ATE540127T1 - Multiplex pcr - Google Patents

Multiplex pcr

Info

Publication number
ATE540127T1
ATE540127T1 AT02803689T AT02803689T ATE540127T1 AT E540127 T1 ATE540127 T1 AT E540127T1 AT 02803689 T AT02803689 T AT 02803689T AT 02803689 T AT02803689 T AT 02803689T AT E540127 T1 ATE540127 T1 AT E540127T1
Authority
AT
Austria
Prior art keywords
templates
multiplex pcr
appending
distinct sequence
nucleic acid
Prior art date
Application number
AT02803689T
Other languages
English (en)
Inventor
Malek Faham
Eugeni Anatolyevich Namsaraev
Thomas Willis
Original Assignee
Affymetrix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affymetrix Inc filed Critical Affymetrix Inc
Application granted granted Critical
Publication of ATE540127T1 publication Critical patent/ATE540127T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02803689T 2001-11-19 2002-11-19 Multiplex pcr ATE540127T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33169301P 2001-11-19 2001-11-19
PCT/US2002/037238 WO2003044177A2 (en) 2001-11-19 2002-11-19 Multiplex pcr

Publications (1)

Publication Number Publication Date
ATE540127T1 true ATE540127T1 (de) 2012-01-15

Family

ID=23294970

Family Applications (2)

Application Number Title Priority Date Filing Date
AT02803690T ATE425263T1 (de) 2001-11-19 2002-11-19 Multiplex-oligonukleotidaddition und zielamplifikation
AT02803689T ATE540127T1 (de) 2001-11-19 2002-11-19 Multiplex pcr

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT02803690T ATE425263T1 (de) 2001-11-19 2002-11-19 Multiplex-oligonukleotidaddition und zielamplifikation

Country Status (9)

Country Link
US (3) US7510829B2 (de)
EP (2) EP1456416B1 (de)
JP (2) JP2005509440A (de)
CN (2) CN1697882A (de)
AT (2) ATE425263T1 (de)
AU (2) AU2002366098A1 (de)
CA (2) CA2467460A1 (de)
DE (1) DE60231543D1 (de)
WO (2) WO2003044216A2 (de)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467460A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
WO2004029223A2 (en) * 2002-09-30 2004-04-08 Parallele Bioscience, Inc. Polynucleotide synthesis and labeling by kinetic sampling ligation
US20060141498A1 (en) * 2003-09-25 2006-06-29 Affymetrix, Inc. Methods for fragmenting nucleic acid
EP1687437A4 (de) * 2003-10-13 2008-01-02 Genaco Biomedial Products Inc Verfahren und kit zur auf primern beruhenden amplifikation von nukleinsäuren
US8029454B2 (en) * 2003-11-05 2011-10-04 Baxter International Inc. High convection home hemodialysis/hemofiltration and sorbent system
US7531306B2 (en) 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US20060029954A1 (en) * 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
EP3257949A1 (de) 2005-06-15 2017-12-20 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige mischungen von nichtüberlappenden fragmenten
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP2029779A4 (de) 2006-06-14 2010-01-20 Living Microsystems Inc Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
CN100427607C (zh) * 2006-09-19 2008-10-22 南京师范大学 一种克隆新基因的试剂盒及其应用方法
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
EP2195452B1 (de) 2007-08-29 2012-03-14 Sequenom, Inc. Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US8114276B2 (en) 2007-10-24 2012-02-14 Baxter International Inc. Personal hemodialysis system
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
EP2285974A2 (de) 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen
EP2408936A4 (de) * 2009-03-18 2013-01-30 Sequenom Inc Verwendung wärmestabiler endonukleasen zur herstellung von reportermolekülen
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
CN102791877A (zh) * 2009-11-06 2012-11-21 酶学有限公司 使用双链核酸复合物与耐热聚合酶用于合成脱氧核糖核苷酸链的组合物和方法
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
CN101956023B (zh) * 2010-09-16 2012-10-31 西南大学 检测十种主要柑桔病害病原的基因芯片
CN102399864A (zh) * 2010-09-16 2012-04-04 上海迦美生物科技有限公司 基于引物保护解除的甲基化dna的检测方法
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN103562407A (zh) 2011-02-09 2014-02-05 伯乐生命医学产品有限公司 核酸的分析
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
GB2492042B (en) * 2011-05-11 2020-04-15 Agilent Technologies Inc Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
EP2802666B1 (de) 2012-01-13 2018-09-19 Data2Bio Genotypisierung durch eine sequenzierung der nächsten generation
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US9139870B2 (en) * 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
EP3005200A2 (de) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Verfahren und systeme zur speicherung von sequenzlesedaten
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
EP3024948B1 (de) 2013-07-25 2020-01-15 Bio-rad Laboratories, Inc. Genetische tests zur bestimmung einer viralen rekombinationsfrequenz
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
EP3058096A1 (de) 2013-10-18 2016-08-24 Good Start Genetics, Inc. Verfahren zur beurteilung eines genomischen bereichs einer person
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
EP2983297A1 (de) * 2014-08-08 2016-02-10 Thomson Licensing Codeerzeugungsverfahren, Codeerzeugungsvorrichtung und computerlesbares Speichermedium
US10174383B2 (en) * 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
CN107002123A (zh) * 2014-08-14 2017-08-01 生命技术公司 多重转录组分析
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
EP3224595A4 (de) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Prozesssteuerung für erhöhte robustheit von genetischen assays
US9441268B2 (en) * 2014-11-26 2016-09-13 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapping hydrolysis probes
WO2016112073A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
CN108026580A (zh) * 2015-03-19 2018-05-11 康代思锐公司 用于核酸检测的被编码的双链探针及其用途
RU2753883C2 (ru) 2015-09-18 2021-08-24 Ванадис Дайэгностикс Набор зондов для анализа образцов днк и способы их использования
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
WO2018183607A1 (en) 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
US11441174B2 (en) 2017-06-02 2022-09-13 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
CN110914448A (zh) 2017-06-02 2020-03-24 昂飞股份有限公司 使用差异性标记的等位基因特异性探针分析混合样品的基于阵列的方法
CN107385052B (zh) * 2017-08-08 2020-10-30 中国林业科学研究院热带林业研究所 用于鉴定桉树无性系的str引物及其应用
CN111918965A (zh) * 2018-04-28 2020-11-10 深圳华大生命科学研究院 一种胎儿游离核酸的富集方法及其应用
CN108660146B (zh) * 2018-05-22 2019-03-19 南京诺唯赞生物科技有限公司 一种提高PCR片段兼容性与效率的Topo1连接方法
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
US12522856B2 (en) 2018-09-11 2026-01-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for multiplex gene amplification from ultra-low DNA input amounts and uses thereof
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US588731A (en) * 1897-08-24 Slate-sprinkler
TW205070B (de) * 1986-06-30 1993-05-01 Takeda Pharm Industry Co Ltd
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA1339731C (en) 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5439793A (en) 1990-07-19 1995-08-08 Syntex (U.S.A.) Inc. Method for producing a polynucleotide having an intramolecularly base-paired structure
CA2119557C (en) * 1991-09-24 2004-02-24 Marc Zabeau Selective restriction fragment amplification: a general method for dna fingerprinting
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5851770A (en) 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
WO1996006190A2 (en) 1994-08-19 1996-02-29 Perkin-Elmer Corporation Coupled amplification and ligation method
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5728526A (en) 1995-06-07 1998-03-17 Oncor, Inc. Method for analyzing a nucleotide sequence
NZ298494A (en) 1995-07-11 1999-09-29 Forfas Trading As Bioresearch Glycosylase mediated detection of nucleotide sequences at candidate loci
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6355422B1 (en) 1998-06-24 2002-03-12 City Of Hope Single tube PCR assay for detection of chromosomal mutations: application to the inversion hotspot in the factor VIII gene including optional use of subcycling PCR
DE1020534T1 (de) 1998-11-09 2001-03-01 Eiken Kagaku K.K., Tokio/Tokyo Verfahren zur synthetisieren von nukleinsäure
US20020119448A1 (en) 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
AU6345700A (en) * 1999-07-14 2001-02-05 Packard Bioscience Company Derivative nucleic acids and uses thereof
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
GB9927576D0 (en) 1999-11-23 2000-01-19 Univ Birmingham Novel assay
US6207424B1 (en) 1999-11-23 2001-03-27 Maxim Biotech, Inc. Self-primed amplification system
ATE411397T1 (de) * 2000-02-07 2008-10-15 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
WO2001094625A2 (en) 2000-06-06 2001-12-13 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
WO2002029112A1 (en) 2000-10-04 2002-04-11 The Board Of Trustees Of The Leland Stanford Junior University Renaturation, reassociation, association and hybridization of nucleic acid molecules
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
JP4588976B2 (ja) 2001-01-25 2010-12-01 ルミネックス モレキュラー ダイアグノスティックス， インク． タグおよびタグ相補体としてのポリヌクレオチド、製品、ならびにその使用法
CA2439402A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
CA2467460A1 (en) 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification

Also Published As

Publication number Publication date
EP1456416A4 (de) 2006-01-11
WO2003044177A3 (en) 2003-08-28
CN100396788C (zh) 2008-06-25
JP2005509433A (ja) 2005-04-14
US20030096291A1 (en) 2003-05-22
US20030104459A1 (en) 2003-06-05
EP1453979A2 (de) 2004-09-08
DE60231543D1 (de) 2009-04-23
EP1453979A4 (de) 2005-12-28
AU2002366099A1 (en) 2003-06-10
WO2003044177A2 (en) 2003-05-30
CN1610758A (zh) 2005-04-27
CA2467460A1 (en) 2003-05-30
US7754451B2 (en) 2010-07-13
CN1697882A (zh) 2005-11-16
EP1456416B1 (de) 2009-03-11
ATE425263T1 (de) 2009-03-15
WO2003044216A2 (en) 2003-05-30
US7510829B2 (en) 2009-03-31
AU2002366098A1 (en) 2003-06-10
EP1456416A2 (de) 2004-09-15
EP1453979B1 (de) 2012-01-04
US7208295B2 (en) 2007-04-24
US20090004701A1 (en) 2009-01-01
JP2005509440A (ja) 2005-04-14
CA2467577A1 (en) 2003-05-30
WO2003044216A3 (en) 2003-08-14

Similar Documents

Publication Publication Date Title
ATE540127T1 (de) Multiplex pcr
DK1527176T3 (da) Yderligere nye former af interfererende RNA-molekyler
WO2004042029A3 (en) Oligomers comprising modified bases for binding cytosine and uracil or thymine and their use
DK1196637T3 (da) Peptidacceptor-ligeringsmetoder
CY1109828T1 (el) Κατασκευασμενα προτυπα και η χρηση τους στην ενισχυση μοναδικου εκκινητη
DK1411122T3 (da) Fremgangsmåde til fremstilling af nukleinsyremolekyler
NO20070263L (no) Immunostimulerende oligonukleotidmultimerer
DK1072010T3 (da) Oligonukleotidmedieret nukleinsyrerekombination
WO2005000201A3 (en) Modulation of apolipoprotein (a) expression
ATE335852T1 (de) Happier mapping
WO2005120230A3 (en) POSITIONALLY MODIFIED siRNA CONSTRUCTS
DK0973940T3 (da) En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek
AR030961A1 (es) Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas
EP1641936A4 (de) Verfahren zur genomamplifikation
ATE518948T1 (de) Verbesserte polymerasen
DE602005022443D1 (de) Schnelle synthese von oligonukleotiden
FR2792000B1 (fr) Procede de cartographie d'une molecule d'adn comprenant une etape d'amplification ad infinitum
ATE223499T1 (de) Von kreisförmiger matrize abhängige kettenreaktion
DE60123334D1 (de) Methode für die Produktion von Nukleotiden durch Fermentierung
ATE340873T1 (de) Verfahren zur durchführung einer subtraktiven hybridisierung
EP1570082A4 (de) Modulation der notch2-expression
NO20052073D0 (no) ACC-gen.
DE602007013084D1 (de) Neues markiertes oligonukleotid
IT1318702B1 (it) Metodo per la determinazione quantitativa del gene smn1.