DK1543438T3 - Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler - Google Patents

Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler

Info

Publication number
DK1543438T3
DK1543438T3 DK03754518T DK03754518T DK1543438T3 DK 1543438 T3 DK1543438 T3 DK 1543438T3 DK 03754518 T DK03754518 T DK 03754518T DK 03754518 T DK03754518 T DK 03754518T DK 1543438 T3 DK1543438 T3 DK 1543438T3
Authority
DK
Denmark
Prior art keywords
methods
melting temperature
systems
algorithms
estimating
Prior art date
Application number
DK03754518T
Other languages
English (en)
Inventor
Richard Owczarzy
Joseph Alan Walder
Lingyan Huang
Mark Aaron Behlke
Original Assignee
Integrated Dna Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc filed Critical Integrated Dna Tech Inc
Application granted granted Critical
Publication of DK1543438T3 publication Critical patent/DK1543438T3/da

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Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

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  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Biophysics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK03754518T 2002-09-12 2003-09-12 Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler DK1543438T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41066302P 2002-09-12 2002-09-12
PCT/US2003/028664 WO2004025257A2 (en) 2002-09-12 2003-09-12 Methods and systems for estimating the melting temperature (tm) for polynucleotide molecules

Publications (1)

Publication Number Publication Date
DK1543438T3 true DK1543438T3 (da) 2009-08-03

Family

ID=31994175

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03754518T DK1543438T3 (da) 2002-09-12 2003-09-12 Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler

Country Status (9)

Country Link
US (1) US6889143B2 (da)
EP (1) EP1543438B1 (da)
JP (1) JP4455334B2 (da)
AT (1) ATE430964T1 (da)
AU (1) AU2003272340B2 (da)
CA (1) CA2498414A1 (da)
DE (1) DE60327541D1 (da)
DK (1) DK1543438T3 (da)
WO (1) WO2004025257A2 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8055451B2 (en) * 2007-01-05 2011-11-08 Integrated Dna Technologies, Inc. Method for estimating a melting temperature of a nucleic acid in buffers containing magnesium ions
JP5294380B2 (ja) * 2007-12-05 2013-09-18 学校法人東京理科大学 核酸構造解析装置及びプログラム
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
US20110117559A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
WO2012018697A1 (en) 2010-08-02 2012-02-09 Integrated Dna Technologies, Inc. Methods for predicting stability and melting temperatures of nucleic acid duplexes
US11330838B2 (en) 2019-07-19 2022-05-17 R. J. Reynolds Tobacco Company Holder for aerosol delivery device with detachable cartridge

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3855864T2 (de) 1987-11-30 1997-09-25 Univ Iowa Res Found Durch modifikationen an der 3'-terminalen phosphodiesterbindung stabilisierte dna moleküle, ihre verwendung als nukleinsäuresonden sowie als therapeutische mittel zur hemmung der expression spezifischer zielgene
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
EP1103910A1 (en) * 1999-11-24 2001-05-30 Schütz, Ekkehard Method of automatically selecting Oligonucleotide Hybridization probes

Also Published As

Publication number Publication date
EP1543438B1 (en) 2009-05-06
ATE430964T1 (de) 2009-05-15
EP1543438A2 (en) 2005-06-22
US20040115705A1 (en) 2004-06-17
WO2004025257A2 (en) 2004-03-25
JP2006502711A (ja) 2006-01-26
EP1543438A4 (en) 2006-04-12
US6889143B2 (en) 2005-05-03
CA2498414A1 (en) 2004-03-25
AU2003272340B2 (en) 2009-08-13
DE60327541D1 (de) 2009-06-18
AU2003272340A1 (en) 2004-04-30
WO2004025257A3 (en) 2004-08-12
JP4455334B2 (ja) 2010-04-21

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