ATE540127T1 - Multiplex pcr - Google Patents

Multiplex pcr

Info

Publication number
ATE540127T1
ATE540127T1 AT02803689T AT02803689T ATE540127T1 AT E540127 T1 ATE540127 T1 AT E540127T1 AT 02803689 T AT02803689 T AT 02803689T AT 02803689 T AT02803689 T AT 02803689T AT E540127 T1 ATE540127 T1 AT E540127T1
Authority
AT
Austria
Prior art keywords
templates
multiplex pcr
appending
distinct sequence
nucleic acid
Prior art date
Application number
AT02803689T
Other languages
English (en)
Inventor
Malek Faham
Eugeni Anatolyevich Namsaraev
Thomas Willis
Original Assignee
Affymetrix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affymetrix Inc filed Critical Affymetrix Inc
Application granted granted Critical
Publication of ATE540127T1 publication Critical patent/ATE540127T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02803689T 2001-11-19 2002-11-19 Multiplex pcr ATE540127T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33169301P 2001-11-19 2001-11-19
PCT/US2002/037238 WO2003044177A2 (en) 2001-11-19 2002-11-19 Multiplex pcr

Publications (1)

Publication Number Publication Date
ATE540127T1 true ATE540127T1 (de) 2012-01-15

Family

ID=23294970

Family Applications (2)

Application Number Title Priority Date Filing Date
AT02803689T ATE540127T1 (de) 2001-11-19 2002-11-19 Multiplex pcr
AT02803690T ATE425263T1 (de) 2001-11-19 2002-11-19 Multiplex-oligonukleotidaddition und zielamplifikation

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT02803690T ATE425263T1 (de) 2001-11-19 2002-11-19 Multiplex-oligonukleotidaddition und zielamplifikation

Country Status (9)

Country Link
US (3) US7510829B2 (de)
EP (2) EP1456416B1 (de)
JP (2) JP2005509433A (de)
CN (2) CN100396788C (de)
AT (2) ATE540127T1 (de)
AU (2) AU2002366098A1 (de)
CA (2) CA2467577A1 (de)
DE (1) DE60231543D1 (de)
WO (2) WO2003044177A2 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60231543D1 (de) * 2001-11-19 2009-04-23 Parallele Bioscience Inc Multiplex-oligonukleotidaddition und zielamplifikation
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
JP2006500959A (ja) * 2002-09-30 2006-01-12 パラレル バイオサイエンス， インコーポレイテッド 動的サンプリング結合によるポリヌクレオチド合成および標識化
US20060141498A1 (en) * 2003-09-25 2006-06-29 Affymetrix, Inc. Methods for fragmenting nucleic acid
NZ546903A (en) * 2003-10-13 2009-06-26 Genaco Biomedical Products Inc Method and kit for primer based amplification of nucleic acids
US8029454B2 (en) 2003-11-05 2011-10-04 Baxter International Inc. High convection home hemodialysis/hemofiltration and sorbent system
US7531306B2 (en) 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US20060029954A1 (en) * 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
DK2620510T4 (da) 2005-06-15 2020-03-30 Complete Genomics Inc Enkeltmolekyle-arrays til genetisk og kemisk analyse
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
EP2589668A1 (de) 2006-06-14 2013-05-08 Verinata Health, Inc Analyse seltener Zellen mittels Probentrennung und DNA-Etiketten
CN100427607C (zh) * 2006-09-19 2008-10-22 南京师范大学 一种克隆新基因的试剂盒及其应用方法
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
EP2195452B1 (de) 2007-08-29 2012-03-14 Sequenom, Inc. Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US8114276B2 (en) 2007-10-24 2012-02-14 Baxter International Inc. Personal hemodialysis system
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
US9068209B2 (en) 2008-05-14 2015-06-30 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
JP2012525147A (ja) 2009-04-30 2012-10-22 グッド スタート ジェネティクス， インコーポレイテッド 遺伝マーカーを評価するための方法および組成物
US20110111462A1 (en) * 2009-11-06 2011-05-12 Stephen Picone Composition and Method for Synthesizing a Deoxyribonucleotide Chain Using a Double Stranded Nucleic Acid Complex with a Thermostable Polymerase
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
CN102399864A (zh) * 2010-09-16 2012-04-04 上海迦美生物科技有限公司 基于引物保护解除的甲基化dna的检测方法
CN101956023B (zh) * 2010-09-16 2012-10-31 西南大学 检测十种主要柑桔病害病原的基因芯片
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2673380B1 (de) 2011-02-09 2018-12-12 Bio-Rad Laboratories, Inc. Analyse von nukleinsäuren
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
GB2492042B (en) * 2011-05-11 2020-04-15 Agilent Technologies Inc Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region
EP2768983A4 (de) 2011-10-17 2015-06-03 Good Start Genetics Inc Analyseverfahren
EP3434789A1 (de) 2012-01-13 2019-01-30 Data2Bio Genotypisierung durch eine sequenzierung der nächsten generation
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US9139870B2 (en) * 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
EP3005200A2 (de) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Verfahren und systeme zur speicherung von sequenzlesedaten
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN105555972B (zh) 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
EP2983297A1 (de) * 2014-08-08 2016-02-10 Thomson Licensing Codeerzeugungsverfahren, Codeerzeugungsvorrichtung und computerlesbares Speichermedium
US10174383B2 (en) 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
EP3180445A1 (de) * 2014-08-14 2017-06-21 Life Technologies Corporation Multiplex-transkriptomanalyse
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
WO2016048829A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US9441268B2 (en) * 2014-11-26 2016-09-13 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapping hydrolysis probes
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
CN114196732A (zh) * 2015-03-19 2022-03-18 康代思锐公司 用于核酸检测的被编码的双链探针及其用途
PL3350342T3 (pl) 2015-09-18 2020-08-24 Vanadis Diagnostics Zestaw sond do analizowania próbki DNA i sposób jego zastosowania
CA3041645C (en) 2016-10-24 2021-11-02 Geneinfosec, Inc. Concealing information present within nucleic acids
CA3056650A1 (en) 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
US11441174B2 (en) 2017-06-02 2022-09-13 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
US11603557B2 (en) 2017-06-02 2023-03-14 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
CN107385052B (zh) * 2017-08-08 2020-10-30 中国林业科学研究院热带林业研究所 用于鉴定桉树无性系的str引物及其应用
CN111918965A (zh) * 2018-04-28 2020-11-10 深圳华大生命科学研究院 一种胎儿游离核酸的富集方法及其应用
CN108660146B (zh) * 2018-05-22 2019-03-19 南京诺唯赞生物科技有限公司 一种提高PCR片段兼容性与效率的Topo1连接方法
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US588731A (en) * 1897-08-24 Slate-sprinkler
TW205070B (de) * 1986-06-30 1993-05-01 Takeda Pharm Industry Co Ltd
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5439793A (en) 1990-07-19 1995-08-08 Syntex (U.S.A.) Inc. Method for producing a polynucleotide having an intramolecularly base-paired structure
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
RU2182176C2 (ru) 1991-09-24 2002-05-10 Кейгене Н.В. Способ селективной амплификации, олигонуклеотид и набор для селективной амплификации
US5612199A (en) 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5851770A (en) * 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
CA2195562A1 (en) 1994-08-19 1996-02-29 Pe Corporation (Ny) Coupled amplification and ligation method
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5728526A (en) 1995-06-07 1998-03-17 Oncor, Inc. Method for analyzing a nucleotide sequence
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
WO1997003210A1 (en) * 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
EP0912761A4 (de) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6355422B1 (en) * 1998-06-24 2002-03-12 City Of Hope Single tube PCR assay for detection of chromosomal mutations: application to the inversion hotspot in the factor VIII gene including optional use of subcycling PCR
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
DE69940623D1 (de) * 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
US20020119448A1 (en) 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
ATE409236T1 (de) * 1999-07-14 2008-10-15 Packard Bioscience Company Derivatisierte nukleinsäuren und deren verwendung
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
GB9927576D0 (en) 1999-11-23 2000-01-19 Univ Birmingham Novel assay
US6207424B1 (en) 1999-11-23 2001-03-27 Maxim Biotech, Inc. Self-primed amplification system
ATE492652T1 (de) * 2000-02-07 2011-01-15 Illumina Inc Nukleinsäuredetektionsverfahren mit universellem priming
EP1313881A2 (de) 2000-06-06 2003-05-28 TM Bioscience Corporation Fangsonden für nuklensäuren sowie verwendung derselben
AU2002211520A1 (en) 2000-10-04 2002-04-15 The Board Of Trustees Of The Leland Stanford Junior University Renaturation, reassociation, association and hybridization of nucleic acid molecules
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
JP4422963B2 (ja) 2001-01-25 2010-03-03 ルミネックス モレキュラー ダイアグノスティックス， インク． 標識および標識補体として使用するための非クロス交雑しているポリヌクレオチドのファミリー、製品、ならびにその使用方法
CA2439402A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
DE60231543D1 (de) 2001-11-19 2009-04-23 Parallele Bioscience Inc Multiplex-oligonukleotidaddition und zielamplifikation

Also Published As

Publication number Publication date
CN1610758A (zh) 2005-04-27
AU2002366099A1 (en) 2003-06-10
US20090004701A1 (en) 2009-01-01
EP1453979A4 (de) 2005-12-28
JP2005509440A (ja) 2005-04-14
US7510829B2 (en) 2009-03-31
ATE425263T1 (de) 2009-03-15
US20030096291A1 (en) 2003-05-22
CN1697882A (zh) 2005-11-16
EP1453979A2 (de) 2004-09-08
DE60231543D1 (de) 2009-04-23
JP2005509433A (ja) 2005-04-14
EP1456416A4 (de) 2006-01-11
EP1456416A2 (de) 2004-09-15
CA2467460A1 (en) 2003-05-30
CA2467577A1 (en) 2003-05-30
EP1453979B1 (de) 2012-01-04
WO2003044177A3 (en) 2003-08-28
US20030104459A1 (en) 2003-06-05
CN100396788C (zh) 2008-06-25
WO2003044177A2 (en) 2003-05-30
AU2002366098A1 (en) 2003-06-10
US7754451B2 (en) 2010-07-13
US7208295B2 (en) 2007-04-24
WO2003044216A2 (en) 2003-05-30
EP1456416B1 (de) 2009-03-11
WO2003044216A3 (en) 2003-08-14

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