DE60139331D1 - Verfahren zur polynukleotidsynthese - Google Patents
Verfahren zur polynukleotidsyntheseInfo
- Publication number
- DE60139331D1 DE60139331D1 DE60139331T DE60139331T DE60139331D1 DE 60139331 D1 DE60139331 D1 DE 60139331D1 DE 60139331 T DE60139331 T DE 60139331T DE 60139331 T DE60139331 T DE 60139331T DE 60139331 D1 DE60139331 D1 DE 60139331D1
- Authority
- DE
- Germany
- Prior art keywords
- polynucleotide synthesis
- loops
- polynucleotides
- isothermal
- synthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000283862 | 2000-09-19 | ||
PCT/JP2001/008142 WO2002024902A1 (fr) | 2000-09-19 | 2001-09-19 | Procede permettant de synthetiser un polynucleotide |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60139331D1 true DE60139331D1 (de) | 2009-09-03 |
Family
ID=18768163
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60139331T Expired - Lifetime DE60139331D1 (de) | 2000-09-19 | 2001-09-19 | Verfahren zur polynukleotidsynthese |
Country Status (10)
Country | Link |
---|---|
US (3) | US7374913B2 (de) |
EP (2) | EP2031058B1 (de) |
JP (2) | JP4918210B2 (de) |
CN (1) | CN1222614C (de) |
AT (1) | ATE437225T1 (de) |
AU (1) | AU2001288065A1 (de) |
DE (1) | DE60139331D1 (de) |
ES (2) | ES2327712T3 (de) |
HK (1) | HK1055445A1 (de) |
WO (1) | WO2002024902A1 (de) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8445664B2 (en) * | 1998-06-24 | 2013-05-21 | Enzo Diagnostics, Inc. | Kits for amplifying and detecting nucleic acid sequences |
US7374913B2 (en) * | 2000-09-19 | 2008-05-20 | Eiken Kagaku Kabushiki Kaisha | Method for synthesizing polynucleotides |
ES2382868T3 (es) | 2002-02-20 | 2012-06-14 | Sysmex Corporation | Cebadores para amplificación de ácido nucleico en la detección ARNm de gen constitutivo y método de ensayo que usa estos cebadores |
ATE516365T1 (de) | 2002-05-21 | 2011-07-15 | Sysmex Corp | Nukleinsäureamplifikationsprimer zum nachweis von cytokeratinen und untersuchungsverfahren mit verwendung der primer |
US7803579B2 (en) * | 2002-10-29 | 2010-09-28 | Riken | Process for amplifying nucleic acid |
US7399588B2 (en) | 2003-06-27 | 2008-07-15 | Eiken Kagaku Kabushiki Kaisha | Method for detecting SARS coronavirus |
JP2005110587A (ja) * | 2003-10-08 | 2005-04-28 | Sapporo Breweries Ltd | アリサイクロバチラス属菌検出用プライマー |
US8206902B2 (en) * | 2003-12-25 | 2012-06-26 | Riken | Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same |
JP2008000002A (ja) * | 2004-09-30 | 2008-01-10 | Sysmex Corp | リブロース2リン酸カルボキシラーゼスモールチェーン1A(RBCS−1A)遺伝子及び/又は該遺伝子のmRNAを検出するための核酸増幅用プライマ、及び内部標準として該遺伝子及び/又は該遺伝子のmRNAを用いた検査方法。 |
US8043813B2 (en) | 2004-11-01 | 2011-10-25 | Japan As Represented By Director-General Of National Institute Of Infectious Diseases | Method of detecting H5 or H7 avian influenza virus |
JP2006254784A (ja) * | 2005-03-17 | 2006-09-28 | Institute Of Physical & Chemical Research | プライマーダイマーからの非特異的増幅を減少させる方法 |
FR2885140B1 (fr) | 2005-04-29 | 2010-12-31 | Millipore Corp | Procede de detection et de caracterisation de microorgarnismes sur une membrane. |
US8956850B2 (en) * | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
WO2007069331A1 (ja) * | 2005-12-16 | 2007-06-21 | Eiken Kagaku Kabushiki Kaisha | ヒト免疫不全ウイルス1型の検出方法 |
JP4580875B2 (ja) * | 2006-01-20 | 2010-11-17 | 株式会社東芝 | 核酸検出法のためのプライマーの設計方法 |
JP4881623B2 (ja) | 2006-01-27 | 2012-02-22 | シスメックス株式会社 | Cea核酸増幅用プライマー、プライマーセット、及びがんの診断支援方法 |
MX2009007280A (es) * | 2007-01-17 | 2009-09-08 | Meridian Bioscience Inc | Reactivos estables y equipos utiles en la amplificacion isotermica mediada por la espiral (lamp). |
CA2622649C (en) | 2007-03-09 | 2018-04-24 | Riken | Nucleic acid amplification method using primer exhibiting exciton effect |
JP4410269B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | N−アセチルトランスフェラーゼ2(nat2)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
JP4410268B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | メチレンテトラヒドロ葉酸還元酵素(mthfr)の遺伝子型を検出するための核酸プライマーセット及び核酸プローブ |
JP4490988B2 (ja) | 2007-04-26 | 2010-06-30 | 株式会社東芝 | 血清アミロイドa1(saa1)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
WO2009054510A1 (ja) | 2007-10-25 | 2009-04-30 | Riken | 等温増幅方法およびそれに用いるdnaポリメラーゼ |
JPWO2009119331A1 (ja) * | 2008-03-28 | 2011-07-21 | シスメックス株式会社 | サイトケラチン7のmRNAの検出用プライマを含む試薬 |
CN103923989B (zh) | 2008-05-28 | 2016-04-27 | 花王株式会社 | 耐热菌类的检测方法 |
EP2325309B1 (de) | 2008-05-29 | 2014-10-22 | Kao Corporation | Verfahren zum nachweis von paecilomyces variotii |
US20110236900A1 (en) | 2008-11-27 | 2011-09-29 | Riken | Novel muts protein and method for determing mutation using the same |
CN102246043B (zh) | 2008-12-25 | 2016-06-08 | 环球生物研究株式会社 | 试样的预处理方法和生物相关物质的测定方法 |
GB0910302D0 (en) * | 2009-06-15 | 2009-07-29 | Lumora Ltd | Nucleic acid amplification |
JP5299981B1 (ja) | 2011-09-08 | 2013-09-25 | 独立行政法人理化学研究所 | プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法 |
WO2013065574A1 (ja) | 2011-10-31 | 2013-05-10 | 栄研化学株式会社 | 標的核酸の検出法 |
WO2014085687A1 (en) | 2012-11-28 | 2014-06-05 | Abwiz Bio, Inc. | Preparation of gene-specific templates for the use in single primer amplification |
US9677981B2 (en) | 2013-03-15 | 2017-06-13 | 3M Innovative Properties Company | Sample concentrator and method of use |
US10351918B2 (en) | 2013-10-30 | 2019-07-16 | Green Life Biotech, Llc | Pathogenesis quantification systems and treatment methods for citrus greening blight |
WO2016011280A1 (en) | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
WO2016073353A1 (en) | 2014-11-03 | 2016-05-12 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
WO2016098892A1 (ja) * | 2014-12-19 | 2016-06-23 | 国立大学法人 長崎大学 | ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法 |
JP6605238B2 (ja) * | 2014-12-19 | 2019-11-13 | 国立大学法人 長崎大学 | ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法 |
MY193444A (en) | 2015-03-30 | 2022-10-13 | Greenlight Biosciences Inc | Cell-free production of ribonucleic acid |
JPWO2017212904A1 (ja) * | 2016-06-06 | 2019-04-11 | 国立大学法人 宮崎大学 | 複数のプライマーセットを組み合わせたlamp法を用いたアフリカ豚コレラウイルスの迅速検出法 |
JP7176682B2 (ja) | 2018-02-22 | 2022-11-22 | 佐藤製薬株式会社 | 白癬菌の遺伝子をlamp法により検出するためのプライマーセット及びそれを含むキット並びにそれらを用いて白癬菌を検出する方法 |
CN109658982B (zh) * | 2018-12-25 | 2020-06-19 | 人和未来生物科技(长沙)有限公司 | 一种用于基因测序的引物设计方法及系统 |
SG10201910254YA (en) | 2019-11-04 | 2021-06-29 | Denka Company Ltd | Nucleic Acid Detection Method Using Lamp And Probe Detection |
CN110878367A (zh) * | 2019-12-05 | 2020-03-13 | 华南农业大学 | 一种可检测snp的新型cpa方法、引物组和试剂盒 |
CN115151656A (zh) | 2020-02-27 | 2022-10-04 | 荣研化学株式会社 | 检测单链rna病毒的方法 |
JP7313537B2 (ja) | 2020-03-05 | 2023-07-24 | 栄研化学株式会社 | コロナウイルス(SARS-CoV-2)の検出方法 |
CN115867653A (zh) | 2020-03-13 | 2023-03-28 | 艾优实验室公司 | 用于检测靶核酸的环状引物和loop-de-loop方法 |
EP4146833A1 (de) | 2020-05-05 | 2023-03-15 | Genomtec SA | Diagnostische primer, kits und verfahren für den nachweis von viren |
WO2021252836A1 (en) | 2020-06-12 | 2021-12-16 | Sherlock Biosciences | Crispr-based sars-cov-2 detection |
WO2022029449A1 (en) | 2020-08-07 | 2022-02-10 | Oxford Nanopore Technologies Limited | Methods of identifying nucleic acid barcodes |
CN112553300A (zh) * | 2020-10-15 | 2021-03-26 | 德歌生物技术(山东)有限公司 | 引物设计方法、引物及等温扩增核酸片段的方法 |
EP3992300A1 (de) | 2020-10-30 | 2022-05-04 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO | Probenvorbehandlung zur isothermen amplifikation |
CA3210883A1 (en) | 2021-02-09 | 2022-08-18 | Sherlock Biosciences, Inc. | Nucleic acid amplification using promoter primers |
US11788129B2 (en) | 2021-02-23 | 2023-10-17 | Industrial Technology Research Institute | Nucleic acid detection kit and nucleic acid detection method |
CN113201583B (zh) * | 2021-04-29 | 2022-02-08 | 国科宁波生命与健康产业研究院 | 恒温条件下合成核酸的方法及试剂盒和应用 |
EP4105327A4 (de) * | 2021-04-29 | 2023-01-18 | Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences | Verfahren zur herstellung von nukleinsäuren mit konstanter temperatur und kit und verwendung davon |
CN118434883A (zh) | 2021-12-24 | 2024-08-02 | 豪夫迈·罗氏有限公司 | Lamp扩增产物的改进检测 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6743605B1 (en) * | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
ES2294862T5 (es) * | 1998-11-09 | 2011-04-12 | Eiken Kagaku Kabushiki Kaisha | Procedimiento de síntesis de ácido nucleico. |
CA2390309C (en) | 1999-11-08 | 2012-09-25 | Eiken Kagaku Kabushiki Kaisha | Method for synthesizing the nucleic acid |
AU1057801A (en) | 1999-11-08 | 2001-06-06 | Eiken Kagaku Kabushiki Kaisha | Method of detecting variation or polymorphism |
US8017357B2 (en) * | 2000-04-07 | 2011-09-13 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
US7374913B2 (en) * | 2000-09-19 | 2008-05-20 | Eiken Kagaku Kabushiki Kaisha | Method for synthesizing polynucleotides |
-
2001
- 2001-09-19 US US10/380,930 patent/US7374913B2/en not_active Expired - Lifetime
- 2001-09-19 ES ES01967728T patent/ES2327712T3/es not_active Expired - Lifetime
- 2001-09-19 AU AU2001288065A patent/AU2001288065A1/en not_active Abandoned
- 2001-09-19 ES ES08019286T patent/ES2390242T3/es not_active Expired - Lifetime
- 2001-09-19 AT AT01967728T patent/ATE437225T1/de not_active IP Right Cessation
- 2001-09-19 WO PCT/JP2001/008142 patent/WO2002024902A1/ja active Application Filing
- 2001-09-19 EP EP08019286A patent/EP2031058B1/de not_active Expired - Lifetime
- 2001-09-19 EP EP01967728A patent/EP1327679B1/de not_active Expired - Lifetime
- 2001-09-19 JP JP2002529496A patent/JP4918210B2/ja not_active Expired - Lifetime
- 2001-09-19 DE DE60139331T patent/DE60139331D1/de not_active Expired - Lifetime
- 2001-09-19 CN CNB018190839A patent/CN1222614C/zh not_active Expired - Lifetime
-
2003
- 2003-10-24 HK HK03107694.1A patent/HK1055445A1/xx not_active IP Right Cessation
-
2008
- 2008-04-15 US US12/103,492 patent/US7851186B2/en not_active Expired - Fee Related
- 2008-04-15 US US12/103,506 patent/US7846695B2/en not_active Expired - Fee Related
-
2011
- 2011-12-28 JP JP2011289124A patent/JP2012085652A/ja not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
CN1222614C (zh) | 2005-10-12 |
US20080213842A1 (en) | 2008-09-04 |
CN1474870A (zh) | 2004-02-11 |
JPWO2002024902A1 (ja) | 2004-01-29 |
EP2031058A1 (de) | 2009-03-04 |
EP1327679A4 (de) | 2005-06-01 |
US7846695B2 (en) | 2010-12-07 |
EP2031058B1 (de) | 2012-07-18 |
US7374913B2 (en) | 2008-05-20 |
EP1327679B1 (de) | 2009-07-22 |
ES2390242T3 (es) | 2012-11-07 |
JP4918210B2 (ja) | 2012-04-18 |
ATE437225T1 (de) | 2009-08-15 |
US20080213790A1 (en) | 2008-09-04 |
US20040038253A1 (en) | 2004-02-26 |
JP2012085652A (ja) | 2012-05-10 |
US7851186B2 (en) | 2010-12-14 |
WO2002024902A1 (fr) | 2002-03-28 |
ES2327712T3 (es) | 2009-11-03 |
EP1327679A1 (de) | 2003-07-16 |
HK1055445A1 (en) | 2004-01-09 |
AU2001288065A1 (en) | 2002-04-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60139331D1 (de) | Verfahren zur polynukleotidsynthese | |
BR9808368A (pt) | Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. | |
ATE549381T1 (de) | Verfahren zur herstellung von modifizierten pigmenten | |
ATE521718T1 (de) | Prozess zur synthetisierung von nukleinsäure | |
DK1828381T3 (da) | Templatedirigeret split-and-mix-syntese af småmolekylebiblioteker | |
ATE429414T1 (de) | Verfahren zur herstellung von organischen carbonaten | |
ATE264268T1 (de) | Verfahren zur herstellung von zsm-5 und zsm-11 | |
ATE541826T1 (de) | Verfahren zur herstellung von alkylaminen durch umsetzung von olefinen mit ammoniak | |
DE60214864D1 (de) | Verfahren zur herstellung von nukleinsäuren bestehend aus stochastisch kombinierten anteilen von ausgangsnukleinsäuren | |
ATE525341T1 (de) | Verfahren zur herstellung von 1-acetyl-1- chlorcyclopropan | |
ATE517857T1 (de) | Herstellung von 4-phenylbutansäure | |
DE69909921D1 (de) | Verfahren zur herstellung von n-methyl-pyrrolidon | |
ATE207861T1 (de) | Verfahren zur herstellung von tetrabrombisphenol- a | |
ATE323703T1 (de) | Synthese von verbindungen die zur herstellung von ketorolac dienen | |
DE502005006902D1 (de) | Verfahren zur herstellung von amino- oder hydroxybenzonitrilen | |
ATE318803T1 (de) | Verfahren zur herstellung von laktonen | |
ATE499154T1 (de) | Verfahren zur herstellung von vanadylpyrophosphat-katalysatoren | |
HUP0100943A2 (hu) | Eljárás poliamidok előállítására | |
ATE387424T1 (de) | Verfahren zur herstellung von chlorsulfonylisocyanat | |
ATE98954T1 (de) | Verfahren zur synthese von n,ndialkylhydroxylaminen. | |
ATE527213T1 (de) | Verfahren zur herstellung von ammoniumpolythiomolybdat | |
ATE46190T1 (de) | Gerbstoff und verfahren zu seiner herstellung. | |
ATE469141T1 (de) | Verfahren zur herstellung von substituierten thiophensulfonylisocyanaten | |
ATE384730T1 (de) | Verfahren zur herstellung von palladium(0)- haltigen verbindungen | |
ATE417817T1 (de) | Verfahren zur herstellung eines optisch aktiven amids ausgehend von einem alpha, beta- ungesättigtem amid derivat in anwesenheit eines optisch aktiven übergangsmetallkomplexes, der eine phosphin-phosphoran verbindung und rhodium enthält |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |