AU2003272174A1 - Use of osmolytes to heat stabilise luciferase and/or apyrase - Google Patents

Use of osmolytes to heat stabilise luciferase and/or apyrase

Info

Publication number
AU2003272174A1
AU2003272174A1 AU2003272174A AU2003272174A AU2003272174A1 AU 2003272174 A1 AU2003272174 A1 AU 2003272174A1 AU 2003272174 A AU2003272174 A AU 2003272174A AU 2003272174 A AU2003272174 A AU 2003272174A AU 2003272174 A1 AU2003272174 A1 AU 2003272174A1
Authority
AU
Australia
Prior art keywords
osmolytes
apyrase
stabilise
luciferase
heat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003272174A
Inventor
Pal Nyren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotage AB
Original Assignee
Biotage AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0203089A external-priority patent/SE0203089D0/en
Application filed by Biotage AB filed Critical Biotage AB
Publication of AU2003272174A1 publication Critical patent/AU2003272174A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2003272174A 2002-10-21 2003-10-21 Use of osmolytes to heat stabilise luciferase and/or apyrase Abandoned AU2003272174A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US31963502P 2002-10-21 2002-10-21
US60/319,635 2002-10-21
SE0203089-8 2002-10-21
SE0203089A SE0203089D0 (en) 2002-10-21 2002-10-21 New use
PCT/SE2003/001627 WO2004035818A1 (en) 2002-10-21 2003-10-21 Use of osmolytes to heat stabilise luciferase and/or apyrase

Publications (1)

Publication Number Publication Date
AU2003272174A1 true AU2003272174A1 (en) 2004-05-04

Family

ID=32109588

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003272174A Abandoned AU2003272174A1 (en) 2002-10-21 2003-10-21 Use of osmolytes to heat stabilise luciferase and/or apyrase

Country Status (2)

Country Link
AU (1) AU2003272174A1 (en)
WO (1) WO2004035818A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2499480A (en) 2010-03-31 2013-08-21 Stabilitech Ltd Method for preserving alum adjuvants and alum-adjuvanted vaccines
EP2552478B1 (en) 2010-03-31 2016-12-21 Stabilitech Ltd. Excipients for stabilising viral particles
JP5960120B2 (en) 2010-03-31 2016-08-02 スタビリテック リミテッド Stabilization of virus particles
GB201117233D0 (en) 2011-10-05 2011-11-16 Stabilitech Ltd Stabilisation of polypeptides
GB201406569D0 (en) 2014-04-11 2014-05-28 Stabilitech Ltd Vaccine compositions
JP6698651B2 (en) 2014-11-13 2020-05-27 スリーエム イノベイティブ プロパティズ カンパニー Kit containing ATP-diphosphohydrolase for detecting bacterial ATP in a sample
GB2562241B (en) 2017-05-08 2022-04-06 Stabilitech Biopharma Ltd Vaccine compositions
CN110607347A (en) * 2019-10-30 2019-12-24 浙江东鸿生物科技有限公司 ATP fluorescence detection reagent
CN116445433B (en) * 2023-06-09 2023-08-11 北京纳百生物科技有限公司 Novel North American firefly luciferase variant and ATP fluorescence detection reagent using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH088864B2 (en) * 1988-04-12 1996-01-31 キッコーマン株式会社 Luciferase
US5908751A (en) * 1996-04-26 1999-06-01 Toyo Ink Mfg. Co., Ltd. Method for detecting and/or determining ATP from microorganism cells in a sample
WO1998055653A1 (en) * 1997-06-06 1998-12-10 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
IL123256A0 (en) * 1998-02-10 1998-09-24 Yeda Res & Dev Methods for dna amplification and sequencing
US6406891B1 (en) * 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme
US20010046687A1 (en) * 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system

Also Published As

Publication number Publication date
WO2004035818A1 (en) 2004-04-29

Similar Documents

Publication Publication Date Title
AU2003219987A1 (en) Electroluminescent materials and methods of manufacture and use
HK1067945A1 (en) Substituted benzazoles and use thereof as raf kinase inhibitors
AU2003287160A1 (en) Substituted indoles and their use as hcv inhibitors
AU2003216482A8 (en) Msras as modifiers of the p53 pathway and methods of use
AU2003226941A1 (en) Antifouling composition comprising an enzyme in the absence of its substrate
AU2003256297A1 (en) Benzimidazoles and benzothiazoles as inhibitors of map kinase
AU2003272174A1 (en) Use of osmolytes to heat stabilise luciferase and/or apyrase
AU2003276604A1 (en) Manipulation of objects with fluid droplets
EP1613628A4 (en) Reversible inhibitors of s-adenosyl-l-homocysteine hydrolase and uses thereof
AU2003294716A1 (en) Detectable labeled nucleoside analogs and methods of use thereof
PL378414A1 (en) USE OF α-PHENYLTHIOCARBOXYLIC AND α-PHENYLOXYCARBOXYLIC ACIDS WITH SERUM-GLUCOSE-LOWERING AND SERUM-LIPID-LOWERING ACTIVITY
AU2003230844A1 (en) Crystals and structures of pak4kd kinase pak4kd
GB2393571B (en) Improvements in and relating to the control of instruments
AU2003218715A1 (en) Azepane derivatives and their use as atk1 inhibitors
AU2003272390A1 (en) AMPDs AS MODIFIERS OF THE p21 PATHWAY AND METHODS OF USE
AU2003251799A1 (en) Mchks as modifiers of the chk1 pathway and methods of use
AU2003269334A1 (en) Crystal structure of staphylococcus undecaprenyl pyrophosphate synthase and uses thereof
AU2003274911A1 (en) Mrbs as modifiers of the rb pathway and methods of use
AU2003265263A1 (en) ADCY3S AS MODIFIERS OF THE p21 PATHWAY AND METHODS OF USE
AU2003254471A1 (en) Improvements in and relating to the measurement of joint laxity
AU2003215725A1 (en) Materials and methods relating to the treatment of lymphoma
AU2003231531A1 (en) Kinases and phosphatases
EP1578945A3 (en) Cct6s as modifiers of the rb pathway and methods of use
AU2003294499A1 (en) Rhebs as modifiers of the rb pathway and methods of use
AU2003251826A1 (en) Mp21s as modifiers of the p21 pathway and methods of use

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase