DE69628959D1 - Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation - Google Patents

Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation

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Publication number
DE69628959D1
DE69628959D1 DE69628959T DE69628959T DE69628959D1 DE 69628959 D1 DE69628959 D1 DE 69628959D1 DE 69628959 T DE69628959 T DE 69628959T DE 69628959 T DE69628959 T DE 69628959T DE 69628959 D1 DE69628959 D1 DE 69628959D1
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Germany
Prior art keywords
nucleic acid
acid amplification
compositions
reverse transcriptase
rna polymerase
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69628959T
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English (en)
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DE69628959T2 (de
Inventor
Nancy Lau Liu Shen
Daniel Louis Kacian
James Garfield Putnam
William Michael Davis
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Gen Probe Inc
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Gen Probe Inc
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Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE69628959D1 publication Critical patent/DE69628959D1/de
Application granted granted Critical
Publication of DE69628959T2 publication Critical patent/DE69628959T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69628959T 1995-02-10 1996-02-09 Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation Expired - Lifetime DE69628959T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US387011 1995-02-10
US08/387,011 US5556771A (en) 1995-02-10 1995-02-10 Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Publications (2)

Publication Number Publication Date
DE69628959D1 true DE69628959D1 (de) 2003-08-14
DE69628959T2 DE69628959T2 (de) 2004-05-13

Family

ID=23528056

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69628959T Expired - Lifetime DE69628959T2 (de) 1995-02-10 1996-02-09 Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation

Country Status (10)

Country Link
US (3) US5556771A (de)
EP (1) EP0726310B1 (de)
JP (1) JP3282819B2 (de)
KR (1) KR19980702110A (de)
AT (1) ATE244759T1 (de)
AU (1) AU699590B2 (de)
CA (1) CA2210584A1 (de)
DE (1) DE69628959T2 (de)
ES (1) ES2202387T3 (de)
WO (1) WO1996024664A1 (de)

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AU699590B2 (en) 1998-12-10
WO1996024664A1 (en) 1996-08-15
AU4916796A (en) 1996-08-27
US5834254A (en) 1998-11-10
US5614387A (en) 1997-03-25
ATE244759T1 (de) 2003-07-15
JP3282819B2 (ja) 2002-05-20
ES2202387T3 (es) 2004-04-01
KR19980702110A (ko) 1998-07-15
EP0726310B1 (de) 2003-07-09
US5556771A (en) 1996-09-17
DE69628959T2 (de) 2004-05-13
JPH10503383A (ja) 1998-03-31
CA2210584A1 (en) 1996-08-15

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