ATE540127T1 - Multiplex pcr - Google Patents
Multiplex pcrInfo
- Publication number
- ATE540127T1 ATE540127T1 AT02803689T AT02803689T ATE540127T1 AT E540127 T1 ATE540127 T1 AT E540127T1 AT 02803689 T AT02803689 T AT 02803689T AT 02803689 T AT02803689 T AT 02803689T AT E540127 T1 ATE540127 T1 AT E540127T1
- Authority
- AT
- Austria
- Prior art keywords
- templates
- multiplex pcr
- appending
- distinct sequence
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33169301P | 2001-11-19 | 2001-11-19 | |
PCT/US2002/037238 WO2003044177A2 (en) | 2001-11-19 | 2002-11-19 | Multiplex pcr |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE540127T1 true ATE540127T1 (de) | 2012-01-15 |
Family
ID=23294970
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02803690T ATE425263T1 (de) | 2001-11-19 | 2002-11-19 | Multiplex-oligonukleotidaddition und zielamplifikation |
AT02803689T ATE540127T1 (de) | 2001-11-19 | 2002-11-19 | Multiplex pcr |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02803690T ATE425263T1 (de) | 2001-11-19 | 2002-11-19 | Multiplex-oligonukleotidaddition und zielamplifikation |
Country Status (9)
Country | Link |
---|---|
US (3) | US7208295B2 (de) |
EP (2) | EP1456416B1 (de) |
JP (2) | JP2005509433A (de) |
CN (2) | CN100396788C (de) |
AT (2) | ATE425263T1 (de) |
AU (2) | AU2002366098A1 (de) |
CA (2) | CA2467577A1 (de) |
DE (1) | DE60231543D1 (de) |
WO (2) | WO2003044216A2 (de) |
Families Citing this family (67)
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US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
ATE425263T1 (de) * | 2001-11-19 | 2009-03-15 | Parallele Bioscience Inc | Multiplex-oligonukleotidaddition und zielamplifikation |
WO2004029223A2 (en) * | 2002-09-30 | 2004-04-08 | Parallele Bioscience, Inc. | Polynucleotide synthesis and labeling by kinetic sampling ligation |
US20060141498A1 (en) * | 2003-09-25 | 2006-06-29 | Affymetrix, Inc. | Methods for fragmenting nucleic acid |
KR100830623B1 (ko) * | 2003-10-13 | 2008-05-20 | 제나코 바이오메디컬 프로덕츠, 인코포레이티드 | 핵산의 프라이머 기초 멀티플렉스 증폭을 위한 방법 및 키트 |
US8029454B2 (en) | 2003-11-05 | 2011-10-04 | Baxter International Inc. | High convection home hemodialysis/hemofiltration and sorbent system |
EP1689879A4 (de) * | 2003-11-12 | 2008-08-27 | Geneohm Sciences Inc | Nukleinsäurehybridisierungsverfahren |
WO2006005081A2 (en) * | 2004-06-30 | 2006-01-12 | Applera Corporation | Compositions and methods for identifying nucleotides in polynucleotide sequences |
US7709197B2 (en) | 2005-06-15 | 2010-05-04 | Callida Genomics, Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
US8372584B2 (en) | 2006-06-14 | 2013-02-12 | The General Hospital Corporation | Rare cell analysis using sample splitting and DNA tags |
WO2007147074A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Use of highly parallel snp genotyping for fetal diagnosis |
US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
CN100427607C (zh) * | 2006-09-19 | 2008-10-22 | 南京师范大学 | 一种克隆新基因的试剂盒及其应用方法 |
US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
US7862999B2 (en) | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
US9404150B2 (en) | 2007-08-29 | 2016-08-02 | Sequenom, Inc. | Methods and compositions for universal size-specific PCR |
US9388457B2 (en) | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
US8114276B2 (en) | 2007-10-24 | 2012-02-14 | Baxter International Inc. | Personal hemodialysis system |
US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
US20090233809A1 (en) * | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
CA2723242A1 (en) | 2008-05-14 | 2009-11-19 | British Columbia Cancer Agency Branch | Gene synthesis by convergent assembly of oligonucleotide subsets |
WO2010107946A2 (en) * | 2009-03-18 | 2010-09-23 | Sequenom, Inc. | Use of thermostable endonucleases for generating reporter molecules |
AU2010242073C1 (en) | 2009-04-30 | 2015-12-24 | Good Start Genetics, Inc. | Methods and compositions for evaluating genetic markers |
CA2779689A1 (en) * | 2009-11-06 | 2011-05-12 | Enzymatics, Inc. | Composition and method for synthesizing a deoxyribonucleotide chain using a double stranded nucleic acid complex with a thermostable polymerase |
EP2504448B1 (de) * | 2009-11-25 | 2016-10-19 | Bio-Rad Laboratories, Inc. | Verfahren und zusammensetzung zur erkennung von genetischem material |
CN102399864A (zh) * | 2010-09-16 | 2012-04-04 | 上海迦美生物科技有限公司 | 基于引物保护解除的甲基化dna的检测方法 |
CN101956023B (zh) * | 2010-09-16 | 2012-10-31 | 西南大学 | 检测十种主要柑桔病害病原的基因芯片 |
US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
EP3940084A1 (de) | 2011-02-09 | 2022-01-19 | Bio-Rad Laboratories, Inc. | Analyse von nukleinsäuren |
US8759036B2 (en) | 2011-03-21 | 2014-06-24 | Affymetrix, Inc. | Methods for synthesizing pools of probes |
GB2492042B (en) * | 2011-05-11 | 2020-04-15 | Agilent Technologies Inc | Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region |
CA2852665A1 (en) | 2011-10-17 | 2013-04-25 | Good Start Genetics, Inc. | Analysis methods |
CN104334739A (zh) | 2012-01-13 | 2015-02-04 | Data生物有限公司 | 通过新一代测序进行基因分型 |
US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
AU2013308647B2 (en) * | 2012-08-30 | 2018-10-18 | Gen-Probe Incorporated | Multiphase nucleic acid amplification |
US8778609B1 (en) | 2013-03-14 | 2014-07-15 | Good Start Genetics, Inc. | Methods for analyzing nucleic acids |
EP3005200A2 (de) | 2013-06-03 | 2016-04-13 | Good Start Genetics, Inc. | Verfahren und systeme zur speicherung von sequenzlesedaten |
US9708657B2 (en) | 2013-07-01 | 2017-07-18 | Adaptive Biotechnologies Corp. | Method for generating clonotype profiles using sequence tags |
WO2015013681A1 (en) | 2013-07-25 | 2015-01-29 | Bio-Rad Laboratories, Inc. | Genetic assays |
EP3058096A1 (de) | 2013-10-18 | 2016-08-24 | Good Start Genetics, Inc. | Verfahren zur beurteilung eines genomischen bereichs einer person |
US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
GB2520765A (en) | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Multiplex detection of nucleic acids |
GB2520763A (en) | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Nucleic acid probe and method of detecting genomic fragments |
US11053548B2 (en) | 2014-05-12 | 2021-07-06 | Good Start Genetics, Inc. | Methods for detecting aneuploidy |
EP2983297A1 (de) * | 2014-08-08 | 2016-02-10 | Thomson Licensing | Codeerzeugungsverfahren, Codeerzeugungsvorrichtung und computerlesbares Speichermedium |
US10174383B2 (en) * | 2014-08-13 | 2019-01-08 | Vanadis Diagnostics | Method of estimating the amount of a methylated locus in a sample |
CN107002123A (zh) * | 2014-08-14 | 2017-08-01 | 生命技术公司 | 多重转录组分析 |
US11408024B2 (en) | 2014-09-10 | 2022-08-09 | Molecular Loop Biosciences, Inc. | Methods for selectively suppressing non-target sequences |
JP2017536087A (ja) | 2014-09-24 | 2017-12-07 | グッド スタート ジェネティクス, インコーポレイテッド | 遺伝子アッセイのロバストネスを増大させるためのプロセス制御 |
US9441268B2 (en) * | 2014-11-26 | 2016-09-13 | Roche Molecular Systems, Inc. | Detecting single nucleotide polymorphism using overlapping hydrolysis probes |
CA3010579A1 (en) | 2015-01-06 | 2016-07-14 | Good Start Genetics, Inc. | Screening for structural variants |
CN108026580A (zh) * | 2015-03-19 | 2018-05-11 | 康代思锐公司 | 用于核酸检测的被编码的双链探针及其用途 |
EP3350342B1 (de) | 2015-09-18 | 2020-02-19 | Vanadis Diagnostics | Sondensatz zur analyse einer dna-probe und verfahren zur verwendung davon |
US11667951B2 (en) | 2016-10-24 | 2023-06-06 | Geneinfosec, Inc. | Concealing information present within nucleic acids |
EP3601610A1 (de) | 2017-03-30 | 2020-02-05 | Pioneer Hi-Bred International, Inc. | Verfahren zur identifizierung und charakterisierung von geneditierungsvariationen in nukleinsäuren |
US11441174B2 (en) | 2017-06-02 | 2022-09-13 | Affymetrix, Inc. | Array-based methods for analysing mixed samples using differently labelled allele-specific probes |
WO2018223055A1 (en) | 2017-06-02 | 2018-12-06 | Affymetrix, Inc. | Array-based methods for analysing mixed samples using differently labelled allele-specific probes |
CN107385052B (zh) * | 2017-08-08 | 2020-10-30 | 中国林业科学研究院热带林业研究所 | 用于鉴定桉树无性系的str引物及其应用 |
WO2019205132A1 (zh) * | 2018-04-28 | 2019-10-31 | 深圳华大生命科学研究院 | 一种胎儿游离核酸的富集方法及其应用 |
CN108660146B (zh) * | 2018-05-22 | 2019-03-19 | 南京诺唯赞生物科技有限公司 | 一种提高PCR片段兼容性与效率的Topo1连接方法 |
SG11202101934SA (en) | 2018-07-30 | 2021-03-30 | Readcoor Llc | Methods and systems for sample processing or analysis |
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US588731A (en) * | 1897-08-24 | Slate-sprinkler | ||
TW205070B (de) | 1986-06-30 | 1993-05-01 | Takeda Pharm Industry Co Ltd | |
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GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
US5595891A (en) * | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
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JP3236295B2 (ja) | 1991-09-24 | 2001-12-10 | ケイヘーネ・エヌ・ベー | 選択的な制限断片増幅:一般的なdnaフィンガプリント法 |
US5612199A (en) * | 1991-10-11 | 1997-03-18 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
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IE20000887A1 (en) | 2000-11-03 | 2002-12-11 | Univ College Cork Nat Univ Ie | Method for the amplification and optional characterisation of nucleic acids |
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CA2439402A1 (en) | 2001-03-02 | 2002-09-12 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Pcr method |
ATE425263T1 (de) * | 2001-11-19 | 2009-03-15 | Parallele Bioscience Inc | Multiplex-oligonukleotidaddition und zielamplifikation |
-
2002
- 2002-11-19 AT AT02803690T patent/ATE425263T1/de not_active IP Right Cessation
- 2002-11-19 AU AU2002366098A patent/AU2002366098A1/en not_active Abandoned
- 2002-11-19 EP EP02803690A patent/EP1456416B1/de not_active Expired - Lifetime
- 2002-11-19 JP JP2003545802A patent/JP2005509433A/ja active Pending
- 2002-11-19 WO PCT/US2002/037239 patent/WO2003044216A2/en active Application Filing
- 2002-11-19 EP EP02803689A patent/EP1453979B1/de not_active Expired - Lifetime
- 2002-11-19 CA CA002467577A patent/CA2467577A1/en not_active Abandoned
- 2002-11-19 JP JP2003545837A patent/JP2005509440A/ja active Pending
- 2002-11-19 CN CNB028265912A patent/CN100396788C/zh not_active Expired - Fee Related
- 2002-11-19 DE DE60231543T patent/DE60231543D1/de not_active Expired - Fee Related
- 2002-11-19 WO PCT/US2002/037238 patent/WO2003044177A2/en active Application Filing
- 2002-11-19 CA CA002467460A patent/CA2467460A1/en not_active Abandoned
- 2002-11-19 AT AT02803689T patent/ATE540127T1/de active
- 2002-11-19 US US10/300,311 patent/US7208295B2/en not_active Expired - Lifetime
- 2002-11-19 AU AU2002366099A patent/AU2002366099A1/en not_active Abandoned
- 2002-11-19 CN CNA028265955A patent/CN1697882A/zh active Pending
- 2002-11-19 US US10/300,285 patent/US7510829B2/en not_active Expired - Fee Related
-
2007
- 2007-04-24 US US11/739,654 patent/US7754451B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20030096291A1 (en) | 2003-05-22 |
EP1453979A4 (de) | 2005-12-28 |
JP2005509433A (ja) | 2005-04-14 |
WO2003044177A3 (en) | 2003-08-28 |
WO2003044216A2 (en) | 2003-05-30 |
AU2002366098A1 (en) | 2003-06-10 |
AU2002366099A1 (en) | 2003-06-10 |
CN1610758A (zh) | 2005-04-27 |
CA2467577A1 (en) | 2003-05-30 |
WO2003044216A3 (en) | 2003-08-14 |
CA2467460A1 (en) | 2003-05-30 |
CN1697882A (zh) | 2005-11-16 |
EP1456416A2 (de) | 2004-09-15 |
CN100396788C (zh) | 2008-06-25 |
DE60231543D1 (de) | 2009-04-23 |
US7754451B2 (en) | 2010-07-13 |
EP1456416A4 (de) | 2006-01-11 |
EP1453979A2 (de) | 2004-09-08 |
JP2005509440A (ja) | 2005-04-14 |
WO2003044177A2 (en) | 2003-05-30 |
ATE425263T1 (de) | 2009-03-15 |
EP1456416B1 (de) | 2009-03-11 |
EP1453979B1 (de) | 2012-01-04 |
US20030104459A1 (en) | 2003-06-05 |
US7208295B2 (en) | 2007-04-24 |
US20090004701A1 (en) | 2009-01-01 |
US7510829B2 (en) | 2009-03-31 |
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