ATE448320T1 - Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation - Google Patents

Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation

Info

Publication number
ATE448320T1
ATE448320T1 AT02775901T AT02775901T ATE448320T1 AT E448320 T1 ATE448320 T1 AT E448320T1 AT 02775901 T AT02775901 T AT 02775901T AT 02775901 T AT02775901 T AT 02775901T AT E448320 T1 ATE448320 T1 AT E448320T1
Authority
AT
Austria
Prior art keywords
primer
polynucleotide
template
anneals
predetermined sequence
Prior art date
Application number
AT02775901T
Other languages
English (en)
Inventor
Katherine Bowdish
Shana Frederickson
Toshiaki Maruyama
Ying-Chi Lin
Mark Renshaw
Original Assignee
Alexion Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc filed Critical Alexion Pharma Inc
Application granted granted Critical
Publication of ATE448320T1 publication Critical patent/ATE448320T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
AT02775901T 2001-09-19 2002-09-19 Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation ATE448320T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32345501P 2001-09-19 2001-09-19
PCT/US2002/029889 WO2003025202A2 (en) 2001-09-19 2002-09-19 Engineered templates and their use in single primer amplification

Publications (1)

Publication Number Publication Date
ATE448320T1 true ATE448320T1 (de) 2009-11-15

Family

ID=23259271

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02775901T ATE448320T1 (de) 2001-09-19 2002-09-19 Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation

Country Status (12)

Country Link
US (1) US7306906B2 (de)
EP (1) EP1436404B1 (de)
JP (2) JP4439262B2 (de)
AT (1) ATE448320T1 (de)
AU (1) AU2002341752B2 (de)
CA (1) CA2460639C (de)
CY (1) CY1109828T1 (de)
DE (1) DE60234369D1 (de)
DK (1) DK1436404T3 (de)
ES (1) ES2336433T3 (de)
PT (1) PT1436404E (de)
WO (1) WO2003025202A2 (de)

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Also Published As

Publication number Publication date
JP5107274B2 (ja) 2012-12-26
WO2003025202A2 (en) 2003-03-27
EP1436404A4 (de) 2006-07-05
JP2009089716A (ja) 2009-04-30
EP1436404B1 (de) 2009-11-11
CA2460639A1 (en) 2003-03-27
WO2003025202A3 (en) 2004-02-12
DK1436404T3 (da) 2010-03-08
US7306906B2 (en) 2007-12-11
CA2460639C (en) 2013-07-16
PT1436404E (pt) 2009-12-16
CY1109828T1 (el) 2014-09-10
JP2005503802A (ja) 2005-02-10
JP4439262B2 (ja) 2010-03-24
DE60234369D1 (de) 2009-12-24
AU2002341752B2 (en) 2008-04-03
US20040072164A1 (en) 2004-04-15
ES2336433T3 (es) 2010-04-13
EP1436404A2 (de) 2004-07-14

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