BR0014182A - Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas - Google Patents

Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas

Info

Publication number
BR0014182A
BR0014182A BR0014182-8A BR0014182A BR0014182A BR 0014182 A BR0014182 A BR 0014182A BR 0014182 A BR0014182 A BR 0014182A BR 0014182 A BR0014182 A BR 0014182A
Authority
BR
Brazil
Prior art keywords
primer
composite
compositions
processes
methods
Prior art date
Application number
BR0014182-8A
Other languages
English (en)
Inventor
Nurith Kurn
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Publication of BR0014182A publication Critical patent/BR0014182A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Amplifiers (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Patente de Invenção: "PROCESSOS E COMPOSIçõES PARA AMPLIFICAçãO ISOTéRMICA LINEAR DE SEQuêNCIAS POLINUCLEOTìDICAS". A presente invenção fornece novos processos de amplificação de ácidos nucléicos lineares isotérmicos com um único iniciador. São fornecidos processos para a amplificação de DNA complementar utilizando um iniciador compósito, a extensão do iniciador, o deslocamento do filamento e opcionalmente uma seq³ência de terminação. São também fornecidos métodos para a amplificação de RNA sentido utilizando um iniciador compósito, a extensão do iniciador, o deslocamento do filamento, opcionalmente a troca de molde, um oligonucleotídeo pró-promotor e a transcrição. A invenção fornece ainda composições e kits para a prática dos ditos processos, assim como os processos que utilizam os produtos de amplificação.
BR0014182-8A 1999-09-13 2000-09-13 Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas BR0014182A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15360499P 1999-09-13 1999-09-13
US17578000P 2000-01-12 2000-01-12
PCT/US2000/025104 WO2001020035A2 (en) 1999-09-13 2000-09-13 Methods and compositions for linear isothermal amplification of polynucleotide sequences

Publications (1)

Publication Number Publication Date
BR0014182A true BR0014182A (pt) 2002-05-21

Family

ID=26850696

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014182-8A BR0014182A (pt) 1999-09-13 2000-09-13 Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas

Country Status (19)

Country Link
US (2) US6251639B1 (pt)
EP (2) EP1218542B1 (pt)
JP (2) JP3929775B2 (pt)
KR (1) KR100527265B1 (pt)
CN (2) CN102586228A (pt)
AT (1) ATE262595T1 (pt)
AU (1) AU783873B2 (pt)
BR (1) BR0014182A (pt)
CA (1) CA2384838C (pt)
DE (1) DE60009323T2 (pt)
DK (2) DK1431303T3 (pt)
ES (2) ES2214319T3 (pt)
HK (1) HK1046021B (pt)
IL (1) IL148091A0 (pt)
MX (1) MXPA02002656A (pt)
NO (1) NO20021223L (pt)
NZ (1) NZ517121A (pt)
PT (1) PT1218542E (pt)
WO (1) WO2001020035A2 (pt)

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