CN103687960A - 反转录聚合酶链式反应的方法 - Google Patents

反转录聚合酶链式反应的方法 Download PDF

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Publication number
CN103687960A
CN103687960A CN201180071676.4A CN201180071676A CN103687960A CN 103687960 A CN103687960 A CN 103687960A CN 201180071676 A CN201180071676 A CN 201180071676A CN 103687960 A CN103687960 A CN 103687960A
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China
Prior art keywords
capillary
polymerase chain
chain reaction
reverse transcription
reactant
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苏城
邓秉华
李馥君
吴思颖
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Genereach Biotechnology Corp
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Genereach Biotechnology Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

本发明提供一种反转录聚合酶链式反应的方法，其主要包括以下步骤：准备毛细管，将反转录反应所需的反转录酶加入毛细管中，经过预冻干燥程序，在该毛细管内制成反转录冻干反应剂，由此，在使用时可将RNA检体、缓冲液、聚合酶与引物溶液直接加入该毛细管中，与反转录冻干反应剂混合进行回溶，使反转录反应与聚合酶链式反应可一并于毛细管内进行，因而有助于提高实验流程的便利性。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201180071676.4A 2011-07-21 2011-07-21 反转录聚合酶链式反应的方法 Pending CN103687960A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2011/077449 WO2013010333A1 (zh) 2011-07-21 2011-07-21 反转录聚合酶链式反应的方法

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CN103687960A true CN103687960A (zh) 2014-03-26

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US (1) US20130023010A1 (zh)
CN (1) CN103687960A (zh)
WO (1) WO2013010333A1 (zh)

Families Citing this family (3)

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CL2015000980A1 (es) 2014-04-18 2016-07-01 Upstartdna Co Ltd Standard Entity Métodos para detectar patógenos en peces de agua fría.
CN109439733A (zh) * 2018-10-17 2019-03-08 东南大学 一种基于细长反应腔的rna转录组分析方法
CN112295628A (zh) * 2019-08-01 2021-02-02 利多(香港)有限公司 试剂的保存方法

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US20090011488A1 (en) * 2003-08-18 2009-01-08 Rosetta Inpharmatics, Llc Methods for storing compositions useful for synthesizing nucleic acid molecules
CN101812539B (zh) * 2009-12-03 2012-07-04 中国兽医药品监察所 猪瘟病毒TaqMan-MGB荧光定量RT-PCR鉴别检测试剂盒及其生产方法
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0479158A1 (en) * 1990-09-29 1992-04-08 Shimadzu Corporation Reagent for RNA detection and RNA detection method using it
EP0726310A1 (en) * 1995-02-10 1996-08-14 Gen-Probe Incorporated Stabilized enzyme compositons for nucleic acid amplification
EP0834729A2 (en) * 1996-09-26 1998-04-08 Becton, Dickinson and Company DNA microwell device and method
US20070243601A1 (en) * 2004-06-04 2007-10-18 Teemu Korpimaki Method for Stabilizing Assay Reagents, Reagent Container with Stabilized Assay Reagents and Use Thereof
US20060068399A1 (en) * 2004-09-24 2006-03-30 Cepheid Multiple bead reagent system for protein based assays with optimized matrices
CN101983236A (zh) * 2008-01-24 2011-03-02 基亚生物科技股份有限公司 热对流聚合酶连锁反应之方法及装置

Non-Patent Citations (2)

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Title
CHRISTIANE GOBLET,ET AL: "One-step amplification of transcripts in totoal RNA using the polymerase chain reaction", 《NUCLEIC ACIDS RESEARCH》, vol. 17, no. 5, 31 December 1989 (1989-12-31), pages 2144, XP001120560 *
WEN PIN CHOU,ET AL: "Rapid DNA amplification in a capillary tube by natural convection with a single isothermal heater", 《BIOTECHNIQUES》, vol. 50, 31 January 2011 (2011-01-31), pages 52 - 57, XP055170196, DOI: 10.2144/000113589 *

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US20130023010A1 (en) 2013-01-24
WO2013010333A1 (zh) 2013-01-24

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Application publication date: 20140326