NO20022171L - Fremgangsmåte for syntese av nukleinsyre - Google Patents
Fremgangsmåte for syntese av nukleinsyreInfo
- Publication number
- NO20022171L NO20022171L NO20022171A NO20022171A NO20022171L NO 20022171 L NO20022171 L NO 20022171L NO 20022171 A NO20022171 A NO 20022171A NO 20022171 A NO20022171 A NO 20022171A NO 20022171 L NO20022171 L NO 20022171L
- Authority
- NO
- Norway
- Prior art keywords
- nucleic acid
- synthesis
- oligonucleotide
- synthesizing
- primer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Abstract
Foreliggende oppfinnelse gjelder et oligonukleotid med en ny struktur og en fremgangsmåte for syntese av en nukleinsyre ved anvendelse av oligonukleotidet som primer. I sin 5'-ende er oligonukleotidet utstyrt med en basesekvens som i det vesentlige er identisk med området som skal syntetiseres ved anvendelse av primeren som utgangspunkt for syntesen. Således kan en nukleinsyre syntetiseres i en iso-term reaksjon ved anvendelse av en enkel reagensblanding. Videre tilveie-bringes en fremgangsmåte for syntese av en nukleinsyre med høy spesifisitet, basert på den ovenfor beskrevne fremgangsmåte for syntese av en nukleinsyre .
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP1999/006213 WO2000028082A1 (fr) | 1998-11-09 | 1999-11-08 | Procede de synthese d'acide nucleique |
PCT/JP2000/001919 WO2001034790A1 (fr) | 1999-11-08 | 2000-03-28 | Procede de synthese d'un acide nucleique |
Publications (3)
Publication Number | Publication Date |
---|---|
NO20022171D0 NO20022171D0 (no) | 2002-05-07 |
NO20022171L true NO20022171L (no) | 2002-07-04 |
NO331732B1 NO331732B1 (no) | 2012-03-12 |
Family
ID=14237231
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20022171A NO331732B1 (no) | 1999-11-08 | 2002-05-07 | Fremgangsmate for syntese av nukleinsyre |
Country Status (9)
Country | Link |
---|---|
KR (1) | KR100612551B1 (no) |
CN (2) | CN100393875C (no) |
BR (1) | BR0015382B1 (no) |
CA (1) | CA2390309C (no) |
IL (1) | IL149446A0 (no) |
NO (1) | NO331732B1 (no) |
RU (1) | RU2252964C2 (no) |
WO (1) | WO2001034790A1 (no) |
ZA (1) | ZA200203293B (no) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US8445664B2 (en) | 1998-06-24 | 2013-05-21 | Enzo Diagnostics, Inc. | Kits for amplifying and detecting nucleic acid sequences |
ES2464132T3 (es) * | 2000-04-07 | 2014-05-30 | Eiken Kagaku Kabushiki Kaisha | Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario |
ATE437225T1 (de) | 2000-09-19 | 2009-08-15 | Eiken Chemical | Verfahren zur polynukleotidsynthese |
KR100806680B1 (ko) * | 2003-11-21 | 2008-02-26 | 한국표준과학연구원 | Pcr용 열순환 반응기의 성능시험용 다중 pcr 조성물 |
PT2412718T (pt) * | 2009-03-26 | 2016-12-30 | Xiamen Amoy Diagnostics Co Ltd | Iniciador em forma de anel utilizado na amplificação de ácido nucleico e a sua utilização |
CN101671674B (zh) * | 2009-03-27 | 2010-09-22 | 郑立谋 | 一种用于核酸扩增的环形引物及其应用 |
TWI600766B (zh) | 2012-08-09 | 2017-10-01 | 財團法人工業技術研究院 | 用於偵測一目標核苷酸序列中之一特定區域的一突變及/或多形性的套組 |
EP2970951B1 (en) | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
SG11201507261WA (en) | 2013-03-15 | 2015-10-29 | Theranos Inc | Nucleic acid amplification |
SG11201507272RA (en) | 2013-03-15 | 2015-10-29 | Theranos Inc | Nuclei acid amplification |
ES2703792T3 (es) * | 2013-11-22 | 2019-03-12 | Orion Diagnostica Oy | Detección de ácidos nucleicos mediante amplificación basada en invasión de hebra |
EP3169793B1 (en) | 2014-07-16 | 2022-07-06 | Tangen Biosciences Inc. | Isothermal methods for amplifying nucleic acid samples |
US11873480B2 (en) * | 2014-10-17 | 2024-01-16 | Illumina Cambridge Limited | Contiguity preserving transposition |
US11559801B2 (en) | 2014-11-03 | 2023-01-24 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
ES2764975T3 (es) * | 2014-12-15 | 2020-06-05 | Cepheid | Amplificación de ácidos nucleicos en base exponencial mayor de 2 |
WO2018132939A1 (zh) * | 2017-01-17 | 2018-07-26 | 中国科学院过程工程研究所 | 一种恒温条件下合成核酸的方法 |
EP3476938B1 (en) * | 2017-09-14 | 2020-05-13 | Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. | Method and kit for synthesizing nucleic acid under constant temperature conditions |
EP3874064A1 (en) | 2018-10-29 | 2021-09-08 | Cepheid | Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers |
US20230183819A1 (en) | 2019-01-15 | 2023-06-15 | Neogen Food Safety Us Holdco Corporation | Loop-mediated isothermal amplification primers for shiga toxin-producing e. coli (stec) detection |
US20230119314A1 (en) | 2020-02-17 | 2023-04-20 | Neogen Food Safety Us Holdco Corporation | Loop-mediated isothermal amplification primers for vibrio parahaemolyticus detection and uses thereof |
TW202219272A (zh) | 2020-06-12 | 2022-05-16 | 美商夏洛克生物科學公司 | SARS-CoV-2之偵測 |
EP4193363A1 (en) | 2020-08-07 | 2023-06-14 | Oxford Nanopore Technologies plc | Methods of identifying nucleic acid barcodes |
CN113201583B (zh) * | 2021-04-29 | 2022-02-08 | 国科宁波生命与健康产业研究院 | 恒温条件下合成核酸的方法及试剂盒和应用 |
EP4105327A4 (en) * | 2021-04-29 | 2023-01-18 | Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences | PROCESS FOR THE PREPARATION OF CONSTANT TEMPERATURE NUCLEIC ACIDS AND KIT AND USE THEREOF |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4213029A1 (de) * | 1991-09-26 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen |
CA2080305A1 (en) * | 1991-10-11 | 1993-04-12 | Linda M. Western | Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification |
WO1993017127A1 (en) * | 1992-02-20 | 1993-09-02 | The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Boomerand dna amplification |
FR2721945B1 (fr) * | 1994-07-04 | 1996-10-18 | David Fabrice | Accroissement genique, un procede d'amplicication genique isotherme et ses applications |
FR2726277B1 (fr) * | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
ATE317023T1 (de) * | 1999-11-08 | 2006-02-15 | Eiken Chemical | Methode zum nachweis von variationen oder polymorphismen |
-
2000
- 2000-03-28 KR KR1020027005901A patent/KR100612551B1/ko active IP Right Grant
- 2000-03-28 CN CNB008182620A patent/CN100393875C/zh not_active Expired - Lifetime
- 2000-03-28 IL IL14944600A patent/IL149446A0/xx unknown
- 2000-03-28 CA CA2390309A patent/CA2390309C/en not_active Expired - Lifetime
- 2000-03-28 RU RU2002115268/13A patent/RU2252964C2/ru active
- 2000-03-28 WO PCT/JP2000/001919 patent/WO2001034790A1/ja active IP Right Grant
- 2000-03-28 BR BRPI0015382-6A patent/BR0015382B1/pt active IP Right Grant
- 2000-11-07 CN CN2006100803797A patent/CN1876843B/zh not_active Expired - Lifetime
-
2002
- 2002-04-25 ZA ZA200203293A patent/ZA200203293B/xx unknown
- 2002-05-07 NO NO20022171A patent/NO331732B1/no not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
CN1876843A (zh) | 2006-12-13 |
RU2252964C2 (ru) | 2005-05-27 |
CA2390309A1 (en) | 2001-05-17 |
ZA200203293B (en) | 2003-03-26 |
BR0015382B1 (pt) | 2014-04-29 |
RU2002115268A (ru) | 2004-01-27 |
NO20022171D0 (no) | 2002-05-07 |
KR100612551B1 (ko) | 2006-08-11 |
WO2001034790A1 (fr) | 2001-05-17 |
CN100393875C (zh) | 2008-06-11 |
NO331732B1 (no) | 2012-03-12 |
CN1876843B (zh) | 2012-09-05 |
BR0015382A (pt) | 2002-07-02 |
IL149446A0 (en) | 2002-11-10 |
CA2390309C (en) | 2012-09-25 |
CN1420928A (zh) | 2003-05-28 |
KR20020064896A (ko) | 2002-08-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
NO20022171L (no) | Fremgangsmåte for syntese av nukleinsyre | |
Levin et al. | An alternative procedure for the aluminum-mediated conversion of esters to amides | |
MY157626A (en) | Method of synthesizing nucleic acid | |
CY1109828T1 (el) | Κατασκευασμενα προτυπα και η χρηση τους στην ενισχυση μοναδικου εκκινητη | |
FI972992A (fi) | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten | |
FI911152A0 (fi) | Amplifikationsfoerfaranden av nukleinsyrasekvens. | |
HUP9800849A2 (hu) | Javított eljárás ciklopropil-acetilén előállítására | |
EP1275715A4 (en) | METHOD OF AMPLIFYING NUCLEIC ACID USING DOUBLE-STRANDED NUCLEIC ACID AS A MODEL | |
NZ515673A (en) | Compositions and methods for labeling of nucleic acid molecules | |
WO2002046450A3 (en) | Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction | |
EA200200092A1 (ru) | Способ получения алканов с использованием метана | |
HUP0300856A2 (hu) | Eljárás szubsztituált fenil-acetonitrilek elżállítására | |
BR0213814A (pt) | Processos para a fabricação de 3-hidroxi-n, 1,6-trialquil-4-oxo-1, 4-diidro piridino-2-carboxamida | |
BR0213779A (pt) | Molécula de ácido nucleico isolada, vetor, célula hospedeira, e, método para a preparação de um composto de quìmica fina | |
BG103857A (en) | Method for the preparation of sertindol | |
PT1343735E (pt) | Processo para a preparacao de carboneto de volframio | |
HUP0004510A1 (hu) | Eljárás L-dihidroorotsav előállítására és a vegyület alkalmazása | |
ATE347609T1 (de) | Enzymatisches verfahren zur herstellung von organofluorverbindungen | |
MXPA03010722A (es) | Reacciones de adicion no catalizadas. | |
AU2001264493A1 (en) | Solid phase synthesis of cyanine dyes | |
BR0312773A (pt) | Método para produzir um composto altamente puro | |
IL133719A0 (en) | Method for the production of n-(5-amino-2-cyano-4- fluorophenyl) - sulphonamides and new intermediate products | |
DE60114775D1 (de) | Verfahren zur herstellung von bisindenylalkanen | |
Kacian et al. | Kits for nucleic acid sequence amplification methods | |
DK1280894T3 (da) | Fremgangsmåder til at danne rekombinerede nucleinsyrer |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK1K | Patent expired |