 (da)
DK (1) DK1171632T3 (da)
ES (1) ES2349213T3 (da)
HK (1) HK1044803B (da)
PT (1) PT1171632E (da)
WO (1) WO2000052207A1 (da)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6830902B1 (en) * 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
WO2002026932A2 (en) * 2000-09-26 2002-04-04 Duke University Rna aptamers and methods for identifying the same
DK1401853T3 (da) * 2001-05-25 2010-11-01 Univ Duke Modulatorer af farmakologiske midler
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
EP1594982A4 (en) * 2003-01-29 2006-04-12 Quanta Biosciences Inc METHODS AND COMPOSITIONS FOR RNA DETECTION AND QUANTIFICATION
WO2004090153A2 (en) 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
US7727969B2 (en) * 2003-06-06 2010-06-01 Massachusetts Institute Of Technology Controlled release nanoparticle having bound oligonucleotide for targeted delivery
JP4718541B2 (ja) 2004-04-22 2011-07-06 リガド・バイオサイエンシーズ・インコーポレーテツド 改良された凝固因子調節剤
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
US20060019288A1 (en) * 2004-06-30 2006-01-26 Applera Corporation Methods, reaction mixtures, and kits for ligating polynucleotides
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
EP2813581B1 (en) * 2005-01-06 2018-06-27 Applied Biosystems, LLC Use of polypeptides having nucleic acid binding activity in methods for fast nucleic acid amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
JP5438320B2 (ja) 2005-10-03 2014-03-12 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 核酸を増幅するための組成物、方法およびキット
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
JP2009545316A (ja) * 2006-07-31 2009-12-24 ワンリ ビ 可逆的に修飾されたオリゴヌクレオチドを使用する核酸増幅
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
ES2647587T3 (es) 2007-07-17 2017-12-22 Somalogic, Inc. Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
US8236570B2 (en) * 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9637780B2 (en) * 2012-06-11 2017-05-02 Hanwha Techwin Co., Ltd. Controlled inhibition and re-activation of DNA polymerases by cleavable oligonucleotide inhibitors
US11473140B2 (en) * 2013-11-26 2022-10-18 Lc Sciences Lc Highly selective omega primer amplification of nucleic acid sequences
CN103966184A (zh) * 2014-05-06 2014-08-06 江西省科学院微生物研究所 一种实时热启动Taq酶及其制备方法
CA3026646A1 (en) 2016-06-07 2017-12-14 Cepheid Thermostable polymerase inhibitor compositions and methods
CN107619430B (zh) * 2017-10-17 2021-01-15 江西省科学院微生物研究所 一种热稳定的Taq酶温控亲和性配体及其制备方法、应用
WO2020010124A2 (en) * 2018-07-02 2020-01-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
CN115135664A (zh) * 2020-06-22 2022-09-30 苏州新海生物科技股份有限公司 一种核酸配体及其应用
KR102661292B1 (ko) * 2021-09-09 2024-04-30 포항공과대학교 산학협력단 금속 이온 반응성 dna 중합 효소 스위치 및 이에 의해 제어되는 등온 증폭 방법
CN114214298A (zh) * 2021-12-30 2022-03-22 苏州译酶生物科技有限公司 一种热启动Taq DNA聚合酶的制备方法
CN115044588B (zh) * 2022-06-16 2023-04-25 湖北省疾病预防控制中心（湖北省预防医学科学院） 一种核酸适配体及热启动dna聚合酶

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4434585A (en) 1985-03-30 1986-10-23 Marc Ballivet Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5874557A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5543293A (en) 1990-06-11 1996-08-06 Nexstar Pharmaceuticals, Inc. DNA ligands of thrombin
US5476766A (en) 1990-06-11 1995-12-19 Nexstar Pharmaceuticals, Inc. Ligands of thrombin
US5683867A (en) 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5472841A (en) 1990-06-11 1995-12-05 Nexstar Pharmaceuticals, Inc. Methods for identifying nucleic acid ligands of human neutrophil elastase
US5459015A (en) 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5587468A (en) 1990-06-11 1996-12-24 University Research Corporation High affinity nucleic acid ligands to HIV integrase
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5527894A (en) 1990-06-11 1996-06-18 Nexstar Pharmacueticals, Inc. Ligands of HIV-1 tat protein
RU2198931C2 (ru) 1990-06-11 2003-02-20 Нексстар Фармасьютикалс Инк. Способ идентификации лигандов, способ получения антитела, способ отбора нуклеиновой кислоты, способ получения смеси нуклеиновых кислот, смесь нуклеиновых кислот, не встречающийся в природе нуклеиново-кислотный лиганд
US5763173A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5503978A (en) 1990-06-11 1996-04-02 University Research Corporation Method for identification of high affinity DNA ligands of HIV-1 reverse transcriptase
US5637459A (en) 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chimeric selex
WO1992014843A1 (en) 1991-02-21 1992-09-03 Gilead Sciences, Inc. Aptamer specific for biomolecules and method of making
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5567604A (en) 1993-04-23 1996-10-22 Aronex Pharmaceuticals, Inc. Anti-viral guanosine-rich oligonucleotides
PT833944E (pt) * 1995-06-07 2009-04-14 Gilead Sciences Inc Ligandos de ácido nucleico que se ligam a, e inibem, adn-polimerases
US20170110787A1 (en) 2015-10-14 2017-04-20 Apple Inc. Electronic Devices With Millimeter Wave Antennas And Metal Housings

Also Published As

Publication number Publication date
EP1171632B1 (en) 2010-08-18
AU781567B2 (en) 2005-06-02
AU3604700A (en) 2000-09-21
CA2362526A1 (en) 2000-09-08
EP1171632A4 (en) 2005-03-30
EP1171632A1 (en) 2002-01-16
HK1044803B (zh) 2011-04-29
DE60044845D1 (de) 2010-09-30
WO2000052207A9 (en) 2001-10-25
ES2349213T3 (es) 2010-12-29
WO2000052207A1 (en) 2000-09-08
DK1171632T3 (da) 2010-11-08
ATE478157T1 (de) 2010-09-15
JP2011103890A (ja) 2011-06-02
JP2002537825A (ja) 2002-11-12
JP4738599B2 (ja) 2011-08-03
US6183967B1 (en) 2001-02-06
HK1044803A1 (en) 2002-11-01
EP2241635A1 (en) 2010-10-20

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