MX2009007280A - Reactivos estables y equipos utiles en la amplificacion isotermica mediada por la espiral (lamp). - Google Patents

Reactivos estables y equipos utiles en la amplificacion isotermica mediada por la espiral (lamp).

Info

Publication number
MX2009007280A
MX2009007280A MX2009007280A MX2009007280A MX2009007280A MX 2009007280 A MX2009007280 A MX 2009007280A MX 2009007280 A MX2009007280 A MX 2009007280A MX 2009007280 A MX2009007280 A MX 2009007280A MX 2009007280 A MX2009007280 A MX 2009007280A
Authority
MX
Mexico
Prior art keywords
loop
isothermal amplification
mediated isothermal
lamp
kits useful
Prior art date
Application number
MX2009007280A
Other languages
English (en)
Inventor
Todd Denison Pack
Xiaokang Deng
Original Assignee
Meridian Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meridian Bioscience Inc filed Critical Meridian Bioscience Inc
Publication of MX2009007280A publication Critical patent/MX2009007280A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/625—Detection means characterised by use of a special device being a nucleic acid test strip device, e.g. dipsticks, strips, tapes, CD plates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporciona en la presente una preparación de reactivos para la amplificación isotérmica mediada por la espiral de ácidos nucleicos que comprende: por lo menos una enzima de polimerasa, un conjunto de cebadores específicos del objetivo y trifosfatos de dinucleótido (dNTPs) en un solo formato seco; en donde la preparación de reactivos es soluble en agua y estable arriba de 4ºC.
MX2009007280A 2007-01-17 2008-01-16 Reactivos estables y equipos utiles en la amplificacion isotermica mediada por la espiral (lamp). MX2009007280A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88098807P 2007-01-17 2007-01-17
PCT/US2008/051229 WO2008089286A1 (en) 2007-01-17 2008-01-16 Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)

Publications (1)

Publication Number Publication Date
MX2009007280A true MX2009007280A (es) 2009-09-08

Family

ID=39365596

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009007280A MX2009007280A (es) 2007-01-17 2008-01-16 Reactivos estables y equipos utiles en la amplificacion isotermica mediada por la espiral (lamp).

Country Status (9)

Country Link
US (1) US20080182312A1 (es)
EP (1) EP2108048A1 (es)
KR (1) KR20090100460A (es)
CN (1) CN101605911A (es)
AU (1) AU2008206221A1 (es)
CA (1) CA2673772A1 (es)
MX (1) MX2009007280A (es)
WO (1) WO2008089286A1 (es)
ZA (1) ZA200904497B (es)

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AP2011005669A0 (en) * 2008-09-15 2011-04-30 Univ Witwatersrand Jhb Primer and amplification method for forming singlestranded DNA amplicons.
JP5572943B2 (ja) * 2008-12-17 2014-08-20 株式会社島津製作所 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット
ES2351294B8 (es) * 2009-07-02 2012-06-06 Consejo Superior De Investigaciones Científicas (Csic) Metodo para la replicación, amplificación, o secuenciación de un adn molde.
AT510418B1 (de) * 2011-05-06 2012-04-15 Univ Wien Tech Lamp-verfahren zum nachweisen von erwinia amylovora
US9523120B2 (en) 2011-06-14 2016-12-20 University of Pittsburgh—of the Commonwealth System of Higher Education Method of amplifying a nucleic acid
WO2013067272A1 (en) * 2011-11-04 2013-05-10 Diagnostics For All, Inc. Low cost, disposable molecular diagnostic devices
KR102398399B1 (ko) 2013-08-09 2022-05-16 루미넥스 코포레이션 핵산 에세이에서의 향상된 용융 식별 및 복합화를 위한 프로브
CA2956812C (en) 2014-08-11 2023-04-11 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109694927A (zh) * 2019-03-01 2019-04-30 纽奥维特(成都)生物科技有限公司 一种hpv病毒的引物体系及其检测方法和应用
US11898197B2 (en) 2020-07-30 2024-02-13 Wainamics, Inc. System and self-metering cartridges for point of care bioassays
US11904314B2 (en) 2020-07-30 2024-02-20 Wainamics, Inc. System and self-metering cartridges for point of care bioassays
WO2022271743A1 (en) * 2021-06-21 2022-12-29 The Johns Hopkins University Rapid lamp methods for detecting bacterial and viral pathogens

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US3825304A (en) 1971-05-27 1974-07-23 Sauget A Method of electrically controlling braking of a vehicle and an electronically controlled braking device for a vehicle
US4629689A (en) * 1984-08-29 1986-12-16 Allied Corporation Binding assay with amplified read-out and gas-phase detection
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
DE69034177T2 (de) * 1989-07-11 2005-10-27 Gen-Probe Inc., San Diego Verfahren zur Amplifikation von Nukleinsäuresequenzen
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
WO1995003430A1 (en) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
CA2185239C (en) * 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5814506A (en) * 1995-08-02 1998-09-29 New England Biolabs, Inc. Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion
US5830714A (en) * 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
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Also Published As

Publication number Publication date
EP2108048A1 (en) 2009-10-14
CA2673772A1 (en) 2008-07-24
KR20090100460A (ko) 2009-09-23
ZA200904497B (en) 2010-04-28
AU2008206221A1 (en) 2008-07-24
WO2008089286A1 (en) 2008-07-24
US20080182312A1 (en) 2008-07-31
CN101605911A (zh) 2009-12-16

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