DK1436404T3 - Manipulerede templates og deres anvendelse i single-primer amplifikation - Google Patents

Manipulerede templates og deres anvendelse i single-primer amplifikation

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Publication number
DK1436404T3
DK1436404T3 DK02775901.8T DK02775901T DK1436404T3 DK 1436404 T3 DK1436404 T3 DK 1436404T3 DK 02775901 T DK02775901 T DK 02775901T DK 1436404 T3 DK1436404 T3 DK 1436404T3
Authority
DK
Denmark
Prior art keywords
primer
polynucleotide
template
anneals
predetermined sequence
Prior art date
Application number
DK02775901.8T
Other languages
English (en)
Inventor
Toshiaki Maruyama
Ying-Chi Lin
Mark Renshaw
Shana Frederickson
Katherine S Bowdish
Original Assignee
Alexion Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc filed Critical Alexion Pharma Inc
Application granted granted Critical
Publication of DK1436404T3 publication Critical patent/DK1436404T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
DK02775901.8T 2001-09-19 2002-09-19 Manipulerede templates og deres anvendelse i single-primer amplifikation DK1436404T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32345501P 2001-09-19 2001-09-19
PCT/US2002/029889 WO2003025202A2 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification

Publications (1)

Publication Number Publication Date
DK1436404T3 true DK1436404T3 (da) 2010-03-08

Family

ID=23259271

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02775901.8T DK1436404T3 (da) 2001-09-19 2002-09-19 Manipulerede templates og deres anvendelse i single-primer amplifikation

Country Status (12)

Country Link
US (1) US7306906B2 (da)
EP (1) EP1436404B1 (da)
JP (2) JP4439262B2 (da)
AT (1) ATE448320T1 (da)
AU (1) AU2002341752B2 (da)
CA (1) CA2460639C (da)
CY (1) CY1109828T1 (da)
DE (1) DE60234369D1 (da)
DK (1) DK1436404T3 (da)
ES (1) ES2336433T3 (da)
PT (1) PT1436404E (da)
WO (1) WO2003025202A2 (da)

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Also Published As

Publication number Publication date
JP5107274B2 (ja) 2012-12-26
EP1436404A4 (en) 2006-07-05
JP4439262B2 (ja) 2010-03-24
EP1436404B1 (en) 2009-11-11
JP2009089716A (ja) 2009-04-30
ES2336433T3 (es) 2010-04-13
CA2460639A1 (en) 2003-03-27
JP2005503802A (ja) 2005-02-10
CA2460639C (en) 2013-07-16
CY1109828T1 (el) 2014-09-10
US7306906B2 (en) 2007-12-11
ATE448320T1 (de) 2009-11-15
AU2002341752B2 (en) 2008-04-03
EP1436404A2 (en) 2004-07-14
US20040072164A1 (en) 2004-04-15
WO2003025202A3 (en) 2004-02-12
PT1436404E (pt) 2009-12-16
WO2003025202A2 (en) 2003-03-27
DE60234369D1 (de) 2009-12-24

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