ATE437225T1 - Verfahren zur polynukleotidsynthese - Google Patents
Verfahren zur polynukleotidsyntheseInfo
- Publication number
- ATE437225T1 ATE437225T1 AT01967728T AT01967728T ATE437225T1 AT E437225 T1 ATE437225 T1 AT E437225T1 AT 01967728 T AT01967728 T AT 01967728T AT 01967728 T AT01967728 T AT 01967728T AT E437225 T1 ATE437225 T1 AT E437225T1
- Authority
- AT
- Austria
- Prior art keywords
- polynucleotide synthesis
- loops
- polynucleotides
- isothermal
- synthesis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000283862 | 2000-09-19 | ||
PCT/JP2001/008142 WO2002024902A1 (fr) | 2000-09-19 | 2001-09-19 | Procede permettant de synthetiser un polynucleotide |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE437225T1 true ATE437225T1 (de) | 2009-08-15 |
Family
ID=18768163
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01967728T ATE437225T1 (de) | 2000-09-19 | 2001-09-19 | Verfahren zur polynukleotidsynthese |
Country Status (10)
Country | Link |
---|---|
US (3) | US7374913B2 (de) |
EP (2) | EP2031058B1 (de) |
JP (2) | JP4918210B2 (de) |
CN (1) | CN1222614C (de) |
AT (1) | ATE437225T1 (de) |
AU (1) | AU2001288065A1 (de) |
DE (1) | DE60139331D1 (de) |
ES (2) | ES2390242T3 (de) |
HK (1) | HK1055445A1 (de) |
WO (1) | WO2002024902A1 (de) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8445664B2 (en) * | 1998-06-24 | 2013-05-21 | Enzo Diagnostics, Inc. | Kits for amplifying and detecting nucleic acid sequences |
ATE437225T1 (de) * | 2000-09-19 | 2009-08-15 | Eiken Chemical | Verfahren zur polynukleotidsynthese |
JP4426309B2 (ja) | 2002-02-20 | 2010-03-03 | シスメックス株式会社 | ハウスキーピング遺伝子のmRNA検出のための核酸増幅用プライマー及び該プライマーを用いた検査方法 |
EP1510587B1 (de) | 2002-05-21 | 2011-07-13 | Sysmex Corporation | Nukleinsäureamplifikationsprimer zum nachweis von cytokeratinen und untersuchungsverfahren mit verwendung der primer |
CA2504234C (en) * | 2002-10-29 | 2011-09-13 | Riken | Process for amplifying nucleic acids |
JP4603979B2 (ja) * | 2003-06-27 | 2010-12-22 | 栄研化学株式会社 | Sarsコロナウイルスの検出法 |
JP2005110587A (ja) * | 2003-10-08 | 2005-04-28 | Sapporo Breweries Ltd | アリサイクロバチラス属菌検出用プライマー |
JP3897805B2 (ja) * | 2003-12-25 | 2007-03-28 | 独立行政法人理化学研究所 | 核酸の増幅法およびこれを利用した変異核酸の検出法 |
JP2008000002A (ja) * | 2004-09-30 | 2008-01-10 | Sysmex Corp | リブロース2リン酸カルボキシラーゼスモールチェーン1A(RBCS−1A)遺伝子及び/又は該遺伝子のmRNAを検出するための核酸増幅用プライマ、及び内部標準として該遺伝子及び/又は該遺伝子のmRNAを用いた検査方法。 |
ES2382446T3 (es) | 2004-11-01 | 2012-06-08 | Eiken Kagaku Kabushiki Kaisha | Método de detección del virus de la gripe aviar H5 |
JP2006254784A (ja) * | 2005-03-17 | 2006-09-28 | Institute Of Physical & Chemical Research | プライマーダイマーからの非特異的増幅を減少させる方法 |
FR2885140B1 (fr) | 2005-04-29 | 2010-12-31 | Millipore Corp | Procede de detection et de caracterisation de microorgarnismes sur une membrane. |
WO2007069331A1 (ja) * | 2005-12-16 | 2007-06-21 | Eiken Kagaku Kabushiki Kaisha | ヒト免疫不全ウイルス1型の検出方法 |
JP4580875B2 (ja) | 2006-01-20 | 2010-11-17 | 株式会社東芝 | 核酸検出法のためのプライマーの設計方法 |
JP4881623B2 (ja) | 2006-01-27 | 2012-02-22 | シスメックス株式会社 | Cea核酸増幅用プライマー、プライマーセット、及びがんの診断支援方法 |
CA2673772A1 (en) * | 2007-01-17 | 2008-07-24 | Meridian Bioscience, Inc. | Stable reagents and kits useful in loop-mediated isothermal amplification (lamp) |
CA2622649C (en) | 2007-03-09 | 2018-04-24 | Riken | Nucleic acid amplification method using primer exhibiting exciton effect |
JP4410268B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | メチレンテトラヒドロ葉酸還元酵素(mthfr)の遺伝子型を検出するための核酸プライマーセット及び核酸プローブ |
JP4410269B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | N−アセチルトランスフェラーゼ2(nat2)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
JP4490988B2 (ja) | 2007-04-26 | 2010-06-30 | 株式会社東芝 | 血清アミロイドa1(saa1)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
US20100221787A1 (en) | 2007-10-25 | 2010-09-02 | Riken | Isothermal amplification method and dna polymerase used in the same |
EP2284281A4 (de) * | 2008-03-28 | 2011-08-10 | Sysmex Corp | Reagens mit primer zum nachweis von mrna für cytokeratin-7 |
US9074261B2 (en) | 2008-05-28 | 2015-07-07 | Kao Corporation | Method of detecting heat-resistant fungus |
EP2325309B1 (de) | 2008-05-29 | 2014-10-22 | Kao Corporation | Verfahren zum nachweis von paecilomyces variotii |
BRPI0909989A2 (pt) * | 2008-06-05 | 2021-06-22 | Butamax Advanced Biofuels Llc | célula de levedura recombinante e método para a produção de um produto |
JPWO2010061922A1 (ja) | 2008-11-27 | 2012-04-26 | 独立行政法人理化学研究所 | 新規MutSタンパク質およびそれを用いた変異の判定方法 |
SG171899A1 (en) | 2008-12-25 | 2011-07-28 | Universal Bio Research Co Ltd | Method for pretreating specimen and method for assaying biological substance |
GB0910302D0 (en) * | 2009-06-15 | 2009-07-29 | Lumora Ltd | Nucleic acid amplification |
WO2013035875A1 (ja) | 2011-09-08 | 2013-03-14 | 独立行政法人理化学研究所 | プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法 |
WO2013065574A1 (ja) | 2011-10-31 | 2013-05-10 | 栄研化学株式会社 | 標的核酸の検出法 |
CN105026573A (zh) | 2012-11-28 | 2015-11-04 | 艾布维兹生物公司 | 用于单引物扩增的基因特异模板的制备 |
US9677981B2 (en) | 2013-03-15 | 2017-06-13 | 3M Innovative Properties Company | Sample concentrator and method of use |
JP2016538882A (ja) | 2013-10-30 | 2016-12-15 | グリーン ライフ バイオテック,エルエルシー | カンキツグリーニング病のための病因定量システム及び治療方法 |
CN106661600B (zh) | 2014-07-16 | 2021-04-06 | 唐恩生物科技股份有限公司 | 用于扩增核酸样品的等温方法 |
CN107208012B (zh) | 2014-11-03 | 2021-06-29 | 唐恩生物科学公司 | 用于细胞、孢子或病毒捕获和破碎的装置和方法 |
JP6605238B2 (ja) | 2014-12-19 | 2019-11-13 | 国立大学法人 長崎大学 | ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法 |
WO2016098892A1 (ja) * | 2014-12-19 | 2016-06-23 | 国立大学法人 長崎大学 | ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法 |
KR20180002636A (ko) | 2015-03-30 | 2018-01-08 | 그린라이트 바이오사이언시스, 아이엔씨. | 리보핵산의 무세포 생산 |
JPWO2017212904A1 (ja) * | 2016-06-06 | 2019-04-11 | 国立大学法人 宮崎大学 | 複数のプライマーセットを組み合わせたlamp法を用いたアフリカ豚コレラウイルスの迅速検出法 |
JP7176682B2 (ja) | 2018-02-22 | 2022-11-22 | 佐藤製薬株式会社 | 白癬菌の遺伝子をlamp法により検出するためのプライマーセット及びそれを含むキット並びにそれらを用いて白癬菌を検出する方法 |
CN109658982B (zh) * | 2018-12-25 | 2020-06-19 | 人和未来生物科技(长沙)有限公司 | 一种用于基因测序的引物设计方法及系统 |
SG10201910254YA (en) | 2019-11-04 | 2021-06-29 | Denka Company Ltd | Nucleic Acid Detection Method Using Lamp And Probe Detection |
CN110878367A (zh) * | 2019-12-05 | 2020-03-13 | 华南农业大学 | 一种可检测snp的新型cpa方法、引物组和试剂盒 |
US20230175079A1 (en) | 2020-02-27 | 2023-06-08 | Eiken Kagaku Kabushiki Kaisha | Method for detecting single-stranded rna virus |
EP4116438A4 (de) | 2020-03-05 | 2024-04-03 | Eiken Kagaku Kabushiki Kaisha | Verfahren zur detektion von coronavirus (sars-cov-2) |
BR112022018015A2 (pt) | 2020-03-13 | 2022-12-06 | Uh Oh Labs Inc | Iniciador em loop e método loop a loop para detectar ácido nucleico alvo |
US20240279750A1 (en) | 2020-05-05 | 2024-08-22 | Genomtec Sa | Diagnostic Primers, Kits and Methods for Viral Detection |
EP4165218A1 (de) | 2020-06-12 | 2023-04-19 | Sherlock Biosciences, Inc. | Sars-cov-2-detektion auf crispr-basis |
US20220059187A1 (en) | 2020-08-07 | 2022-02-24 | Oxford Nanopore Technologies Limited | Methods of detecting nucleic acid barcodes |
CN112553300A (zh) * | 2020-10-15 | 2021-03-26 | 德歌生物技术(山东)有限公司 | 引物设计方法、引物及等温扩增核酸片段的方法 |
EP3992300A1 (de) | 2020-10-30 | 2022-05-04 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO | Probenvorbehandlung zur isothermen amplifikation |
EP4291678A1 (de) | 2021-02-09 | 2023-12-20 | Sherlock Biosciences, Inc. | Nukleinsäureamplifikation unter verwendung von promotorprimern |
US11788129B2 (en) | 2021-02-23 | 2023-10-17 | Industrial Technology Research Institute | Nucleic acid detection kit and nucleic acid detection method |
EP4105327A4 (de) * | 2021-04-29 | 2023-01-18 | Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences | Verfahren zur herstellung von nukleinsäuren mit konstanter temperatur und kit und verwendung davon |
CN113201583B (zh) * | 2021-04-29 | 2022-02-08 | 国科宁波生命与健康产业研究院 | 恒温条件下合成核酸的方法及试剂盒和应用 |
WO2023118399A1 (en) | 2021-12-24 | 2023-06-29 | F. Hoffmann-La Roche Ag | Improved detection of lamp amplification products |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6743605B1 (en) * | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
EP2045337B1 (de) | 1998-11-09 | 2011-08-24 | Eiken Kagaku Kabushiki Kaisha | Prozess zur Synthetisierung von Nukleinsäure |
BR0015382B1 (pt) | 1999-11-08 | 2014-04-29 | Eiken Chemical | Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia |
EP1231281B1 (de) * | 1999-11-08 | 2006-02-01 | Eiken Kagaku Kabushiki Kaisha | Methode zum nachweis von variationen oder polymorphismen |
EP1275715B1 (de) * | 2000-04-07 | 2015-07-15 | Eiken Kagaku Kabushiki Kaisha | Verfahren zur amplifikation und erkennung von nukleinsäuren unter verwendung von doppelsträngigen nukleinsäuren als schablone |
ATE437225T1 (de) * | 2000-09-19 | 2009-08-15 | Eiken Chemical | Verfahren zur polynukleotidsynthese |
-
2001
- 2001-09-19 AT AT01967728T patent/ATE437225T1/de not_active IP Right Cessation
- 2001-09-19 AU AU2001288065A patent/AU2001288065A1/en not_active Abandoned
- 2001-09-19 WO PCT/JP2001/008142 patent/WO2002024902A1/ja active Application Filing
- 2001-09-19 EP EP08019286A patent/EP2031058B1/de not_active Expired - Lifetime
- 2001-09-19 JP JP2002529496A patent/JP4918210B2/ja not_active Expired - Lifetime
- 2001-09-19 US US10/380,930 patent/US7374913B2/en not_active Expired - Lifetime
- 2001-09-19 ES ES08019286T patent/ES2390242T3/es not_active Expired - Lifetime
- 2001-09-19 ES ES01967728T patent/ES2327712T3/es not_active Expired - Lifetime
- 2001-09-19 DE DE60139331T patent/DE60139331D1/de not_active Expired - Lifetime
- 2001-09-19 EP EP01967728A patent/EP1327679B1/de not_active Expired - Lifetime
- 2001-09-19 CN CNB018190839A patent/CN1222614C/zh not_active Expired - Lifetime
-
2003
- 2003-10-24 HK HK03107694.1A patent/HK1055445A1/xx not_active IP Right Cessation
-
2008
- 2008-04-15 US US12/103,506 patent/US7846695B2/en not_active Expired - Fee Related
- 2008-04-15 US US12/103,492 patent/US7851186B2/en not_active Expired - Fee Related
-
2011
- 2011-12-28 JP JP2011289124A patent/JP2012085652A/ja not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
JP2012085652A (ja) | 2012-05-10 |
US20080213842A1 (en) | 2008-09-04 |
US7374913B2 (en) | 2008-05-20 |
WO2002024902A1 (fr) | 2002-03-28 |
ES2327712T3 (es) | 2009-11-03 |
EP1327679A1 (de) | 2003-07-16 |
JP4918210B2 (ja) | 2012-04-18 |
EP2031058A1 (de) | 2009-03-04 |
HK1055445A1 (en) | 2004-01-09 |
US20080213790A1 (en) | 2008-09-04 |
US7851186B2 (en) | 2010-12-14 |
JPWO2002024902A1 (ja) | 2004-01-29 |
US7846695B2 (en) | 2010-12-07 |
EP1327679A4 (de) | 2005-06-01 |
CN1474870A (zh) | 2004-02-11 |
EP1327679B1 (de) | 2009-07-22 |
CN1222614C (zh) | 2005-10-12 |
DE60139331D1 (de) | 2009-09-03 |
ES2390242T3 (es) | 2012-11-07 |
EP2031058B1 (de) | 2012-07-18 |
AU2001288065A1 (en) | 2002-04-02 |
US20040038253A1 (en) | 2004-02-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |