ATE478157T1 - Nukleinsäure-ligand-inhibitoren gegen dna polymerasen - Google Patents

Nukleinsäure-ligand-inhibitoren gegen dna polymerasen

Info

Publication number
ATE478157T1
ATE478157T1 AT00914690T AT00914690T ATE478157T1 AT E478157 T1 ATE478157 T1 AT E478157T1 AT 00914690 T AT00914690 T AT 00914690T AT 00914690 T AT00914690 T AT 00914690T AT E478157 T1 ATE478157 T1 AT E478157T1
Authority
AT
Austria
Prior art keywords
nucleic acid
dna polymerases
acid ligand
inhibitors against
against dna
Prior art date
Application number
AT00914690T
Other languages
English (en)
Inventor
Sumedha Jayasena
Larry Gold
Original Assignee
Gilead Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilead Sciences Inc filed Critical Gilead Sciences Inc
Application granted granted Critical
Publication of ATE478157T1 publication Critical patent/ATE478157T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00914690T 1999-03-01 2000-02-24 Nukleinsäure-ligand-inhibitoren gegen dna polymerasen ATE478157T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/258,797 US6183967B1 (en) 1995-06-07 1999-03-01 Nucleic acid ligand inhibitors to DNA polymerases
PCT/US2000/004702 WO2000052207A1 (en) 1999-03-01 2000-02-24 Nucleic acid ligand inhibitors to dna polymerases

Publications (1)

Publication Number Publication Date
ATE478157T1 true ATE478157T1 (de) 2010-09-15

Family

ID=22982164

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00914690T ATE478157T1 (de) 1999-03-01 2000-02-24 Nukleinsäure-ligand-inhibitoren gegen dna polymerasen

Country Status (12)

Country Link
US (1) US6183967B1 (de)
EP (2) EP1171632B1 (de)
JP (2) JP4738599B2 (de)
AT (1) ATE478157T1 (de)
AU (1) AU781567B2 (de)
CA (1) CA2362526A1 (de)
DE (1) DE60044845D1 (de)
DK (1) DK1171632T3 (de)
ES (1) ES2349213T3 (de)
HK (1) HK1044803B (de)
PT (1) PT1171632E (de)
WO (1) WO2000052207A1 (de)

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IL159053A0 (en) 2001-05-25 2004-05-12 Univ Duke Modulators of pharmacological agents
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
EP1594982A4 (de) * 2003-01-29 2006-04-12 Quanta Biosciences Inc Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna
CA2521127C (en) * 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
US7727969B2 (en) * 2003-06-06 2010-06-01 Massachusetts Institute Of Technology Controlled release nanoparticle having bound oligonucleotide for targeted delivery
PT1745062E (pt) * 2004-04-22 2014-07-11 Regado Biosciences Inc Moduladores melhorados de fatores de coagulação
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
EP1761637A2 (de) * 2004-06-30 2007-03-14 Applera Corporation Verfahren, reaktionsmischungen und kits zur bindung von polynukleotiden
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
AU2006204006A1 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
CN106566855B (zh) * 2006-07-31 2021-11-09 苏州新海生物科技股份有限公司 使用可逆修饰寡核苷酸扩增核酸
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
CA2696431C (en) 2007-07-17 2021-01-05 Somalogic, Inc. Method for generating aptamers with improved off-rates
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
KR20130138700A (ko) * 2012-06-11 2013-12-19 삼성테크윈 주식회사 절단가능한 올리고뉴클레오티드 저해제에 의한 ｄｎａ 폴리머라제의 제어된 저해 및 재활성화
CN111100912B (zh) * 2013-11-26 2023-06-30 杭州联川基因诊断技术有限公司 测定使用的杂交结构
CN103966184A (zh) * 2014-05-06 2014-08-06 江西省科学院微生物研究所 一种实时热启动Taq酶及其制备方法
EP3464596B1 (de) 2016-06-07 2025-07-30 Cepheid Thermostabile polymeraseinhibitorzusammensetzungen und verfahren
CN107619430B (zh) * 2017-10-17 2021-01-15 江西省科学院微生物研究所 一种热稳定的Taq酶温控亲和性配体及其制备方法、应用
WO2020010124A2 (en) * 2018-07-02 2020-01-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
JP2023531027A (ja) * 2020-06-22 2023-07-20 ▲蘇▼州新海生物科技股▲ふん▼有限公司 核酸リガンド、およびその使用
AU2020460256B2 (en) * 2020-07-29 2025-06-05 Mgi Tech Co., Ltd. Method for loading nucleic acid molecule on solid support
KR102661292B1 (ko) * 2021-09-09 2024-04-30 포항공과대학교 산학협력단 금속 이온 반응성 dna 중합 효소 스위치 및 이에 의해 제어되는 등온 증폭 방법
CN114214298A (zh) * 2021-12-30 2022-03-22 苏州译酶生物科技有限公司 一种热启动Taq DNA聚合酶的制备方法
CN115044588B (zh) * 2022-06-16 2023-04-25 湖北省疾病预防控制中心（湖北省预防医学科学院） 一种核酸适配体及热启动dna聚合酶
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

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WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
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Also Published As

Publication number Publication date
JP2002537825A (ja) 2002-11-12
AU781567B2 (en) 2005-06-02
DK1171632T3 (da) 2010-11-08
JP4738599B2 (ja) 2011-08-03
EP1171632B1 (de) 2010-08-18
AU3604700A (en) 2000-09-21
DE60044845D1 (de) 2010-09-30
EP2241635A1 (de) 2010-10-20
PT1171632E (pt) 2010-11-24
HK1044803A1 (en) 2002-11-01
WO2000052207A9 (en) 2001-10-25
EP1171632A1 (de) 2002-01-16
WO2000052207A1 (en) 2000-09-08
ES2349213T3 (es) 2010-12-29
US6183967B1 (en) 2001-02-06
JP2011103890A (ja) 2011-06-02
EP1171632A4 (de) 2005-03-30
CA2362526A1 (en) 2000-09-08
HK1044803B (en) 2011-04-29

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