CY1109828T1 - Κατασκευασμενα προτυπα και η χρηση τους στην ενισχυση μοναδικου εκκινητη - Google Patents

Κατασκευασμενα προτυπα και η χρηση τους στην ενισχυση μοναδικου εκκινητη

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Publication number
CY1109828T1
CY1109828T1 CY20101100051T CY101100051T CY1109828T1 CY 1109828 T1 CY1109828 T1 CY 1109828T1 CY 20101100051 T CY20101100051 T CY 20101100051T CY 101100051 T CY101100051 T CY 101100051T CY 1109828 T1 CY1109828 T1 CY 1109828T1
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CY
Cyprus
Prior art keywords
polynucleotide
template
primer
anneals
predetermined sequence
Prior art date
Application number
CY20101100051T
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English (en)
Inventor
Katherine S Bowdish
Shana Frederickson
Toshiaki Maruyama
Ying-Chi Lin
Mark Renshaw
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Alexion Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharmaceuticals, Inc. filed Critical Alexion Pharmaceuticals, Inc.
Publication of CY1109828T1 publication Critical patent/CY1109828T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Control Of Amplification And Gain Control (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)

Abstract

Μέθοδοι ενίσχυσης νουκλεϊνικού οξέος έχουν ανακαλυφθεί τώρα που περιλαμβάνουν τα βήματα: α) ανόπτηση ενός εκκινητή σε ακολουθία νουκλεϊνικού οξέος προτύπου, με τον εκκινητή να έχει πρώτο τμήμα που υποβάλλεται σε ανόπτηση στο πρότυπο και δεύτερο τμήμα της προκαθορισμένης ακολουθίας· β) σύνθεση πολυνουκλεοτιδίου που υποβάλλεται σε υβριδισμό σε και είναι συμπληρωματικό στο τμήμα του προτύπου μεταξύ της τοποθεσίας στην οποία υποβάλλεται σε ανόπτηση το πρώτο τμήμα του εκκινητή στο πρότυπο και του τέλους του προτύπου, με το πολυνουκλεοτίδιο να έχει πρώτο άκρο και δεύτερο άκρο, όπου το πρώτο άκρο ενσωματώνει τον εκκινητή· γ) διαχωρισμό του πολυνουκλεοτιδίου που συντίθεται στο βήμα (β) από το πρότυπο· δ) ανόπτηση ενός ένθετου ολιγονουκλεοτιδίου στο δεύτερο άκρο του πολυνουκλεοτιδίου που συντέθηκε στη βαθμίδα (β), με το ένθετο ολιγονουκλεοτίδιο να έχει πρώτο τμήμα που υποβάλλεται σε ανόπτηση στο δεύτερο άκρο του πολυνουκλεοτιδίου και δεύτερο τμήμα που έχει την ίδια προκαθορισμένη ακολουθία όπως το δεύτερο τμήμα του εκκινητή· ε) επέκταση του πολυνουκλεοτιδίου που συντέθηκε στο βήμα (β) για παροχή ενός τερματικού τμήματος του που είναι συμπληρωματικό στην προκαθορισμένη ακολουθία· και στ) ενίσχυση του εκτεταμένου πολυνουκλεοτιδίου χρησιμοποιώντας ένα μοναδικό εκκινητή που έχει την προκαθορισμένη ακολουθία.
CY20101100051T 2001-09-19 2010-01-18 Κατασκευασμενα προτυπα και η χρηση τους στην ενισχυση μοναδικου εκκινητη CY1109828T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32345501P 2001-09-19 2001-09-19
EP02775901A EP1436404B1 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification

Publications (1)

Publication Number Publication Date
CY1109828T1 true CY1109828T1 (el) 2014-09-10

Family

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Country Status (12)

Country Link
US (1) US7306906B2 (el)
EP (1) EP1436404B1 (el)
JP (2) JP4439262B2 (el)
AT (1) ATE448320T1 (el)
AU (1) AU2002341752B2 (el)
CA (1) CA2460639C (el)
CY (1) CY1109828T1 (el)
DE (1) DE60234369D1 (el)
DK (1) DK1436404T3 (el)
ES (1) ES2336433T3 (el)
PT (1) PT1436404E (el)
WO (1) WO2003025202A2 (el)

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JP5107274B2 (ja) 2012-12-26
WO2003025202A2 (en) 2003-03-27
EP1436404A4 (en) 2006-07-05
JP2009089716A (ja) 2009-04-30
EP1436404B1 (en) 2009-11-11
CA2460639A1 (en) 2003-03-27
WO2003025202A3 (en) 2004-02-12
DK1436404T3 (da) 2010-03-08
US7306906B2 (en) 2007-12-11
CA2460639C (en) 2013-07-16
PT1436404E (pt) 2009-12-16
JP2005503802A (ja) 2005-02-10
JP4439262B2 (ja) 2010-03-24
DE60234369D1 (de) 2009-12-24
ATE448320T1 (de) 2009-11-15
AU2002341752B2 (en) 2008-04-03
US20040072164A1 (en) 2004-04-15
ES2336433T3 (es) 2010-04-13
EP1436404A2 (en) 2004-07-14

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